Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,627,625 |
T→C |
L37L (TTG→CTG) |
ydfG → |
NADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,627,625 | 0 | T | C | 100.0%
| 264.1
/ NA
| 71 | L37L (TTG→CTG) | ydfG | NADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (35/36); total (35/36) |
GACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATC > NC_000913/1627488‑1627747
|
gACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTC‑ACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTg < 1:17064/139‑1 (MQ=255)
acacaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa > 1:702129/1‑139 (MQ=255)
acaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa < 1:508995/137‑1 (MQ=255)
acaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa > 2:508995/1‑137 (MQ=255)
aaaaGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAg > 1:1695947/1‑139 (MQ=255)
gTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTaaaa < 1:212428/139‑1 (MQ=255)
aGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACg < 2:702129/139‑1 (MQ=255)
aCAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACt > 2:1477221/1‑139 (MQ=255)
cAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTg > 2:1130922/1‑139 (MQ=255)
tGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGga > 1:747282/1‑139 (MQ=255)
gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt < 1:1477221/139‑1 (MQ=255)
tgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt < 2:246371/137‑1 (MQ=255)
tgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt > 1:246371/1‑137 (MQ=255)
atCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt < 1:1369024/135‑1 (MQ=255)
atCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt > 2:1369024/1‑135 (MQ=255)
gTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGt > 1:1707422/1‑139 (MQ=255)
tAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg > 1:788418/1‑104 (MQ=255)
tAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg < 2:788418/104‑1 (MQ=255)
tGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag < 2:522191/119‑1 (MQ=255)
tGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag > 1:522191/1‑119 (MQ=255)
gCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg > 1:537971/1‑139 (MQ=255)
tGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACcgc > 1:1168941/1‑139 (MQ=255)
ggTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGccc > 1:996566/1‑118 (MQ=255)
ggTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGccc < 2:996566/118‑1 (MQ=255)
gTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGCAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc < 1:1130922/139‑1 (MQ=255)
gAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCACCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg < 1:623529/139‑1 (MQ=255)
aTGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt > 1:383947/1‑97 (MQ=255)
aTGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt < 2:383947/97‑1 (MQ=255)
aTGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg < 2:192476/119‑1 (MQ=255)
aTGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg > 1:192476/1‑119 (MQ=255)
aTTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg > 2:672130/1‑133 (MQ=255)
aTTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg < 1:672130/133‑1 (MQ=255)
tACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt > 2:329598/1‑91 (MQ=255)
tACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt < 1:329598/91‑1 (MQ=255)
tcgtcgTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag < 1:292470/86‑1 (MQ=255)
tcgtcgTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag > 2:292470/1‑86 (MQ=255)
gtcgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCACAACCGCGCCGCTATTGAAGAGAt > 2:730748/1‑139 (MQ=255)
cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt > 1:870316/1‑84 (MQ=255)
cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt < 2:870316/84‑1 (MQ=255)
cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACG‑TCGCAACCgcgc < 1:427601/121‑1 (MQ=255)
cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACG‑TCGCAACCgcgc > 2:427601/1‑121 (MQ=255)
gtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGc < 2:1522112/57‑1 (MQ=255)
gtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGc > 1:1522112/1‑57 (MQ=255)
tttATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg > 1:778105/1‑103 (MQ=255)
tttATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg < 2:778105/103‑1 (MQ=255)
ttATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCA‑GAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCa < 1:1214030/139‑1 (MQ=255)
tATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCa < 2:747282/139‑1 (MQ=255)
tATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCa < 2:537971/139‑1 (MQ=255)
caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt < 2:1281955/72‑1 (MQ=255)
caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt > 1:1281955/1‑72 (MQ=255)
gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg < 2:1089726/109‑1 (MQ=255)
gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg > 1:1089726/1‑109 (MQ=255)
gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGcc < 2:1695947/139‑1 (MQ=255)
gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGcc > 1:435698/1‑139 (MQ=255)
ttATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAgtggtg < 2:1719110/139‑1 (MQ=255)
aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGaa > 2:1235665/1‑105 (MQ=255)
aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGaa < 1:1235665/105‑1 (MQ=255)
aCTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag < 2:1719311/49‑1 (MQ=255)
aCTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag > 1:1719311/1‑49 (MQ=255)
aCTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc < 2:1709400/89‑1 (MQ=255)
aCTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc > 1:1709400/1‑89 (MQ=255)
cTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGa > 2:1547726/1‑139 (MQ=255)
tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTgg < 2:1613804/44‑1 (MQ=255)
tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTgg > 1:1613804/1‑44 (MQ=255)
tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt > 1:1473496/1‑49 (MQ=255)
tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt < 2:1473496/49‑1 (MQ=255)
tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg > 2:345773/1‑71 (MQ=255)
tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg > 2:101181/1‑71 (MQ=255)
tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg < 1:345773/71‑1 (MQ=255)
tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg < 1:101181/71‑1 (MQ=255)
ccGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCACAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATc < 1:730748/139‑1 (MQ=255)
|
GACACACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATC > NC_000913/1627488‑1627747
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
