Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I2 R1 780 68.3 3343050 93.5% 3125751 102.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,006,526 A→G D124G (GAC→GGC)  amyA → cytoplasmic alpha‑amylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,006,5260AG97.9% 335.3 / ‑3.1 97D124G (GAC→GGC) amyAcytoplasmic alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (47/48);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

Only 100 of 103 total aligned reads displayed.

ACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACC  >  NC_000913/2006398‑2006660
                                                                                                                                |                                                                                                                                      
acaACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCaa                                                                                                                              >  1:185187/1‑139 (MQ=255)
    ctgctgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTg                                                                                                                          <  2:1289592/139‑1 (MQ=255)
    ctgctgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTg                                                                                                                          >  2:724867/1‑139 (MQ=255)
       ctgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACg                                                                                                                       >  2:157394/1‑139 (MQ=255)
        tgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACga                                                                                                                      <  1:157394/139‑1 (MQ=255)
        tgGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACga                                                                                                                      <  1:1461440/139‑1 (MQ=255)
         ggCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaa                                                                                                                     >  1:902568/1‑139 (MQ=255)
            cgccATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaagaa                                                                                                                  >  2:710998/1‑139 (MQ=255)
                 ttGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCAt                                                                                                             <  1:331449/139‑1 (MQ=255)
                          tGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCa                                                                                                              <  2:1482550/129‑1 (MQ=255)
                          tGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCa                                                                                                              >  1:1482550/1‑129 (MQ=255)
                           gAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGaa                                                                                                   >  2:1164165/1‑139 (MQ=255)
                           gAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGaa                                                                                                   >  2:1657745/1‑139 (MQ=255)
                            aaaCGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaag                                                                                                                    <  2:925267/121‑1 (MQ=255)
                            aaaCGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaag                                                                                                                    >  1:925267/1‑121 (MQ=255)
                            aaaCGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTg                                                                                                           <  2:1233393/130‑1 (MQ=255)
                            aaaCGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTg                                                                                                           >  1:1233393/1‑130 (MQ=255)
                                  aaTGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGa                                                                                            <  1:334349/139‑1 (MQ=255)
                                      aCATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGAcgcg                                                                                        >  2:1528589/1‑139 (MQ=255)
                                         ttGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTa                                                                                     <  2:842944/139‑1 (MQ=255)
                                          tGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTac                                                                                    >  1:776100/1‑139 (MQ=255)
                                            cGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTacac                                                                                  >  1:839294/1‑139 (MQ=255)
                                            cGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTacac                                                                                  >  2:1446838/1‑139 (MQ=255)
                                                    ttGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCAtt                                                                                                            <  2:562154/105‑1 (MQ=255)
                                                    ttGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCAtt                                                                                                            >  1:562154/1‑105 (MQ=255)
                                                     tGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCcccgc                                                                         <  2:776100/139‑1 (MQ=255)
                                                       gATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCcccgccc                                                                       <  1:710998/139‑1 (MQ=255)
                                                         tgtgGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGa                                                                                                                         >  2:358477/1‑87 (MQ=255)
                                                         tgtgGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGa                                                                                                                         <  1:358477/87‑1 (MQ=255)
                                                           tgGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTa                                                                                                                                   >  1:1533557/1‑75 (MQ=255)
                                                           tgGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTa                                                                                                                                   <  2:1533557/75‑1 (MQ=255)
                                                            ggTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGcc                                                                  <  1:1164165/139‑1 (MQ=255)
                                                              tAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaag                                                                                                                    <  1:829692/87‑1 (MQ=255)
                                                              tAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaag                                                                                                                    >  2:829692/1‑87 (MQ=255)
                                                                 tCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCcccgc                                                                         <  2:281233/127‑1 (MQ=255)
                                                                 tCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCcccgc                                                                         >  1:281233/1‑127 (MQ=255)
                                                                 tCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCa                                                             >  2:516996/1‑139 (MQ=255)
                                                                   aaCCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGAc                                                                                                                        >  2:141918/1‑78 (MQ=255)
                                                                   aaCCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGAc                                                                                                                        <  1:141918/78‑1 (MQ=255)
                                                                   aaCCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGtgtg                                                                                                     >  2:1583053/1‑97 (MQ=255)
                                                                   aaCCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGtgtg                                                                                                     <  1:1583053/97‑1 (MQ=255)
                                                                     ccACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGc                                                                   >  2:1611193/1‑129 (MQ=255)
                                                                     ccACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGc                                                                   <  1:1611193/129‑1 (MQ=255)
                                                                     ccACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATAc                                                         >  1:223161/1‑139 (MQ=255)
                                                                      cacaAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCcccgc                                                                         >  1:876155/1‑122 (MQ=255)
                                                                      cacaAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCcccgc                                                                         <  2:876155/122‑1 (MQ=255)
                                                                      cacaAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACt                                                        <  1:1657745/139‑1 (MQ=255)
                                                                        caAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACg                                                                                                                                 >  1:1396144/1‑64 (MQ=255)
                                                                        caAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACg                                                                                                                                 <  2:1396144/64‑1 (MQ=255)
                                                                        caAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGt                                                                                       >  1:522391/1‑106 (MQ=255)
                                                                        caAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGt                                                                                       <  2:522391/106‑1 (MQ=255)
                                                                        caAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCg                                                      <  2:185187/139‑1 (MQ=255)
                                                                        caAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCg                                                      >  1:1029972/1‑139 (MQ=255)
                                                                          aaaTGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCa                                                    <  2:902568/139‑1 (MQ=255)
                                                                               ggCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTaca                                                                                   >  1:1022724/1‑103 (MQ=255)
                                                                               ggCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTaca                                                                                   <  2:1022724/103‑1 (MQ=255)
                                                                                gcgcGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACt                                                        <  1:1486224/129‑1 (MQ=255)
                                                                                 cgcgGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTaca                                                                                   <  2:121839/101‑1 (MQ=255)
                                                                                 cgcgGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTaca                                                                                   >  1:121839/1‑101 (MQ=255)
                                                                                   cgGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAAt                                                           >  2:1113357/1‑123 (MQ=255)
                                                                                   cgGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAAt                                                           <  1:1113357/123‑1 (MQ=255)
                                                                                     gATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTggg                                         >  1:600298/1‑139 (MQ=255)
                                                                                             agaagCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCaaa                                 <  2:1107377/139‑1 (MQ=255)
                                                                                             agaagCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCaaa                                 <  1:516996/139‑1 (MQ=255)
                                                                                             agaagCTATTCGCGGGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCaaa                                 <  2:1029972/139‑1 (MQ=255)
                                                                                                agCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATCCTCGCa                                                    >  2:1000314/1‑117 (MQ=255)
                                                                                                agCTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCa                                                    <  1:1000314/117‑1 (MQ=255)
                                                                                                  cTATTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGttt                            <  2:600298/139‑1 (MQ=255)
                                                                                                    aTTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCaa                                  <  1:894512/131‑1 (MQ=255)
                                                                                                    aTTCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCaa                                  >  2:894512/1‑131 (MQ=255)
                                                                                                     ttCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGc                                                              <  1:1503940/102‑1 (MQ=255)
                                                                                                     ttCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGc                                                              >  2:1503940/1‑102 (MQ=255)
                                                                                                     ttCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAg                         <  1:1528589/139‑1 (MQ=255)
                                                                                                     ttCGCGTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAg                         <  2:863367/139‑1 (MQ=255)
                                                                                                       cgcgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaag                                                                                                                    >  1:207995/1‑46 (MQ=255)
                                                                                                       cgcgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACgaag                                                                                                                    <  2:207995/46‑1 (MQ=255)
                                                                                                       cgcgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCaaa                                 >  1:237587/1‑129 (MQ=255)
                                                                                                       cgcgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCaaa                                 <  2:237587/129‑1 (MQ=255)
                                                                                                       cgcgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTAAACCTTCCCAGCACGTGCCGGGCAATACTCGCAGTTTATATGGGAATTCAAATGTTTTAGCg                       >  1:1107377/1‑139 (MQ=255)
                                                                                                         cgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTg                                                                                                           <  1:129487/53‑1 (MQ=255)
                                                                                                         cgTGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTg                                                                                                           >  2:129487/1‑53 (MQ=255)
                                                                                                           tGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGAc                                                                                                                        <  2:1552500/38‑1 (MQ=255)
                                                                                                           tGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGAc                                                                                                                        >  1:1552500/1‑38 (MQ=255)
                                                                                                           tGCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTAt                   <  2:223161/139‑1 (MQ=255)
                                                                                                            gCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGAcg                                                                                          <  1:563393/67‑1 (MQ=255)
                                                                                                            gCAGCGTGTAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGAcg                                                                                          >  2:563393/1‑67 (MQ=255)
                                                                                                            gCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGt                                                  >  1:1178858/1‑107 (MQ=255)
                                                                                                            gCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGt                                                  <  2:1178858/107‑1 (MQ=255)
                                                                                                                  tgtAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCat            >  1:874060/1‑139 (MQ=255)
                                                                                                                   gtAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCata           <  2:839294/139‑1 (MQ=255)
                                                                                                                    tAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGa                                                                                            >  1:181371/1‑57 (MQ=255)
                                                                                                                    tAAATGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGa                                                                                            <  2:181371/57‑1 (MQ=255)
                                                                                                                        tGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGCAGGCTGGAc                                                                                           <  2:1121177/54‑1 (MQ=39)
                                                                                                                        tGCTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGAc                                                                                           >  1:1121177/1‑54 (MQ=255)
                                                                                                                          cTGATGGCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGaaaa    >  1:493735/1‑139 (MQ=255)
                                                                                                                            gatggCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCg                                                      >  1:1661160/1‑87 (MQ=255)
                                                                                                                            gatggCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGAcc              >  2:411599/1‑127 (MQ=255)
                                                                                                                            gatggCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAAcc  >  2:1355813/1‑139 (MQ=255)
                                                                                                                            gatggCCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAAcc  >  1:864118/1‑139 (MQ=255)
                                                                                                                            gatggCCGTACGCAAAGTGACGAAGAAATCATTGAGTGGGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCg                                                      <  2:1661160/87‑1 (MQ=255)
                                                                                                                                |                                                                                                                                      
ACAACTGCTGGCCGCCATTGATGCTCTGAAACGTAATGACATTGCGGTGCTGTTGGATGTGGTAGTCAACCACAAAATGGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTGAGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCCCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTTAGCGGTATCGACCATATCGAAAACC  >  NC_000913/2006398‑2006660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A