Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	825,102	G→A	A336V (GCG→GTG)	ybhR ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	825,102	0	G	A	100.0%	150.9 / NA	47	A336V (GCG→GTG)	ybhR	putative ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/20); total (27/20)

TGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCAT  >  NC_000913/824985‑825235

tGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCt                                                                                                                  >  1:310044/1‑139 (MQ=255)

   cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTgg                                                                                                               >  2:795261/1‑139 (MQ=255)

                        aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                          <  1:800533/139‑1 (MQ=255)

                        aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                          >  2:361532/1‑139 (MQ=255)

                         ccTTACGTCTAAACATCGCGTACGCCGCTGACCTTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                         <  1:270800/139‑1 (MQ=255)

                         ccTTACGTCTAAACATCGCGTACGCCGCTGACCTTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                         >  2:270800/1‑139 (MQ=255)

                           ttACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                          <  1:828352/136‑1 (MQ=255)

                           ttACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                          >  2:828352/1‑136 (MQ=255)

                                    aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc                                                                              >  2:121501/1‑139 (MQ=255)

                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCt                                                                                                                     <  1:224824/95‑1 (MQ=255)

                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCt                                                                                                                     >  2:224824/1‑95 (MQ=255)

                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAAt                                                                                                           <  1:209976/105‑1 (MQ=255)

                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAAt                                                                                                           >  2:209976/1‑105 (MQ=255)

                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                          <  2:914405/122‑1 (MQ=255)

                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                          >  1:914405/1‑122 (MQ=255)

                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTc                                                                         >  2:638536/1‑139 (MQ=255)

                                         cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTc                                                                         <  1:807776/139‑1 (MQ=255)

                                           cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGt                                                                                                              <  1:901297/100‑1 (MQ=255)

                                           cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGt                                                                                                              >  2:901297/1‑100 (MQ=255)

                                           cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTaa                                                                                                            >  1:930096/1‑102 (MQ=255)

                                           cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTaa                                                                                                            <  2:930096/102‑1 (MQ=255)

                                                 ccgcTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTg                                                                 <  1:361532/139‑1 (MQ=255)

                                                  cgcTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGc                                                                <  1:121501/139‑1 (MQ=255)

                                                    cTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAg                                                              >  1:669519/1‑139 (MQ=255)

                                                            gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCg                                                      >  1:253552/1‑139 (MQ=255)

                                                              cGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGc                                                    <  1:638536/139‑1 (MQ=255)

                                                                       tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaa                                                                                                   <  2:922243/83‑1 (MQ=255)

                                                                       tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaa                                                                                                   >  1:922243/1‑83 (MQ=255)

                                                                       tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTcc                                           >  1:146634/1‑139 (MQ=255)

                                                                         tCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCAc                                         <  2:186883/139‑1 (MQ=255)

                                                                           aCCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCACACCGGCATGTTTTCCACCg                                       >  1:604851/1‑139 (MQ=255)

                                                                             cAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGga                                     <  2:146634/139‑1 (MQ=255)

                                                                                tAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTACAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGaa                                  >  1:369120/1‑139 (MQ=255)

                                                                                  gCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAAc                                >  1:796325/1‑139 (MQ=255)

                                                                                   cGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTaa                                                                                                            <  2:153560/62‑1 (MQ=255)

                                                                                   cGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTaa                                                                                                            >  1:153560/1‑62 (MQ=255)

                                                                                     gCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGc                                                    >  1:921424/1‑116 (MQ=255)

                                                                                     gCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGc                                                    <  2:921424/116‑1 (MQ=255)

                                                                                         caAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCg                         <  2:669519/139‑1 (MQ=255)

                                                                                           aaCTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                       <  1:795261/139‑1 (MQ=255)

                                                                                                ttCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGga                  >  1:796713/1‑139 (MQ=255)

                                                                                                     cacaATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGAAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATg             >  2:454958/1‑139 (MQ=255)

                                                                                                          tgtCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAAc                           >  2:558103/3‑120 (MQ=255)

                                                                                                          tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAAc                           <  1:558103/120‑1 (MQ=255)

                                                                                                          tatCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCggg        >  2:516533/1‑139 (MQ=255)

                                                                                                             ccAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGcat     >  1:738781/1‑139 (MQ=255)

                                                                                                                gACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGcatcat  >  1:741581/1‑139 (MQ=255)

TGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCAT  >  NC_000913/824985‑825235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A