Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,606,215 |
(G)8→7 |
noncoding (72/87 nt) |
leuP ← |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,606,208 | 0 | G | . | 92.3%
| 165.6
/ 9.3
| 52 | noncoding (79/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base . (24/24); total (26/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCA > NC_000913/4606084‑4606343
|
cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAAc > 1:1518086/1‑139 (MQ=255)
ggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCa < 1:1356440/139‑1 (MQ=255)
cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg > 2:1274680/1‑117 (MQ=255)
cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg < 1:1274680/117‑1 (MQ=255)
aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg > 1:695939/1‑138 (MQ=255)
cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgga < 2:695939/139‑3 (MQ=255)
ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt > 2:1697956/1‑139 (MQ=255)
aCGTCCGTAAGGACACTAACACCTGAAGCTAGTGCGTCTACCAACTCCGCCACCTTCGTAAAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa < 1:1658943/139‑1 (MQ=37)
cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg < 2:1518086/139‑1 (MQ=255)
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc > 1:1656055/1‑139 (MQ=255)
cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc > 1:877728/1‑139 (MQ=255)
aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg > 1:982982/1‑139 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg > 1:1487400/1‑73 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg < 2:1487400/73‑1 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc > 2:1060090/1‑139 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 2:1690497/1‑138 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:1690497/138‑1 (MQ=255)
gcgTCTACCAATTCCGCCACCATCGTACAGTCATCTTACTGTTTTTGATATCGCCTCGTTGGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa < 2:466272/139‑1 (MQ=21)
tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGTTATCCCTTGGTTGGGTGCG‑GGGGGGGGACTTGAACCCCCACGTCGGTAAGGACCCTAACACCTGAACCTGGCGCGTCTACAAATCCCGCCCCCt > 2:1551390/1‑139 (MQ=18)
tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt > 1:1156381/1‑139 (MQ=255)
cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt > 1:1305556/1‑139 (MQ=255)
cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAg > 2:201048/1‑84 (MQ=255)
cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAg < 1:201048/84‑1 (MQ=255)
gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg < 1:1697956/139‑1 (MQ=255)
ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 2:75650/131‑1 (MQ=255)
ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc > 1:75650/1‑131 (MQ=255)
ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa > 2:252566/1‑139 (MQ=255)
aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGc < 2:982982/139‑1 (MQ=255)
ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 1:1276616/1‑116 (MQ=255)
ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:1276616/116‑1 (MQ=255)
ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat > 2:608409/1‑139 (MQ=255)
caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc < 1:608409/139‑1 (MQ=255)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggggg < 1:1606236/45‑1 (MQ=255)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggggg > 2:1606236/1‑45 (MQ=255)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg < 1:1025799/44‑1 (MQ=255)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg > 2:1025799/1‑44 (MQ=255)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAgga < 1:318289/70‑3 (MQ=25)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAgga > 2:318289/1‑68 (MQ=25)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAg < 2:161408/68‑1 (MQ=255)
tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAg > 1:161408/1‑68 (MQ=255)
cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt > 1:571591/1‑80 (MQ=21)
cATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt < 2:571591/80‑1 (MQ=18)
tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtt > 2:578559/1‑139 (MQ=255)
tACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt < 2:470057/139‑1 (MQ=255)
ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt < 2:1208791/71‑1 (MQ=17)
ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt > 1:1208791/1‑71 (MQ=17)
ttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc > 1:805655/1‑105 (MQ=255)
ttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 2:805655/105‑1 (MQ=255)
ttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCTAGTGCGAGCAATATCAACGTGGtttt > 2:110712/1‑132 (MQ=255)
ttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCTAGTGCGAGCAATATCAACGTGGtttt < 1:110712/132‑1 (MQ=255)
atatCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:1337595/90‑1 (MQ=14)
atatCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 2:1337595/1‑90 (MQ=14)
atCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc < 1:1258170/97‑1 (MQ=255)
atCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc > 2:1258170/1‑97 (MQ=255)
atCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg < 1:252566/139‑1 (MQ=255)
ccTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:463505/84‑1 (MQ=255)
ccTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 1:463505/1‑84 (MQ=11)
tGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcc < 2:1305556/139‑1 (MQ=255)
gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa < 1:590843/93‑1 (MQ=255)
gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa > 2:590843/1‑93 (MQ=255)
gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc < 2:877728/139‑1 (MQ=255)
tGCGAGGGGGGGG‑CTGGAACCGCCACGTGCGTAAGGGCACTAAAACCTGAAGCTAGCGCGTCTACCGATTCCGCCGCCTTCGCCCAGTACTCGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGCGGACTTGAACCCCCa < 2:1156381/139‑1 (MQ=18)
|
CGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCA > NC_000913/4606084‑4606343
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
