Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	825,102	G→A	A336V (GCG→GTG)	ybhR ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	825,102	0	G	A	100.0%	98.1 / NA	32	A336V (GCG→GTG)	ybhR	putative ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/16); total (16/16)

TTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAA  >  NC_000913/824980‑825237

tttGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                         <  2:789076/139‑1 (MQ=255)

tttGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                         <  1:448988/139‑1 (MQ=255)

        cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCCGACCCTGTCGTGGCCCTTATCACCAGTAGCGGCCACAAACTATTCCACATAATATCCAGACTCAACTCATTCAACTAAATCCGCTTgg                                                                                                                 >  1:111889/1‑139 (MQ=255)

         gAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGt                                                                                                                <  1:293663/139‑1 (MQ=255)

                     gTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                                                                    <  2:440269/139‑1 (MQ=255)

                        aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                                 >  1:361387/1‑139 (MQ=255)

                              ccTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                           <  2:67568/139‑1 (MQ=255)

                                   cGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                            >  1:110366/1‑133 (MQ=255)

                                   cGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                            <  2:110366/133‑1 (MQ=255)

                                         aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc                                                                                >  2:703842/1‑139 (MQ=255)

                                               gcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCAGACAATATCCAGACTCACATCCTGCAAATAAATATGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcaaagtaa                                                                          >  1:513605/1‑132 (MQ=255)

                                                cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAg                                                                         >  1:232577/1‑139 (MQ=255)

                                                    cgccgcTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGttt                                                                     <  2:227098/139‑1 (MQ=255)

                                                     gccgcTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtttt                                                                    <  2:242731/139‑1 (MQ=255)

                                                     gccgcTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtttt                                                                    <  1:566326/139‑1 (MQ=255)

                                                          tGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                           <  1:569109/111‑1 (MQ=255)

                                                          tGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                           >  2:569109/1‑111 (MQ=255)

                                                                      ggCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATg                                                   >  2:716381/1‑139 (MQ=255)

                                                                                 ccAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCgg                                        <  2:361387/139‑1 (MQ=255)

                                                                                         ggCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGt                                <  2:232577/139‑1 (MQ=255)

                                                                                          gCCACAAACTATTCCACACAATATCCAGACTCACATCCt                                                                                                                                   >  1:610399/1‑39 (MQ=255)

                                                                                          gCCACAAACTATTCCACACAATATCCAGACTCACATCCt                                                                                                                                   <  2:610399/39‑1 (MQ=255)

                                                                                               aaaCTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                                                                    <  2:131456/65‑1 (MQ=255)

                                                                                               aaaCTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTaaa                                                                                                    >  1:131456/1‑65 (MQ=255)

                                                                                                 aCTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGaa                        <  1:690427/139‑1 (MQ=255)

                                                                                                     ttCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGga                    >  1:542421/1‑139 (MQ=255)

                                                                                                             aaTATCCAGACTCACATCCTTCAACTAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCg            >  2:561163/1‑139 (MQ=255)

                                                                                                                      aCTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGTAGAAACGTAACCGGa                         <  1:537039/117‑1 (MQ=255)

                                                                                                                      aCTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATa   >  2:713945/1‑139 (MQ=255)

                                                                                                                      aCTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGAGGGCATCATa   >  1:371779/1‑139 (MQ=255)

                                                                                                                      aCTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGAGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCAGCGGAGAAACGTAACCGGa                         >  2:537039/1‑117 (MQ=255)

                                                                                                                       cTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTACACGAAAAGGAGAATGGAGGGCATCATaa  >  1:184162/1‑139 (MQ=255)

TTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAA  >  NC_000913/824980‑825237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A