Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,006,526	A→G	D124G (GAC→GGC)	amyA →	cytoplasmic alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,006,526	0	A	G	100.0%	136.0 / NA	39	D124G (GAC→GGC)	amyA	cytoplasmic alpha‑amylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/21); total (18/21)

CTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACC  >  NC_000913/2006402‑2006660

ctgctgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTg                                                                                                                          <  2:27008/139‑1 (MQ=255)

  gctgGCCGCCATTGATGCTCTGAAATGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGAc                                                                                                                        <  1:302608/139‑1 (MQ=255)

    tgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACga                                                                                                                      <  2:164837/139‑1 (MQ=255)

             ttGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCAt                                                                                                             >  2:863850/1‑139 (MQ=255)

               gATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTg                                                                                                           <  2:37587/139‑1 (MQ=255)

                              aaTGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCg                                                                                                                                     >  2:285440/1‑98 (MQ=255)

                              aaTGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCg                                                                                                                                     <  1:285440/98‑1 (MQ=255)

                              aaTGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGa                                                                                            <  1:475668/139‑1 (MQ=255)

                                tGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGAcg                                                                                          >  2:108601/1‑139 (MQ=255)

                                      tGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTac                                                                                    >  1:367555/1‑139 (MQ=255)

                                                 tGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCcccgc                                                                         <  2:367555/139‑1 (MQ=255)

                                                        ggTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGcc                                                                  <  2:366123/139‑1 (MQ=255)

                                                         gTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCg                                                                 >  2:714148/1‑139 (MQ=255)

                                                          tAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaagaa                                                                                                                  <  2:527376/89‑1 (MQ=255)

                                                          tAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaagaa                                                                                                                  >  1:527376/1‑89 (MQ=255)

                                                             tCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCa                                                             >  1:397107/1‑139 (MQ=255)

                                                                  cacaAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACt                                                        <  2:544940/139‑1 (MQ=255)

                                                                   acaAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTc                                                       <  1:108601/139‑1 (MQ=255)

                                                                    caAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCAtt                                                                                                            <  2:13560/85‑1 (MQ=255)

                                                                    caAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCAtt                                                                                                            >  1:13560/1‑85 (MQ=255)

                                                                    caAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCg                                                      >  1:759355/1‑139 (MQ=255)

                                                                      aaaTGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCa                                                    <  2:759355/139‑1 (MQ=255)

                                                                          gggCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCggg                                                               <  1:646653/124‑1 (MQ=255)

                                                                          gggCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCggg                                                               >  2:646653/1‑124 (MQ=255)

                                                                                            agCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGt                              >  2:118592/1‑139 (MQ=255)

                                                                                            agCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGt                              >  2:37452/1‑139 (MQ=255)

                                                                                            agCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGt                              >  1:34464/1‑139 (MQ=255)

                                                                                                aTTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTa                          <  1:118592/139‑1 (MQ=255)

                                                                                                aTTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTa                          <  1:37452/139‑1 (MQ=255)

                                                                                                 ttCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAg                         <  1:733767/139‑1 (MQ=255)

                                                                                                 ttCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAg                         <  1:863850/139‑1 (MQ=255)

                                                                                                    gcgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCaa                                  <  1:74780/127‑1 (MQ=255)

                                                                                                    gcgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCaa                                  >  2:74780/1‑127 (MQ=255)

                                                                                                     cgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGt                     <  1:714148/139‑1 (MQ=255)

                                                                                                     cgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGt                     <  2:397107/139‑1 (MQ=255)

                                                                                                       tGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTAt                   >  2:72232/1‑139 (MQ=255)

                                                                                                              tgtAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACAat            >  2:207051/1‑139 (MQ=255)

                                                                                                                    tGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGaa      >  2:59228/1‑139 (MQ=255)

                                                                                                                      cTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTAACTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGaaaa    >  2:614236/1‑139 (MQ=255)

                                                                                                                        gatggCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTaca                                                                                   <  2:200383/58‑1 (MQ=255)

                                                                                                                        gatggCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTaca                                                                                   >  1:200383/1‑58 (MQ=255)

                                                                                                                        gatggCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAAcc  >  2:757502/1‑139 (MQ=255)

CTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACC  >  NC_000913/2006402‑2006660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A