Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
778 |
68.7 |
3228504 |
93.4% |
3015422 |
109.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,232,752 |
G→A |
A161V (GCG→GTG) |
lysC ← |
lysine‑sensitive aspartokinase 3 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,232,752 | 0 | G | A | 98.0%
| 155.4
/ NA
| 49 | A161V (GCG→GTG) | lysC | lysine‑sensitive aspartokinase 3 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/26); minor base C (1/0); total (23/26) |
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGT > NC_000913/4232634‑4232867
|
gCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCa < 1:934647/139‑1 (MQ=255)
ggCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa < 1:1127697/139‑1 (MQ=255)
cAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCg < 2:114429/139‑1 (MQ=255)
ttGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGt < 1:570962/139‑1 (MQ=255)
tttATTTTAGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGATACGCATCACt > 2:1046836/1‑139 (MQ=255)
ttATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACtt > 1:1588267/1‑139 (MQ=255)
aTTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTa > 1:1148011/1‑139 (MQ=255)
tttCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg < 2:267075/139‑1 (MQ=255)
tttCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg < 2:1148011/139‑1 (MQ=255)
tttCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCAGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg < 2:564007/139‑1 (MQ=255)
cGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGACCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt < 1:249520/131‑1 (MQ=255)
cGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGACCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt > 2:249520/1‑131 (MQ=255)
aaaTCCCTGGGTGATCACTAAGCCTTCATGGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc < 2:1368398/139‑1 (MQ=255)
cTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGcc > 1:440997/1‑139 (MQ=255)
tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGt > 2:193333/1‑90 (MQ=255)
tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGt < 1:193333/90‑1 (MQ=255)
tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc < 1:45957/125‑1 (MQ=255)
tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc > 2:45957/1‑125 (MQ=255)
cACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACgg > 1:482796/1‑59 (MQ=255)
cACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACgg < 2:482796/59‑1 (MQ=255)
aaGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa > 2:1347123/1‑80 (MQ=255)
aaGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa < 1:1347123/80‑1 (MQ=255)
gCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt < 2:1054896/100‑1 (MQ=255)
gCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt > 1:1054896/1‑100 (MQ=255)
tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCCCGGCTCTATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCa > 1:1326814/1‑139 (MQ=255)
tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCg > 2:1554571/1‑77 (MQ=255)
tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCg < 1:1554571/77‑1 (MQ=255)
tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCa > 2:70224/1‑139 (MQ=255)
ttGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTTTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGa > 2:889965/1‑139 (MQ=255)
gagaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtc < 1:70224/139‑1 (MQ=255)
agaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtct < 1:336440/139‑1 (MQ=255)
tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTatat > 2:141797/1‑44 (MQ=255)
tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTatat < 1:141797/44‑1 (MQ=255)
tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTcaaca > 1:599448/1‑139 (MQ=255)
gggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCg < 2:1459547/65‑1 (MQ=255)
gggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCg > 1:1459547/1‑65 (MQ=255)
ggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAg > 1:494983/1‑127 (MQ=255)
ggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAg < 2:494983/127‑1 (MQ=255)
ggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGa > 1:350143/1‑129 (MQ=255)
ggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGa < 2:350143/129‑1 (MQ=255)
agcTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTa < 1:1465940/85‑1 (MQ=255)
agcTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTa > 2:1465940/1‑85 (MQ=255)
agcTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAAcagca < 2:440997/139‑1 (MQ=255)
tGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAggg > 1:1277521/1‑139 (MQ=255)
gCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGt < 2:599448/139‑1 (MQ=255)
gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCat < 1:1004909/65‑2 (MQ=255)
gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCAc > 2:1004909/1‑65 (MQ=255)
gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCgcgc < 2:675877/108‑1 (MQ=255)
gCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCgcgc > 1:675877/1‑108 (MQ=255)
|
GCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGT > NC_000913/4232634‑4232867
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
