Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,916,944 |
A→G |
K229K (AAA→AAG) |
yebS → |
inner membrane PqiA domain protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,916,944 | 0 | A | G | 100.0%
| 170.3
/ NA
| 48 | K229K (AAA→AAG) | yebS | inner membrane PqiA domain protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/24); total (24/24) |
GTGTCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGG > NC_000913/1916820‑1917062
|
gtgtCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTgtctgtct > 1:446412/1‑139 (MQ=255)
tgtCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCtt > 1:64818/1‑139 (MQ=255)
tgtCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGAAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCtt > 1:827758/1‑139 (MQ=255)
ctctTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt < 2:827758/139‑1 (MQ=255)
ctctTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt < 1:137638/139‑1 (MQ=255)
ttttGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTAcc < 1:302217/139‑1 (MQ=255)
ttttGTGGCGTTGGTGATTCTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTAcc < 2:630710/139‑1 (MQ=255)
ttGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg < 1:960293/139‑1 (MQ=255)
ggTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGtgtct < 2:259404/107‑1 (MQ=255)
ggTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGtgtct > 1:259404/1‑107 (MQ=255)
ttttAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCg < 2:446412/139‑1 (MQ=255)
cgacgGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAAGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt > 1:221540/1‑113 (MQ=255)
cgacgGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAAGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt < 2:221540/113‑1 (MQ=255)
cgGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTg > 1:743969/1‑139 (MQ=255)
gTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGcc > 1:729003/1‑139 (MQ=255)
gACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg < 1:823460/139‑1 (MQ=255)
cGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcgcg < 2:1212328/139‑1 (MQ=255)
cGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcgcg < 1:137132/139‑1 (MQ=255)
tGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTg < 2:15283/139‑1 (MQ=255)
aTCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt < 1:566084/119‑1 (MQ=255)
aTCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGt > 2:566084/1‑119 (MQ=255)
cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCgtg > 2:137370/1‑65 (MQ=255)
cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCgtg < 1:137370/65‑1 (MQ=255)
cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTAt < 2:124798/94‑1 (MQ=255)
cGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTAt > 1:124798/1‑94 (MQ=255)
aagaaCTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCt > 1:946743/1‑139 (MQ=255)
aagaaCTGTGGGAGAGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCt > 1:751585/1‑139 (MQ=255)
cGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATa < 2:780138/139‑1 (MQ=255)
aTTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg < 1:257257/72‑1 (MQ=255)
aTTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg > 2:257257/1‑72 (MQ=255)
tttATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg > 2:1217791/1‑70 (MQ=255)
tttATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg < 1:1217791/70‑1 (MQ=255)
aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt > 2:973485/1‑56 (MQ=255)
aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCAtttt < 1:973485/56‑1 (MQ=255)
aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGt > 2:253143/1‑116 (MQ=255)
aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGt < 1:253143/116‑1 (MQ=255)
aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATg > 1:442645/1‑139 (MQ=255)
aGCGCCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCACTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATg > 2:826193/1‑139 (MQ=255)
cgcCCCGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCt > 2:946447/1‑139 (MQ=255)
ccccGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATa > 1:1316850/1‑122 (MQ=255)
ccccGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATa < 2:1316850/122‑1 (MQ=255)
cccGCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTggg > 2:679817/1‑139 (MQ=255)
gCTACGCGTAGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGggcgg < 2:743969/139‑1 (MQ=255)
aGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg > 2:580156/1‑72 (MQ=255)
aGGGACGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg < 1:580156/72‑1 (MQ=255)
cGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg < 2:624540/39‑1 (MQ=255)
cGAGAAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCgg > 1:624540/1‑39 (MQ=255)
gaAGCTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCcg > 1:1284003/1‑64 (MQ=255)
|
GTGTCGGCTTGTTCTCTTTTGTGGCGTTGGTGATTTTAACGACGGTGACGTTGTCACATCTTAATGTCGAAGAACTGTGGGAGCGATTTTATCCGCAGCGCCCCGCTACGCGTAGGGACGAGAAACTTCGTGTCTGTCTTGGGTGCCATTTTACCGGCTATCCAGATCAGCGTGGTCGCTGCCCGCGTTGCCATATCCCGCTACGCCTGCGTCGCCGTCATAGTCTGCAAAAATGCTGGGCGG > NC_000913/1916820‑1917062
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
