Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,827,351	G→A	320 (TAG→TAA)	srlE →	glucitol/sorbitol‑specific enzyme IIB component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,827,351	0	G	A	100.0%	179.0 / NA	55	320 (TAG→TAA)	srlE	glucitol/sorbitol‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/32); total (23/32)

GTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTA  >  NC_000913/2827217‑2827477

gtcggtTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            >  1:102089/1‑139 (MQ=255)

gtcggtTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            <  1:566812/139‑1 (MQ=255)

           gCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc                                                                                                                 <  2:36358/139‑1 (MQ=255)

           gCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGAc                                                                                                                 <  1:654566/139‑1 (MQ=255)

                    aGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGttattta                                                                                                        <  2:1545117/139‑1 (MQ=255)

                         gTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGa                                                                                                   <  2:442981/139‑1 (MQ=255)

                         gTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGa                                                                                                   <  2:1093603/139‑1 (MQ=255)

                              gACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGaccacc                                                                                              <  2:102089/139‑1 (MQ=255)

                              gACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGaccacc                                                                                              <  1:1454721/139‑1 (MQ=255)

                               acacGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGAccacca                                                                                             <  2:1720613/139‑1 (MQ=255)

                                   ggTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCt                                                                                                                           <  2:195370/105‑1 (MQ=255)

                                   ggTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCt                                                                                                                           >  1:195370/1‑105 (MQ=255)

                                             ggTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGgc                                                                               >  2:1209437/1‑139 (MQ=255)

                                                      tCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            <  1:1065611/86‑1 (MQ=255)

                                                      tCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            >  2:1065611/1‑86 (MQ=255)

                                                           aCTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGa                                                                 >  2:501619/1‑139 (MQ=255)

                                                             tGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            <  2:1263900/78‑1 (MQ=255)

                                                             tGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            >  1:1263900/1‑78 (MQ=255)

                                                             tGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATATATCAGACCACCATCACCCGTATCGGCGCGAg                                                                          >  2:1124401/1‑128 (MQ=255)

                                                             aggTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAg                                                                          <  1:1124401/127‑1 (MQ=255)

                                                               gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            >  1:189894/1‑76 (MQ=255)

                                                               gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGc                                                                                                                            <  2:189894/76‑1 (MQ=255)

                                                               gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGcc                                                             >  2:1723756/1‑139 (MQ=255)

                                                                tGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                                              >  1:685015/1‑137 (MQ=255)

                                                                tGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                                              <  2:685015/137‑1 (MQ=255)

                                                                 ggtttgttttttATCCGGCTCGCCGACTGTAC‑TGATCGCCTGGTGTGTCTCCGGTGTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCt                                                           <  2:16363/132‑1 (MQ=255)

                                                                 gAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCt                                                           <  2:1415188/139‑1 (MQ=255)

                                                                    ccGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCt                                                           <  1:475572/136‑1 (MQ=255)

                                                                    ccGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCt                                                           >  2:475572/1‑136 (MQ=255)

                                                                    ccGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAg                                                        >  2:284504/1‑139 (MQ=255)

                                                                            tAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa                                                <  1:284504/139‑1 (MQ=255)

                                                                            tAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa                                                <  1:1209437/139‑1 (MQ=255)

                                                                                   cgcgcCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcat                                         <  1:501619/139‑1 (MQ=255)

                                                                                                c‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAg                                                 <  2:556161/118‑1 (MQ=255)

                                                                                                c‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAg                                                 >  1:556161/1‑118 (MQ=255)

                                                                                                    aTCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACa                                                                                                                     >  2:1573714/1‑47 (MQ=255)

                                                                                                    aTCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACa                                                                                                                     <  1:1573714/47‑1 (MQ=255)

                                                                                                     tCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcg                        <  1:1382956/139‑1 (MQ=255)

                                                                                                      cGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGaccac                                                                                               <  2:1253992/67‑1 (MQ=255)

                                                                                                      cGCCTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGaccac                                                                                               >  1:1253992/1‑67 (MQ=255)

                                                                                                         cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGa                                                                           >  2:931102/1‑84 (MQ=255)

                                                                                                         cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGa                                                                           <  1:931102/84‑1 (MQ=255)

                                                                                                         cTGGTTTGTCTCCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGcc                    >  1:1301549/1‑139 (MQ=255)

                                                                                                                 tctcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGAcc            <  2:1383185/139‑1 (MQ=255)

                                                                                                                   tcCGGTTTTATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTc          >  2:1370219/1‑139 (MQ=255)

                                                                                                                        ttttATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGac                                                                                                  <  2:1619880/46‑1 (MQ=255)

                                                                                                                        ttttATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGac                                                                                                  >  1:1619880/1‑46 (MQ=255)

                                                                                                                         tttATCTATCAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAagag    <  2:1301549/139‑1 (MQ=255)

                                                                                                                           tatctatcAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTa  >  1:843540/1‑139 (MQ=255)

                                                                                                                                  cAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                                              <  2:1035748/72‑1 (MQ=255)

                                                                                                                                  cAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                                              >  2:1529962/1‑72 (MQ=255)

                                                                                                                                  cAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                                              <  2:1022827/72‑1 (MQ=255)

                                                                                                                                  cAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                                              <  1:1529962/72‑1 (MQ=255)

                                                                                                                                  cAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                                              >  1:1035748/1‑72 (MQ=255)

                                                                                                                                  cAATAAAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGc                                                              >  1:1022827/1‑72 (MQ=255)

GTCGGTTTGTCGCTGGCGGAAGCCCGTCAGGACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTAC‑TGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTA  >  NC_000913/2827217‑2827477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A