Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 766 55.6 2631458 94.7% 2491990 109.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,0861.G96.6% 200.0 / ‑0.3 59noncoding (80/87 nt)leuVtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (27/30);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

TTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGG  >  NC_000913/4605957‑4606215
                                                                                                                                  |                                                                                                                                 
ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑ggggtgggg                                                                                                                          >  1:132301/1‑138 (MQ=255)
ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑ggggggggg                                                                                                                          >  1:1081163/1‑138 (MQ=255)
ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑ggggggggg                                                                                                                          >  1:557524/1‑138 (MQ=255)
             ttttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              <  2:359353/139‑1 (MQ=255)
                        tCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                   <  2:132301/139‑1 (MQ=255)
                        acGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                   >  2:660219/2‑139 (MQ=255)
                            gCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGt                                                                                                       <  1:659548/131‑1 (MQ=255)
                            gCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGt                                                                                                       >  2:659548/1‑131 (MQ=255)
                             ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                              <  1:683722/139‑1 (MQ=255)
                              cTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                             <  2:557524/139‑1 (MQ=255)
                               tGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            <  2:914865/139‑1 (MQ=255)
                               tGGCACTTATTGCTGACATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGa                                                                                                                     <  1:1166515/114‑1 (MQ=255)
                               tGGCACTTATTGCTGACATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGa                                                                                                                     >  2:1166515/1‑114 (MQ=255)
                                 gCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca                                                                                          >  1:346206/1‑139 (MQ=255)
                                  cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                                         >  2:419131/1‑139 (MQ=255)
                                     ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg                                                                                      <  1:1156119/139‑1 (MQ=255)
                                               catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                            >  1:488076/1‑139 (MQ=255)
                                                   atGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                        >  1:660407/1‑139 (MQ=255)
                                                        gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              <  2:845759/96‑1 (MQ=255)
                                                        gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              >  1:845759/1‑96 (MQ=255)
                                                              aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                             <  2:200306/139‑1 (MQ=255)
                                                              aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                             >  2:612227/1‑139 (MQ=255)
                                                               aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              >  2:999555/1‑89 (MQ=255)
                                                               aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              <  1:999555/89‑1 (MQ=255)
                                                               aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            >  2:430887/1‑107 (MQ=255)
                                                               aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            <  1:430887/107‑1 (MQ=255)
                                                                tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                           <  1:721511/139‑1 (MQ=255)
                                                                        aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGa                                                                                                                     <  2:501947/73‑1 (MQ=255)
                                                                        aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGa                                                                                                                     >  1:501947/1‑73 (MQ=255)
                                                                        aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                   >  2:125256/1‑139 (MQ=255)
                                                                        aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca                                                   >  1:852494/1‑139 (MQ=255)
                                                                         aTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                  <  1:660219/139‑1 (MQ=255)
                                                                         aTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                  <  2:660407/139‑1 (MQ=255)
                                                                           cAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAg                                                <  2:346206/139‑1 (MQ=255)
                                                                            aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑ggggg                                                                                                                              <  1:386560/59‑1 (MQ=255)
                                                                            aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑ggggg                                                                                                                              >  2:386560/1‑59 (MQ=255)
                                                                            aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTc                                              >  2:508260/1‑139 (MQ=255)
                                                                                 tGAAAAATCAAGGAAGAAAAAAGAAAGGAAGTAAAGATAATTGCGGCGAGGGGGGGGGACTTGAACCCCCACGTCCCTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCt                                          <  2:852494/139‑1 (MQ=21)
                                                                                  gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCtt                                         <  2:488076/139‑1 (MQ=255)
                                                                                        tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACtttt                                   >  1:1304877/1‑139 (MQ=255)
                                                                                         cAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                    <  1:176466/73‑1 (MQ=255)
                                                                                         cAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                    >  2:176466/1‑73 (MQ=255)
                                                                                            ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              >  1:350105/1‑60 (MQ=37)
                                                                                            ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              <  2:350105/60‑1 (MQ=37)
                                                                                            ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTg                               >  2:1204247/1‑139 (MQ=255)
                                                                                              aagaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGat                             <  2:965252/139‑1 (MQ=255)
                                                                                               agaaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGata                            <  1:125256/139‑1 (MQ=255)
                                                                                                 aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                          >  1:1088141/1‑139 (MQ=255)
                                                                                                   aCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                        <  2:368935/139‑1 (MQ=255)
                                                                                                   aCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                        >  1:368935/1‑139 (MQ=255)
                                                                                                   aCAAGAAAGGAAGTAAAGATAATCGGTGCGAGGGGGGGGGACTTGAACCCCCACCTCCGTAAGGACACTAACACCTGAACCTAGAGCGTCTACCAATTCAGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                        <  2:866634/139‑1 (MQ=21)
                                                                                                        aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCGATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGt                   >  2:75392/1‑139 (MQ=255)
                                                                                                           ggAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              <  1:954643/45‑1 (MQ=21)
                                                                                                           ggAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCa                                                                                                              >  2:954643/1‑45 (MQ=21)
                                                                                                           ggAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                             <  2:250374/94‑1 (MQ=255)
                                                                                                           ggAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                             >  1:250374/1‑94 (MQ=255)
                                                                                                             aaGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg             <  1:508260/139‑1 (MQ=255)
                                                                                                              aGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg             >  1:718300/1‑139 (MQ=255)
                                                                                                                 aaaGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa          <  2:1081163/139‑1 (MQ=255)
                                                                                                                         ttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca                                                 <  2:903761/92‑1 (MQ=12)
                                                                                                                         ttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca                                                 >  1:903761/1‑92 (MQ=12)
                                                                                                                         ttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggg  >  1:988009/1‑139 (MQ=255)
                                                                                                                             tGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                        <  2:53377/113‑1 (MQ=255)
                                                                                                                             tGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                        >  1:53377/1‑113 (MQ=255)
                                                                                                                                  |                                                                                                                                 
TTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGG  >  NC_000913/4605957‑4606215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A