Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,538,785	C→T	239 (TAG→TAA)	nanC ←	N‑acetylnuraminic acid outer membrane channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,538,785	0	C	T	100.0%	182.9 / NA	56	239 (TAG→TAA)	nanC	N‑acetylnuraminic acid outer membrane channel protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/28); total (28/28)

GTCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTACTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTC  >  NC_000913/4538649‑4538902

gtCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTctatta                                                                                                                     >  2:876700/1‑139 (MQ=255)

       tCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTa                                                                                                              <  1:489985/139‑1 (MQ=255)

            tGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGa                                                                                                         <  1:1092102/139‑1 (MQ=255)

              cTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGaca                                                                                                       >  2:627849/1‑139 (MQ=255)

                   gTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCa                                                                                                  >  1:726156/1‑139 (MQ=255)

                         cTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGa                                                                                            >  2:265363/1‑139 (MQ=255)

                                    cacaGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTc                                                                                 >  2:639759/1‑139 (MQ=255)

                                      caGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCg                                                                               >  2:450067/1‑139 (MQ=255)

                                              tCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTa                                                                       >  1:1267088/1‑139 (MQ=255)

                                                    aaTACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                 <  2:726156/139‑1 (MQ=255)

                                                    aaTACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                 <  1:876700/139‑1 (MQ=255)

                                                    aaTACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTg                                                                 <  2:1267088/139‑1 (MQ=255)

                                                         aGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCg                                                              >  2:1572968/1‑137 (MQ=255)

                                                         aGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCg                                                              <  1:1572968/137‑1 (MQ=255)

                                                           aCGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGt                                                             >  2:1292861/1‑136 (MQ=255)

                                                           aCGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGt                                                             <  1:1292861/136‑1 (MQ=255)

                                                            cgcgTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTa                                                         >  1:1232395/1‑139 (MQ=255)

                                                                tttGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTTTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTcc                                                                                >  2:1446752/1‑112 (MQ=255)

                                                                tttGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTTTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTcc                                                                                <  1:1446752/112‑1 (MQ=255)

                                                                tttGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCaa                                                                                                 >  2:25487/1‑95 (MQ=255)

                                                                tttGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCaa                                                                                                 <  1:25487/95‑1 (MQ=255)

                                                                tttGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGtt                                                                                    <  1:1156096/108‑1 (MQ=255)

                                                                tttGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGAAACACCAATGCGATAACTGtt                                                                                    >  2:1156096/1‑108 (MQ=255)

                                                                  tGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACAcc                                                   <  1:639759/139‑1 (MQ=255)

                                                                                 catcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTa                                    >  2:1456336/1‑139 (MQ=255)

                                                                                   tcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCa                                         <  1:1217478/132‑1 (MQ=255)

                                                                                   tcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCa                                         >  2:1217478/1‑132 (MQ=255)

                                                                                   tcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGt                                  <  1:526649/139‑1 (MQ=255)

                                                                                   tcatGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGt                                  >  2:526649/1‑139 (MQ=255)

                                                                                    catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTcc                                                                                <  1:605138/92‑1 (MQ=255)

                                                                                    catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTcc                                                                                >  2:605138/1‑92 (MQ=255)

                                                                                    catGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                 <  1:308146/139‑1 (MQ=255)

                                                                                      tGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCat                               <  1:627849/139‑1 (MQ=255)

                                                                                            cAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCaa                                                                                                 <  1:408739/67‑1 (MQ=255)

                                                                                            cAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCaa                                                                                                 >  2:408739/1‑67 (MQ=255)

                                                                                            cAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGa                                                                              >  2:1251406/1‑86 (MQ=255)

                                                                                            cAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGa                                                                              <  1:1251406/86‑1 (MQ=255)

                                                                                              aGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCtatg                       >  1:1507231/1‑139 (MQ=255)

                                                                                               gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAAt                                                                         >  1:1598450/1‑88 (MQ=255)

                                                                                               gcgcCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAAt                                                                         <  2:1598450/88‑1 (MQ=255)

                                                                                                             ttATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCg                                                                               <  1:1190713/68‑1 (MQ=255)

                                                                                                             ttATTCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCg                                                                               >  2:1190713/1‑68 (MQ=255)

                                                                                                                ttCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGc     >  1:1016906/1‑139 (MQ=255)

                                                                                                                 tCATTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCg    <  1:1456336/139‑1 (MQ=255)

                                                                                                                   aTTGTGACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTc  <  1:265363/139‑1 (MQ=255)

                                                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                <  2:546497/91‑1 (MQ=255)

                                                                                                                     tgtgACTGTCTCCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTg                                                >  1:546497/1‑91 (MQ=255)

                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCa                                         >  2:598223/1‑90 (MQ=255)

                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCa                                         <  1:598223/90‑1 (MQ=255)

                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                      >  2:336299/1‑93 (MQ=255)

                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                      <  1:1360005/93‑1 (MQ=255)

                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                      >  2:1360005/1‑93 (MQ=255)

                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                      <  1:336299/93‑1 (MQ=255)

                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                 >  2:1597473/1‑98 (MQ=255)

                                                                                                                             tctcCTGTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTc                                 <  1:1597473/98‑1 (MQ=255)

                                                                                                                                   gTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                      <  2:1284474/87‑1 (MQ=255)

                                                                                                                                   gTCTATTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAgg                                      >  1:1284474/1‑87 (MQ=255)

GTCGTTATCAATTGCTCCGGTACCACTTTTAAATGGCACAGGAGTTTCCGGTAATACAGACGCGTTTGCGGCAAATGAAGCCATCATGATAGCAAGCGCCGTTATTGTTTTATTCATTGTGACTGTCTCCTGTCTACTACAGTTTAAATGACACACCAATGCGATAACTGTTTTCCGATAAATTATCTCTGCCGTTGTAAACACCCTGACGGTCAAGGTAGTCATATTCTATGTATGGCGTAATATCGGGCGTC  >  NC_000913/4538649‑4538902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A