Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
778 |
68.7 |
3228504 |
93.4% |
3015422 |
109.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,102,221 |
T→C |
E107E (GAA→GAG) |
csgE ← |
curlin secretion specificity factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,102,221 | 0 | T | C | 100.0%
| 227.9
/ NA
| 64 | E107E (GAA→GAG) | csgE | curlin secretion specificity factor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/31); total (33/31) |
TGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGG > NC_000913/1102097‑1102348
|
tGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAAt < 2:885999/139‑1 (MQ=255)
aGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCa > 2:1310887/1‑139 (MQ=255)
aaCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCa > 1:703885/1‑139 (MQ=255)
actactGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCaa > 1:1565508/1‑139 (MQ=255)
actactGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCaa > 1:460212/1‑139 (MQ=255)
ctGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCa < 2:152808/119‑1 (MQ=255)
ctGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCa > 1:152808/1‑119 (MQ=255)
ctGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAgac > 1:526224/1‑139 (MQ=255)
ttGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGttt > 2:1072821/1‑139 (MQ=255)
aTTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCg > 2:352632/1‑135 (MQ=255)
aTTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCg < 1:352632/135‑1 (MQ=255)
aTTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt > 1:326356/1‑139 (MQ=255)
cAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTctct > 1:970135/1‑139 (MQ=255)
tCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTg > 2:14295/1‑98 (MQ=255)
tCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTg < 1:14295/98‑1 (MQ=255)
cGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc < 1:1310887/139‑1 (MQ=255)
tttatttaGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATaaaa < 1:290053/139‑1 (MQ=255)
aGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTg > 2:1187047/1‑139 (MQ=255)
aaTTCATCATGCGCCAAATCGCCTGTACTTAATAACGCCTGAATTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTgca < 1:1044877/139‑3 (MQ=255)
catcatGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAAt < 2:526224/139‑1 (MQ=255)
atcatGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAgac > 1:1084089/1‑87 (MQ=255)
atcatGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAgac < 2:1084089/87‑1 (MQ=255)
atcatGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATa > 1:1406490/1‑139 (MQ=255)
tcatGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGaa < 2:1302196/118‑1 (MQ=255)
tcatGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGaa > 1:1302196/1‑118 (MQ=255)
gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCa < 1:187986/62‑1 (MQ=255)
gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCa > 2:187986/1‑62 (MQ=255)
gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTc > 2:1537152/1‑139 (MQ=255)
aTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTg < 2:750754/139‑1 (MQ=255)
tCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa < 2:746768/139‑1 (MQ=255)
tCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa < 2:326356/139‑1 (MQ=255)
cGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc < 1:346783/139‑1 (MQ=255)
aCTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt < 1:1314133/129‑1 (MQ=255)
aCTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt > 2:1314133/1‑129 (MQ=255)
aCTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTtata < 1:1072821/139‑1 (MQ=255)
cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc > 1:789136/1‑85 (MQ=255)
cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc < 2:789136/85‑1 (MQ=255)
cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt < 1:813759/120‑1 (MQ=255)
cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt > 2:813759/1‑120 (MQ=255)
cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAg < 2:305557/139‑1 (MQ=255)
cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAg > 2:527324/1‑139 (MQ=255)
ccTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAAt > 2:1126706/1‑43 (MQ=255)
ccTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAAt < 1:1126706/43‑1 (MQ=255)
ccTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCt > 2:799966/1‑139 (MQ=255)
cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt > 2:1051481/1‑71 (MQ=255)
cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTcc < 2:644136/112‑1 (MQ=255)
cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTcc > 1:644136/1‑112 (MQ=255)
cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCtt > 2:1241321/1‑139 (MQ=255)
cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCtt > 1:926951/1‑139 (MQ=255)
cTGATTTATCTGGCGACGATTTAGTGACTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt < 1:1051481/71‑1 (MQ=255)
gATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc < 2:1565508/139‑1 (MQ=255)
gATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc < 2:970135/139‑1 (MQ=255)
tCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt < 2:196389/63‑1 (MQ=255)
tCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt > 1:196389/1‑63 (MQ=255)
ggCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAGAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCACCATCGTGc > 1:950658/1‑139 (MQ=255)
cgacgaTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAgac < 2:418631/41‑1 (MQ=255)
cgacgaTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAgac > 1:418631/1‑41 (MQ=255)
cgacgaTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc < 2:13001/107‑1 (MQ=255)
cgacgaTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc > 1:13001/1‑107 (MQ=255)
cgaTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTgg < 1:527324/139‑1 (MQ=255)
aTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGa < 2:1554026/50‑1 (MQ=255)
aTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGa > 1:1554026/1‑50 (MQ=255)
aTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCAc > 2:1497731/1‑134 (MQ=255)
aTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCAc < 1:1497731/134‑1 (MQ=255)
|
TGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGG > NC_000913/1102097‑1102348
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
