Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,263,478	T→C	V6V (GTA→GTG)	fruB ←	fused fructose‑specific PTS enzymes: IIA component/HPr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,263,478	0	T	C	100.0%	114.6 / NA	32	V6V (GTA→GTG)	fruB	fused fructose‑specific PTS enzymes: IIA component/HPr component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/20); total (12/20)

GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAA  >  NC_000913/2263346‑2263599

ggTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCCTTTCGCCCGGATGGATGTCCTGCACGGAt                                                                                                                     <  2:841450/139‑1 (MQ=255)

  tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATaa                                                                                                                   <  1:847098/139‑1 (MQ=255)

                gcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTtc                                                                                                     <  2:120137/139‑1 (MQ=255)

                     cATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTctcctc                                                                                                >  2:921995/1‑139 (MQ=255)

                                cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt                                                                                     <  1:921995/139‑1 (MQ=255)

                                         cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                                                                                         <  2:482270/126‑1 (MQ=255)

                                         cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                                                                                         >  1:482270/1‑126 (MQ=255)

                                                aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCct                                                                     <  1:776664/139‑1 (MQ=255)

                                                    ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTg                                                                                           >  2:651299/1‑113 (MQ=255)

                                                    ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTg                                                                                           <  1:651299/113‑1 (MQ=255)

                                                    ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTAt                                                                 >  1:123192/1‑139 (MQ=255)

                                                    ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTAt                                                                 >  2:155743/1‑139 (MQ=255)

                                                      ggCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATga                                                               >  1:411654/1‑139 (MQ=255)

                                                            cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGTCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAtt                                                                           <  1:72041/121‑1 (MQ=255)

                                                            cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGTCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAtt                                                                           >  2:72041/1‑121 (MQ=255)

                                                            cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTctcctc                                                                                                >  2:333204/1‑100 (MQ=255)

                                                            cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTctcctc                                                                                                <  1:333204/100‑1 (MQ=255)

                                                            cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaaaa                                                         >  2:518626/1‑139 (MQ=255)

                                                                      gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAc                                               <  1:797473/139‑1 (MQ=255)

                                                                      gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAc                                               <  1:895565/139‑1 (MQ=255)

                                                                      gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAc                                               <  1:62200/139‑1 (MQ=255)

                                                                      gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAc                                               <  1:518626/139‑1 (MQ=255)

                                                                              cTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTa                                       <  1:155743/139‑1 (MQ=255)

                                                                                   gAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                  <  1:712778/139‑1 (MQ=255)

                                                                                   gAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                  <  2:411654/139‑1 (MQ=255)

                                                                                                      tcCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGa               >  2:655987/1‑139 (MQ=255)

                                                                                                       ccGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGaa              >  2:146849/1‑139 (MQ=255)

                                                                                                         ggCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                  <  1:911188/117‑1 (MQ=255)

                                                                                                         ggCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                  >  2:911188/1‑117 (MQ=255)

                                                                                                                  gCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa   <  1:146849/139‑1 (MQ=255)

                                                                                                                  gCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa   <  1:107965/139‑1 (MQ=255)

                                                                                                                   cccGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGaa  <  1:655987/139‑1 (MQ=255)

GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAA  >  NC_000913/2263346‑2263599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A