Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I1 R1 778 68.7 3228504 93.4% 3015422 109.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,215 (G)8→7 noncoding (72/87 nt) leuP ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,2080G.100.0% 163.7 / NA 43noncoding (79/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/23);  total (20/23)

ATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606074‑4606341
                                                                                                                                       |                                                                                                                                     
aTAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                                    <  1:742181/139‑1 (MQ=255)
  aaTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggggg                                                                                                                                 >  2:1336978/1‑139 (MQ=255)
       gTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                                                           >  2:606980/1‑139 (MQ=255)
        tGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACCGTCATCTTACTTTTTTTGATATCTCTTCGTTTGGTGCGAgggggggccttga                                                                                                                           >  1:416321/1‑133 (MQ=18)
          cGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                                    <  2:142054/128‑1 (MQ=255)
          cGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                                    >  1:142054/1‑128 (MQ=255)
          cGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAAc                                                                                                                        >  1:577221/1‑139 (MQ=255)
                ggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCa                                                                                                                   <  1:606980/139‑1 (MQ=255)
                  ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg                                                                                                                 >  2:1208848/1‑139 (MQ=255)
                   gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCACCTTACTTTTTTTGATATCGCCTCGTTGGGTGCG‑GGGGGGGGACTTGAACCCTCACGt                                                                                                                >  1:898831/1‑139 (MQ=21)
                    ggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTc                                                                                                               <  2:663006/139‑1 (MQ=255)
                          gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                         >  1:577869/1‑139 (MQ=255)
                           aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                        <  1:436700/139‑2 (MQ=255)
                                ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                   <  1:1336978/139‑1 (MQ=255)
                                  aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                                 <  1:1208848/139‑1 (MQ=255)
                                        gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg                                                                                           >  2:1324184/1‑139 (MQ=255)
                                              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGa                                                                                                                          >  2:365870/1‑102 (MQ=255)
                                              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGa                                                                                                                          <  1:365870/102‑1 (MQ=255)
                                                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTCTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                                                                   <  1:1019124/139‑1 (MQ=255)
                                                   aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                                <  2:577869/139‑1 (MQ=255)
                                                       ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAAcc                                                                                                                       <  2:1503263/96‑1 (MQ=255)
                                                       ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAAcc                                                                                                                       >  1:1503263/1‑96 (MQ=255)
                                                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCCCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                   >  1:522583/1‑139 (MQ=255)
                                                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  2:522583/139‑1 (MQ=255)
                                                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg                                                                                                                 <  2:1170592/91‑1 (MQ=255)
                                                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg                                                                                                                 >  1:1170592/1‑91 (MQ=255)
                                                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  2:1530858/139‑1 (MQ=255)
                                                                            aaTTACGCAACCTAAACACAGTCATCTTACTTTTATTGATATCGGCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                       <  1:502512/139‑1 (MQ=17)
                                                                                  gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                 <  1:1324184/139‑1 (MQ=255)
                                                                                         tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggactt                                                                                                                             >  1:1444985/1‑53 (MQ=255)
                                                                                         tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggactt                                                                                                                             <  2:1444985/57‑5 (MQ=255)
                                                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg                                                                                                                 >  1:700229/1‑60 (MQ=255)
                                                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg                                                                                                                 <  2:700229/60‑1 (MQ=255)
                                                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                  >  1:365083/1‑139 (MQ=255)
                                                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  >  2:1230588/1‑106 (MQ=18)
                                                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  1:1230588/106‑1 (MQ=18)
                                                                                                  cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCCACGTgg                                 >  1:524065/1‑139 (MQ=255)
                                                                                                   aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt                                >  1:1613284/1‑139 (MQ=255)
                                                                                                         cTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg                                                                                                                 >  2:821770/1‑52 (MQ=255)
                                                                                                         cTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg                                                                                                                 <  1:821770/52‑1 (MQ=255)
                                                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                        >  1:626637/1‑139 (MQ=255)
                                                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                        >  1:157276/1‑139 (MQ=255)
                                                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCCCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                        <  2:898831/139‑1 (MQ=255)
                                                                                                            tttttGATACCGCCACGTTGGGGGCG‑GGGGGGGGACTTGAACCCCCACGCCCGTAAGGCGACTAACACCTGAAGCTAGCGCTTCTACCGATACCGCCACCCTCGCCCAGTGCGAGCAATATCAACGTGGTTATTGGTGc                       <  1:949374/139‑1 (MQ=18)
                                                                                                              tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   >  2:371737/1‑109 (MQ=255)
                                                                                                              tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   <  1:371737/109‑1 (MQ=255)
                                                                                                                  atatCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                 <  2:416321/139‑1 (MQ=255)
                                                                                                                   tatCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                <  2:577221/139‑1 (MQ=255)
                                                                                                                        ccTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCa                                                                                                                   >  2:1380351/1‑35 (MQ=17)
                                                                                                                        ccTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCa                                                                                                                   <  1:1380351/35‑1 (MQ=17)
                                                                                                                        ccTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc           >  2:809351/1‑139 (MQ=255)
                                                                                                                                 gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc  <  2:626637/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
ATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606074‑4606341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A