Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,263,478	T→C	V6V (GTA→GTG)	fruB ←	fused fructose‑specific PTS enzymes: IIA component/HPr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,263,478	0	T	C	100.0%	244.1 / NA	67	V6V (GTA→GTG)	fruB	fused fructose‑specific PTS enzymes: IIA component/HPr component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/37); total (30/37)

TTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATAC  >  NC_000913/2263348‑2263609

tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATaa                                                                                                                             <  1:501569/139‑1 (MQ=255)

  tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACt                                                                                                                           >  2:198314/1‑139 (MQ=255)

        tCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACa                                                                                                                     <  1:1248389/139‑1 (MQ=255)

        tCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACa                                                                                                                     <  1:1313994/139‑1 (MQ=255)

           cgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAg                                                                                                                  <  2:99004/139‑1 (MQ=255)

                cAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTtctc                                                                                                             <  1:115173/139‑1 (MQ=255)

                        cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCAACCTGGCGAATCGCCGCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGACCATAGTTCTCCTCTCTTg                                                                                                     <  1:998823/139‑1 (MQ=255)

                          ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                                                                                                   >  1:391137/1‑139 (MQ=255)

                                  gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                           >  2:631972/1‑139 (MQ=255)

                                  gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                           >  2:823104/1‑139 (MQ=255)

                                  gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                           >  1:899846/1‑139 (MQ=255)

                                             cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGcc                                                                                >  1:847688/1‑139 (MQ=255)

                                                  ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACa                                                                                                                     <  1:846831/97‑1 (MQ=255)

                                                  ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACa                                                                                                                     >  2:846831/1‑97 (MQ=255)

                                                  ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTtc                                                                                                               >  2:19974/1‑103 (MQ=255)

                                                  ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTtc                                                                                                               <  1:19974/103‑1 (MQ=255)

                                                    ggCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATga                                                                         >  1:97629/1‑139 (MQ=255)

                                                       cTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGAt                                                                                                                               <  2:1633164/82‑1 (MQ=255)

                                                       cTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGAt                                                                                                                               >  1:1633164/1‑82 (MQ=255)

                                                       cTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaa                                                                      >  2:176950/1‑139 (MQ=255)

                                                        tGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGAt                                                                                                                               <  1:1090919/81‑1 (MQ=255)

                                                        tGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGAt                                                                                                                               >  2:1090919/1‑81 (MQ=255)

                                                          cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCctctc                                                                                                        >  1:605614/1‑102 (MQ=255)

                                                          cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCctctc                                                                                                        <  2:605614/102‑1 (MQ=255)

                                                          cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaaaa                                                                   >  2:268235/1‑139 (MQ=255)

                                                          cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAGTCAGCCTCTATGAGaaaaa                                                                   >  1:818826/1‑139 (MQ=255)

                                                               gcgcCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAgcg                                                              <  1:198314/139‑1 (MQ=255)

                                                               gcgcCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAgcg                                                              >  1:1673843/1‑139 (MQ=255)

                                                               gcgcCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCAGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAgcg                                                              <  2:818826/139‑1 (MQ=255)

                                                                cgccgcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAgcgc                                                             >  1:1390992/1‑139 (MQ=255)

                                                                    gcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAc                                                         >  2:1015424/1‑139 (MQ=255)

                                                                     cAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAtt                                                                                     >  1:212551/1‑110 (MQ=255)

                                                                     cAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAtt                                                                                     <  2:212551/110‑1 (MQ=255)

                                                                     cAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAAcc                                                        <  1:40257/139‑1 (MQ=255)

                                                                          aCCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCAAACCTGGCt                                                   <  2:647161/139‑1 (MQ=255)

                                                                                 gAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                            <  2:319770/139‑1 (MQ=255)

                                                                                 gAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                            <  1:268235/139‑1 (MQ=255)

                                                                                           ttcttTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGa                                  >  1:744810/1‑139 (MQ=255)

                                                                                            tcttTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGAt                                 <  2:1180334/139‑1 (MQ=255)

                                                                                            tcttTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGAt                                 <  1:631972/139‑1 (MQ=255)

                                                                                                    tcCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAg                                                                                                                  >  1:1440235/1‑50 (MQ=255)

                                                                                                    tcCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAg                                                                                                                  <  2:1440235/50‑1 (MQ=255)

                                                                                                     ccGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGaa                        >  1:414647/1‑139 (MQ=255)

                                                                                                      cGGCCTTTTCGCCTGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGaaa                       >  2:676195/1‑139 (MQ=255)

                                                                                                        gCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGc                                                                                 >  2:749239/1‑79 (MQ=255)

                                                                                                        gCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGc                                                                                 <  1:749239/79‑1 (MQ=255)

                                                                                                        gCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                            >  1:1233046/1‑116 (MQ=255)

                                                                                                        gCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                            <  2:1233046/116‑1 (MQ=255)

                                                                                                            tttCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                            <  1:361946/112‑1 (MQ=255)

                                                                                                            tttCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                            <  1:526980/112‑1 (MQ=255)

                                                                                                            tttCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                            >  2:361946/1‑112 (MQ=255)

                                                                                                            tttCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                            >  2:526980/1‑112 (MQ=255)

                                                                                                                gCCCGGATGGATGTCCTGCACGGGGAACTGGAACAGAGTTCTCGTCTCTTGCTGGAGTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGGCAACCGGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGGTTCAAGa             <  1:176950/139‑1 (MQ=255)

                                                                                                                   tggatggatGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAg          <  1:676195/138‑1 (MQ=255)

                                                                                                                   cGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAg          <  2:391137/139‑1 (MQ=255)

                                                                                                                   cGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAg          <  2:847688/139‑1 (MQ=255)

                                                                                                                   cGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAg          <  2:414647/139‑1 (MQ=255)

                                                                                                                   cGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAg          <  2:136864/139‑1 (MQ=255)

                                                                                                                   cGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAg          <  2:1673843/139‑1 (MQ=255)

                                                                                                                   cGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAg          <  2:1390992/139‑1 (MQ=255)

                                                                                                                     gatggatgTCCTGCACGGATAACTGGAACATAGTtctc                                                                                                             >  1:1760025/1‑38 (MQ=255)

                                                                                                                     gatggatgTCCTGCACGGATAACTGGAACATAGTtctc                                                                                                             <  2:1216632/38‑1 (MQ=255)

                                                                                                                     gatggatgTCCTGCACGGATAACTGGAACATAGTtctc                                                                                                             <  2:1760025/38‑1 (MQ=255)

                                                                                                                     gatggatgTCCTGCACGGATAACTGGAACATAGTtctc                                                                                                             >  1:1216632/1‑38 (MQ=255)

                                                                                                                     gatggatgTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTg                                                                                                  >  1:269241/1‑49 (MQ=255)

                                                                                                                     gatggatgTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTg                                                                                                  <  2:269241/49‑1 (MQ=255)

                                                                                                                           tgTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATAc  <  1:1015424/139‑1 (MQ=255)

TTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATAC  >  NC_000913/2263348‑2263609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A