Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,672,639	T→C	L227P (CTG→CCG)	ydgD →	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,672,639	0	T	C	100.0%	79.1 / NA	23	L227P (CTG→CCG)	ydgD	putative peptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/8); total (15/8)

GGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAA  >  NC_000913/1672511‑1672756

ggcaggcTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAACACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCAt                                                                                                             >  1:653651/1‑139 (MQ=255)

           cTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACgg                                                                                                  <  2:468100/139‑1 (MQ=255)

           cTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACgg                                                                                                  <  1:143037/139‑1 (MQ=255)

               aGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTgg                                                                                              <  1:30452/139‑1 (MQ=255)

               aGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTgg                                                                                              >  1:432040/1‑139 (MQ=255)

               aGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTgg                                                                                              >  2:30452/1‑139 (MQ=255)

                                         atcaAAACTGTGAAGTGACTTGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCgtc                                                                    >  2:453217/1‑137 (MQ=255)

                                            aaaaCTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTcc                                                                 >  1:300660/1‑139 (MQ=255)

                                                    gAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGa                                                         >  2:763623/1‑139 (MQ=255)

                                                                 gggCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGgcg                                            <  2:432040/139‑1 (MQ=255)

                                                                    cgcAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTg                                                               <  1:189951/117‑1 (MQ=255)

                                                                    cgcAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTg                                                               >  2:189951/1‑117 (MQ=255)

                                                                                     tCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCAttt                        >  1:9647/1‑139 (MQ=255)

                                                                                          tCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGtt                   >  2:731340/1‑139 (MQ=255)

                                                                                             gTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTAcc                >  1:634328/1‑139 (MQ=255)

                                                                                                  aTACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAAc                                  >  1:715902/1‑116 (MQ=255)

                                                                                                  aTACCTTGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAAc                                  <  2:715902/116‑1 (MQ=255)

                                                                                                        tggcGGGTGACCGCGGTTCGCCTCCGATGTTGCATACCGATGACGCCTGGCATTTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGa     >  2:446705/4‑139 (MQ=255)

                                                                                                        tGCCGGGTGACAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGa     >  2:571457/1‑139 (MQ=255)

                                                                                                                  cAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACaa  <  2:170465/132‑1 (MQ=255)

                                                                                                                  cAGCGGTTCGCCTCCGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACaa  >  1:170465/1‑132 (MQ=255)

                                                                                                                          cGCCTCCGATGTTGGATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCggg                              >  1:467929/1‑96 (MQ=255)

                                                                                                                          cGCCTCCGATGTTGCAGACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCggg                              <  2:467929/96‑1 (MQ=255)

GGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAA  >  NC_000913/1672511‑1672756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A