Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,186,666	(C)_5→6	coding (750/825 nt)	rcnA →	membrane protein conferring nickel and cobalt resistance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,186,661	1	.	C	100.0%	158.5 / NA	44	A249P (GCC→CCC)	rcnA	membrane protein conferring nickel and cobalt resistance
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (18/26); total (18/26)

GACACTGGGCGCAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATCTTCAGCCCAGGA  >  NC_000913/2186525‑2186780

gACACTGGGCGCAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGTAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑cc                                                                                                                       <  2:769808/139‑1 (MQ=255)

  cacTGGGCGCAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑cccc                                                                                                                     <  2:470418/139‑1 (MQ=255)

      gggCGCAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑ccccccta                                                                                                                 <  1:214380/139‑4 (MQ=255)

       ggCGCAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑cccccctat                                                                                                                >  1:726249/1‑135 (MQ=255)

        gcgcAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑cccccctatt                                                                                                               >  2:783220/1‑134 (MQ=255)

         cgcAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTAttt                                                                                                               >  1:485762/1‑139 (MQ=255)

                  ggTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCt                                                                                                      >  2:56512/1‑139 (MQ=255)

                      gtcAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTg                                                                                                  >  2:519429/1‑139 (MQ=255)

                        cAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGAt                                                                                                >  2:887121/1‑139 (MQ=255)

                               aGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTa                                                                                         <  1:887121/139‑1 (MQ=255)

                                gCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAg                                                                                        <  2:169470/139‑1 (MQ=255)

                                 cATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       <  1:519429/139‑1 (MQ=255)

                                 cATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       <  2:485762/139‑1 (MQ=255)

                                   ttGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCg                                                                                     <  1:575051/139‑1 (MQ=255)

                                   ttGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCg                                                                                     <  2:484532/139‑1 (MQ=255)

                                   ttGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCg                                                                                     <  1:150262/139‑1 (MQ=255)

                                     ggCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGGTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                                                   >  2:575903/1‑139 (MQ=255)

                                     ggCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                                                   <  2:898937/139‑1 (MQ=255)

                                     ggCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                                                   <  1:275750/139‑1 (MQ=255)

                                     ggCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                                                   <  1:850881/139‑1 (MQ=255)

                                     ggCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                                                   <  2:364508/139‑1 (MQ=255)

                                            cGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGGTAAACGCGCCCCCCTATTTTTCCAGACTGTTGATTGGCTTAGTCGGTGTGtata                                                                            <  1:575903/139‑1 (MQ=255)

                                            cGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGtata                                                                            <  1:56512/139‑1 (MQ=255)

                                                        tCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATg                                                                <  2:726249/139‑1 (MQ=255)

                                                               aGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                                                   <  2:382068/113‑1 (MQ=255)

                                                               aGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                                                   >  1:382068/1‑113 (MQ=255)

                                                                 gCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATgg                                                       >  2:871189/1‑139 (MQ=255)

                                                                  cGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATggg                                                      <  1:393974/139‑1 (MQ=255)

                                                                   gTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       >  2:24985/1‑105 (MQ=255)

                                                                   gTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       <  1:24985/105‑1 (MQ=255)

                                                                            gcaATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGa                                            <  1:871189/139‑1 (MQ=255)

                                                                                      ttCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATc                                  >  1:833836/1‑139 (MQ=255)

                                                                                       tCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATcc                                 >  2:609267/1‑139 (MQ=255)

                                                                                         agcagGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCaa                                    >  1:632404/1‑134 (MQ=255)

                                                                                         agcagGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCaa                                    <  2:632404/134‑1 (MQ=255)

                                                                                              gTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTAc                                                                   <  2:355681/98‑1 (MQ=255)

                                                                                              gTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTAc                                                                   >  1:355681/1‑98 (MQ=255)

                                                                                              gTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGc                          >  2:508434/1‑139 (MQ=255)

                                                                                                cGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       <  1:445679/76‑1 (MQ=255)

                                                                                                cGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       >  2:445679/1‑76 (MQ=255)

                                                                                                cGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                                                   <  2:505442/80‑1 (MQ=255)

                                                                                                cGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGgt                                                                                   >  1:505442/1‑80 (MQ=255)

                                                                                                                      aaCACTCTCGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATCTTCAGCCCAGga  <  1:783220/139‑1 (MQ=255)

                                                                                                                            ctcGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATCTTCAGCCCAGga  <  2:298306/133‑1 (MQ=255)

                                                                                                                            ctcGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATCTTCAGCCCAGga  >  1:298306/1‑133 (MQ=255)

                                                                                                                             tcGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       >  1:283619/1‑47 (MQ=255)

                                                                                                                             tcGCTAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       <  2:283619/47‑1 (MQ=255)

                                                                                                                                 tAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       <  1:93285/43‑1 (MQ=255)

                                                                                                                                 tAAACGCGCCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGt                                                                                       >  2:93285/1‑43 (MQ=255)

GACACTGGGCGCAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCG‑CCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATCTTCAGCCCAGGA  >  NC_000913/2186525‑2186780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A