Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,382,141 |
A→G |
R65R (AGA→AGG) |
ompG → |
outer membrane porin G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,382,141 | 0 | A | G | 100.0%
| 161.0
/ NA
| 45 | R65R (AGA→AGG) | ompG | outer membrane porin G |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/23); total (22/23) |
AAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACGATGATTTCAG > NC_000913/1382014‑1382260
|
aaaggaaCGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc < 1:402939/139‑1 (MQ=255)
aaggaaCGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGcc < 1:193820/139‑1 (MQ=255)
aCTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCa > 2:375320/1‑139 (MQ=255)
tttAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTGTCAGGAAGGGc < 1:826565/139‑1 (MQ=255)
tttAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGc < 2:332001/139‑1 (MQ=255)
tttAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGc < 2:456899/139‑1 (MQ=255)
tCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGa > 2:889506/1‑139 (MQ=255)
gcgcGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc > 1:18760/1‑112 (MQ=255)
gcgcGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc < 2:18760/112‑1 (MQ=255)
aGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCTGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgt > 2:56673/1‑139 (MQ=255)
gTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc > 2:69510/1‑89 (MQ=255)
gTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc < 1:69510/89‑1 (MQ=255)
tCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGct < 2:419331/83‑1 (MQ=255)
tCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGct > 1:419331/1‑83 (MQ=255)
aGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGct < 1:26784/80‑1 (MQ=255)
aGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGct > 2:26784/1‑80 (MQ=255)
aGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCt > 1:241595/1‑74 (MQ=255)
aGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCt < 2:241595/74‑1 (MQ=255)
aGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAg < 1:889506/139‑1 (MQ=255)
gATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGc < 2:530353/139‑1 (MQ=255)
ggatggGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAg > 1:413805/1‑139 (MQ=255)
gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCg > 2:154641/1‑79 (MQ=255)
gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCg < 1:154641/79‑1 (MQ=255)
gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCAt > 1:214806/1‑139 (MQ=255)
gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCAt > 2:528881/1‑139 (MQ=255)
gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCAt > 2:30019/1‑139 (MQ=255)
cTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGa > 2:252443/1‑131 (MQ=255)
cTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGa < 1:252443/131‑1 (MQ=255)
cTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCAtt > 2:838718/1‑139 (MQ=255)
gCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAg > 1:56337/1‑86 (MQ=255)
gCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAg < 2:56337/86‑1 (MQ=255)
cGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc > 1:757335/1‑55 (MQ=255)
cGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc < 2:757335/55‑1 (MQ=255)
ccTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGcc < 1:137111/112‑1 (MQ=255)
ccTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGcc > 2:137111/1‑112 (MQ=255)
tATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc < 1:549452/41‑1 (MQ=255)
tATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc > 2:549452/1‑41 (MQ=255)
ttttAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACgat < 2:413805/139‑1 (MQ=255)
aTGCCGCCAACGGGCGGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGAGGGTAAAAGTGGAGCGGGGTTTGTTCGCCCGGAGCTGGAGGTGCAGTATCACTTCCTCGAAAACGATGAttt < 2:851750/139‑1 (MQ=255)
aTGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACGATGAttt < 1:375320/139‑1 (MQ=255)
ccgccAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACGATGATTTCAg > 1:535902/1‑139 (MQ=255)
gccAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTgg > 1:243418/1‑100 (MQ=255)
gccAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTgg < 2:243418/100‑1 (MQ=255)
cGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGcgcg < 1:183404/53‑1 (MQ=255)
cGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGcgcg > 2:183404/1‑53 (MQ=255)
|
AAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACGATGATTTCAG > NC_000913/1382014‑1382260
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
