Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,045	T→C	K164E (AAA→GAA)	proQ ←	RNA chaperone, putative ProP translation regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,045	0	T	C	100.0%	125.5 / NA	37	K164E (AAA→GAA)	proQ	RNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/15); total (22/15)

TCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG  >  NC_000913/1914915‑1915171

tCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGTTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCg                                                                                                                        >  1:1495806/1‑139 (MQ=255)

         gTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTgcg                                                                                                               >  1:1033598/1‑139 (MQ=255)

         gTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTgca                                                                                                               >  1:803450/1‑138 (MQ=255)

          tGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTgcgc                                                                                                              >  2:961805/1‑139 (MQ=255)

          tGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTgcgc                                                                                                              >  1:699568/1‑139 (MQ=255)

           ggCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCa                                                                                                             >  2:160630/1‑139 (MQ=255)

            gCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCAc                                                                                                            <  2:699568/139‑1 (MQ=255)

            gCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCAc                                                                                                            <  2:236133/139‑1 (MQ=255)

            acagccatcGCGTTTTGACCCGCTTTCACCTTCTGTGCTTGTCCGACAGCCAGAGCTGAAATGTCAGAAACCGGGGTGTGATGGTCTTCGCGAGGTGCTTTTACGGTTTTTGGCGATTCATCTACCGGCTTTTGCGCAc                                                                                                            <  1:296979/135‑1 (MQ=255)

               tccatcGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAg                                                                                                         >  1:30177/1‑139 (MQ=255)

               tccatcGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAg                                                                                                         >  2:822944/1‑139 (MQ=255)

                    cgcgTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTa                                                                                                    >  2:942993/1‑139 (MQ=255)

                           tGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGtctgt                                                                                             >  2:296979/1‑135 (MQ=255)

                              cccGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAgcgc                                                                                          <  1:822944/139‑1 (MQ=255)

                                       aCCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTgc                                                                                 >  1:185384/1‑139 (MQ=255)

                                             aGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCggcgtg                                                                           <  1:160630/139‑1 (MQ=255)

                                               ggCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTg                                                                                  >  1:1487966/1‑130 (MQ=255)

                                               ggCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTg                                                                                  <  2:1487966/130‑1 (MQ=255)

                                                   tGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAg                                                                     >  1:1493999/1‑139 (MQ=255)

                                                   tGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAg                                                                     >  2:124563/1‑139 (MQ=255)

                                                    gTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGt                                                                    <  2:30177/139‑1 (MQ=255)

                                                    gTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGt                                                                    <  2:1377920/139‑1 (MQ=255)

                                                               agagCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCt                                                         >  2:374234/1‑139 (MQ=255)

                                                                   cTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCg                                                     >  1:1275180/1‑139 (MQ=255)

                                                                             aGAAACCGGGGTGTGCTGTTATTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgc                                           <  1:1499300/139‑1 (MQ=255)

                                                                                 aCCGGGGTGTGTTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTg                                       <  2:1495806/139‑1 (MQ=255)

                                                                                     gggTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTg                                                                                  >  1:877868/1‑92 (MQ=255)

                                                                                     gggTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTg                                                                                  <  2:877868/92‑1 (MQ=255)

                                                                                      ggTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcgg                                              >  2:347105/1‑127 (MQ=255)

                                                                                      ggTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcgg                                              <  1:347105/127‑1 (MQ=255)

                                                                                          tgCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTcttctt                              >  1:1553923/1‑139 (MQ=255)

                                                                                                    cgcgAGGTGCTTTTACTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACcagc                    >  1:1029529/1‑139 (MQ=255)

                                                                                                                  aCTGTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCtt      <  2:1029529/139‑1 (MQ=255)

                                                                                                                     gTTTTTGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGAGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTcgc   <  2:1553923/139‑1 (MQ=255)

                                                                                                                      tttttGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTtctc                          <  1:700414/115‑1 (MQ=255)

                                                                                                                      tttttGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTtctc                          >  2:700414/1‑115 (MQ=255)

                                                                                                                      tttttGGCGCTTCCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTcgcg  >  1:864701/1‑139 (MQ=255)

TCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG  >  NC_000913/1914915‑1915171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A