Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,672,639	T→C	L227P (CTG→CCG)	ydgD →	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,672,639	0	T	C	100.0%	191.2 / NA	53	L227P (CTG→CCG)	ydgD	putative peptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/26); total (27/26)

AGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGT  >  NC_000913/1672510‑1672770

aggcaggcTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCa                                                                                                                            <  1:293661/139‑1 (MQ=255)

                aGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTgg                                                                                                            >  2:122491/1‑139 (MQ=255)

                aGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTgg                                                                                                            >  2:1155536/1‑139 (MQ=255)

                aGATCATCTCGATACGTTGTACAGTCATCAAAACTGAGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTgg                                                                                                            <  2:1012355/139‑1 (MQ=255)

                    catcTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGc                                                                                                               >  2:671533/1‑132 (MQ=255)

                    catcTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGc                                                                                                               <  1:671533/132‑1 (MQ=255)

                    catcTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCaat                                                                                                        >  1:1447140/1‑139 (MQ=255)

                       ctcGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGAt                                                                                                                                  >  1:1311459/1‑110 (MQ=255)

                       ctcGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGAt                                                                                                                                  <  2:1311459/110‑1 (MQ=255)

                       ctcGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCaattaa                                                                                                     <  1:1479821/139‑1 (MQ=255)

                         cGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCaattaatt                                                                                                   <  2:1117593/139‑1 (MQ=255)

                          gATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTg                                                                                                  <  2:1035319/139‑1 (MQ=255)

                                 tGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATa                                                                                                                          >  1:1481764/1‑108 (MQ=255)

                                 tGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATa                                                                                                                          <  2:1481764/108‑1 (MQ=255)

                                 tGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTggg                                                                                                <  2:307382/134‑1 (MQ=255)

                                 tGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTggg                                                                                                >  1:307382/1‑134 (MQ=255)

                                          atcaAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCa                                                                                                          >  2:660244/1‑115 (MQ=255)

                                          atcaAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCa                                                                                                          <  1:660244/115‑1 (MQ=255)

                                          atcaAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGc                                                                                  >  1:723509/1‑139 (MQ=255)

                                          atcaAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGc                                                                                  >  2:296830/1‑139 (MQ=255)

                                          atcaAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGc                                                                                  >  1:1110856/1‑139 (MQ=255)

                                          atcaAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTGGCCGGGTGACAGCGGTTCGCCTCCGATGTTGGATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTAGGc                                                                                  >  1:1047762/1‑139 (MQ=255)

                                               aaCTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTg                                                                             <  2:1110856/139‑1 (MQ=255)

                                                  tgtgGAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCcg                                                                          >  1:1065351/1‑139 (MQ=255)

                                                         tGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTg                                                                                                  >  2:284188/1‑108 (MQ=255)

                                                         tGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTg                                                                                                  <  1:284188/108‑1 (MQ=255)

                                                           aCTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATc                                                                 >  1:726701/1‑139 (MQ=255)

                                                           aCTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATc                                                                 >  1:517050/1‑139 (MQ=255)

                                                                ctggGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATg                                                                                                                    <  2:435533/83‑1 (MQ=255)

                                                                ctggGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATg                                                                                                                    >  1:435533/1‑83 (MQ=255)

                                                                 tggGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGgc                                                           <  2:954613/139‑1 (MQ=255)

                                                                 tggGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGgc                                                           <  2:1447140/139‑1 (MQ=255)

                                                                 tggGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGgc                                                           <  1:604665/139‑1 (MQ=255)

                                                                  gggCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTACGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGgcg                                                          <  1:1155536/139‑1 (MQ=255)

                                                                   ggCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCcgc                                                                         >  2:482329/1‑123 (MQ=255)

                                                                   ggCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCcgc                                                                         <  1:482329/123‑1 (MQ=255)

                                                                     cgcAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGgcgcgc                                                       >  2:494744/1‑139 (MQ=255)

                                                                                               tGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCg                             >  1:752282/1‑139 (MQ=255)

                                                                                                gCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCt                                                                                                              >  2:1591213/1‑57 (MQ=255)

                                                                                                gCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCt                                                                                                              <  1:1591213/57‑1 (MQ=255)

                                                                                                gCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCgg                            >  1:563281/1‑139 (MQ=255)

                                                                                                 cGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCg                                              >  2:260814/1‑120 (MQ=255)

                                                                                                 cGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCg                                              <  1:260814/120‑1 (MQ=255)

                                                                                                 cGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTAACGGt                           >  1:598669/1‑139 (MQ=255)

                                                                                                    tACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGtttt                        <  1:296830/139‑1 (MQ=255)

                                                                                                         tGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGgcg                                                          >  1:860860/1‑100 (MQ=255)

                                                                                                         tGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGgcg                                                          <  2:860860/100‑1 (MQ=255)

                                                                                                            cGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACaa                <  1:682514/139‑1 (MQ=255)

                                                                                                                tGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCaa                                                                                          >  1:473655/1‑61 (MQ=255)

                                                                                                                tGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCaa                                                                                          <  2:473655/61‑1 (MQ=255)

                                                                                                                  aCAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGa          <  1:1020793/139‑1 (MQ=255)

                                                                                                                    aGCGGTTCGCCTCCGATTTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATc        <  2:1065351/139‑1 (MQ=255)

                                                                                                                     gCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCa       >  1:1165673/1‑139 (MQ=255)

                                                                                                                          tCGCCTACGATCTTGAATACCGATGACGGCTGGCAATTAATTGTGGTGCAAAGTTCGGCTCCTGCCGCGACAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGTTCAACTGt  <  2:1047762/139‑1 (MQ=255)

AGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGT  >  NC_000913/1672510‑1672770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A