Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 766 55.6 2631458 94.7% 2491990 109.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,255,654 C→T L271L (CTG→CTA)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,255,6540CT100.0% 180.9 / NA 56L271L (CTG→CTA) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/28);  total (28/28)

GAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCT  >  NC_000913/4255520‑4255783
                                                                                                                                      |                                                                                                                                 
gAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGaa                                                                                                                               <  2:660160/139‑1 (MQ=255)
        cagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGG‑CAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcag                                                                                                                      <  2:763618/139‑1 (MQ=255)
          ggTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagt                                                                                                                     <  1:300848/139‑1 (MQ=255)
               tCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGc                                                                                                                <  2:925966/139‑1 (MQ=255)
               tCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGc                                                                                                                <  2:724564/139‑1 (MQ=255)
                 cATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGa                                                                                                              <  2:1260848/139‑1 (MQ=255)
                   tgtgACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAat                                                                                                            <  2:1240833/139‑1 (MQ=255)
                      gACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAatcat                                                                                                         >  1:608874/1‑139 (MQ=255)
                             gTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTaa                                                                                                  >  1:525757/1‑139 (MQ=255)
                                             aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  2:742847/139‑1 (MQ=255)
                                             aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  2:50005/139‑1 (MQ=255)
                                             aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  2:431636/139‑1 (MQ=255)
                                             aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  2:383855/139‑1 (MQ=255)
                                               cAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAtc                                                                                >  2:258857/1‑139 (MQ=255)
                                                aGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagtc                                                                                                                    >  1:499726/1‑102 (MQ=255)
                                                aGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagtc                                                                                                                    <  2:499726/102‑1 (MQ=255)
                                                  cTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                                                             <  1:258857/139‑1 (MQ=255)
                                                  cTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                                                             >  2:929361/1‑139 (MQ=255)
                                                  cTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                                                             >  2:985995/1‑139 (MQ=255)
                                                   tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAatca                                                                                                          >  2:830331/1‑109 (MQ=255)
                                                   tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAatca                                                                                                          <  1:830331/109‑1 (MQ=255)
                                                     aTGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTcc                                                                          <  2:608874/139‑1 (MQ=255)
                                                     aTGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTcc                                                                          >  1:1003315/1‑139 (MQ=255)
                                                      tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagtca                                                                                                                   >  1:1125532/1‑97 (MQ=255)
                                                      tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgtcagtca                                                                                                                   <  2:1125532/97‑1 (MQ=255)
                                                      tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgt                                                                                                                         >  1:1255845/1‑91 (MQ=255)
                                                      tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGgt                                                                                                                         <  2:1255845/91‑1 (MQ=255)
                                                       gACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt                                                                        >  1:819933/1‑139 (MQ=255)
                                                                    cAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg                                                           <  2:908675/139‑1 (MQ=255)
                                                                       cTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc                                                        >  1:814149/1‑139 (MQ=255)
                                                                            gAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctgctg                                                   >  2:628208/1‑139 (MQ=255)
                                                                                    tCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt                                           >  1:1276326/1‑139 (MQ=255)
                                                                                     cAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  >  2:1270361/1‑99 (MQ=255)
                                                                                     cAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                                                  <  1:1270361/99‑1 (MQ=255)
                                                                                                    tGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                                                     <  1:357721/97‑1 (MQ=255)
                                                                                                    tGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                                                     >  2:357721/1‑97 (MQ=255)
                                                                                                    tGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg                                                           >  2:62465/1‑107 (MQ=255)
                                                                                                    tGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg                                                           <  1:62465/107‑1 (MQ=255)
                                                                                                     gATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTtg                          >  2:742419/1‑139 (MQ=255)
                                                                                                           cTGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgc                    >  2:962581/1‑139 (MQ=255)
                                                                                                            tGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcg                   >  2:495083/1‑139 (MQ=255)
                                                                                                            tGGTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTgcgcg                   <  2:1276326/139‑1 (MQ=255)
                                                                                                              gTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTg                                                                   <  1:826335/89‑1 (MQ=255)
                                                                                                              gTAAAGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTg                                                                   >  2:826335/1‑89 (MQ=255)
                                                                                                                 aaGTCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCa              <  1:929361/139‑1 (MQ=255)
                                                                                                                    tCGGTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCAtct           >  1:412439/1‑139 (MQ=255)
                                                                                                                      ggTTCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCAtcttc         >  2:165839/1‑139 (MQ=255)
                                                                                                                        ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAagag                                                                                               >  2:953678/1‑51 (MQ=255)
                                                                                                                        ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAagag                                                                                               <  1:953678/51‑1 (MQ=255)
                                                                                                                        ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCATTCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTa       >  1:268374/1‑139 (MQ=255)
                                                                                                                        ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctg                                                      <  2:628934/92‑1 (MQ=255)
                                                                                                                        ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctg                                                      >  1:628934/1‑92 (MQ=255)
                                                                                                                        ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTtgct                        <  1:519107/122‑1 (MQ=255)
                                                                                                                        ttCCAGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTtgct                        >  2:519107/1‑122 (MQ=255)
                                                                                                                            aGGTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGc   <  2:814149/139‑1 (MQ=255)
                                                                                                                             ggTGAAGCCTAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCt  >  2:643088/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                 
GAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCT  >  NC_000913/4255520‑4255783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A