Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,618,075	C→T	234 (TAG→TAA)	hda ←	ATPase regulatory factor involved in DnaA inactivation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,618,075	0	C	T	100.0%	143.9 / NA	47	234 (TAG→TAA)	hda	ATPase regulatory factor involved in DnaA inactivation
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/22); total (25/22)

GCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCC  >  NC_000913/2617943‑2618208

gCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAAc                                                                                                                                 <  2:845844/139‑1 (MQ=255)

         cTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAAtt                                                                                                                        >  2:858853/1‑139 (MQ=255)

                 aGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTAc                                                                                                                                    <  2:376300/119‑1 (MQ=255)

                 aGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTAc                                                                                                                                    >  1:376300/1‑119 (MQ=255)

                                              gTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCgg                                                                                   >  1:179033/1‑139 (MQ=255)

                                                       ggCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                          <  2:179033/139‑1 (MQ=255)

                                                         ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGaa                                                                                                                          >  2:688151/1‑89 (MQ=255)

                                                         ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGaa                                                                                                                          <  1:688151/89‑1 (MQ=255)

                                                         ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                        >  1:706281/1‑139 (MQ=255)

                                                             cGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGAt                                                                    >  2:230107/1‑139 (MQ=255)

                                                                     aTGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGa                                                            >  2:696864/1‑139 (MQ=255)

                                                                      tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGATTTTCTTTCACAAACGGAATGGTCAGCt                                                                                               <  2:555187/103‑1 (MQ=255)

                                                                      tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGAt                                                           >  1:717381/1‑139 (MQ=255)

                                                                      tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGCAATGGTCAGCt                                                                                               >  1:555187/1‑103 (MQ=255)

                                                                      tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCACGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATGCAACTGAt                                                           >  2:619765/1‑139 (MQ=255)

                                                                         aTTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                        <  2:706281/139‑1 (MQ=255)

                                                                         aTTAACTTCGCGTAGTTCGGATAAGGCGTTCACGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                        <  1:619765/139‑1 (MQ=255)

                                                                           tAACTTCGCGTAGTTCGGATAAGGCGTTTGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                                <  1:255035/129‑1 (MQ=255)

                                                                           tAACTTCGCGTAGTTCGGATAAGGCGTTTGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                                >  2:255035/1‑129 (MQ=255)

                                                                           tAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                      <  1:863437/139‑1 (MQ=255)

                                                                            aaCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                      <  2:644242/138‑1 (MQ=255)

                                                                            aaCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                      >  1:644242/1‑138 (MQ=255)

                                                                             aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAAt                                                                                                       <  2:351690/88‑1 (MQ=255)

                                                                             aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAAt                                                                                                       >  1:351690/1‑88 (MQ=255)

                                                                              cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTgcg                                                                                      >  2:457913/1‑104 (MQ=255)

                                                                              cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTgcg                                                                                      <  1:457913/104‑1 (MQ=255)

                                                                                  gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                        >  1:347686/1‑130 (MQ=255)

                                                                                  gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                        <  2:347686/130‑1 (MQ=255)

                                                                                  gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                        >  1:294037/1‑130 (MQ=255)

                                                                                  gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                        <  2:294037/130‑1 (MQ=255)

                                                                                   cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaaa                                              >  2:200797/1‑139 (MQ=255)

                                                                                   cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaaa                                              <  1:230107/139‑1 (MQ=255)

                                                                                                  gCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                      <  1:816316/116‑1 (MQ=255)

                                                                                                  gCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATAGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                      >  2:816316/1‑116 (MQ=255)

                                                                                                       cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                                >  2:396415/1‑101 (MQ=255)

                                                                                                       cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                                <  1:396415/101‑1 (MQ=255)

                                                                                                       cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCTAACGTCATAAATAGCGTGCGCATTTCTCTGt                          >  1:745451/1‑139 (MQ=255)

                                                                                                             gcATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGcc                    <  1:825662/139‑1 (MQ=255)

                                                                                                                 ccGACAATAAACACCTTATTTACAACTTCAGAATTTCATTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCtt                <  1:200797/139‑1 (MQ=255)

                                                                                                                     cgaTAAACACCTTATTTACAACTTCAGAATTTCTTTCCCAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACGGATCCAACGTCATAAATAGCTTGCGCATTTCTCTGTCGAGCCGCTTCAGc            <  2:717381/137‑1 (MQ=255)

                                                                                                                           acacCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa      >  1:749036/1‑139 (MQ=255)

                                                                                                                           acacCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa      >  2:473433/1‑139 (MQ=255)

                                                                                                                               cTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTATTCGACGTACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAAc     >  1:388526/1‑136 (MQ=255)

                                                                                                                               cTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTATTCGACGTACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAAc     <  2:388526/136‑1 (MQ=255)

                                                                                                                               cTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGcc  >  2:450483/1‑139 (MQ=255)

                                                                                                                                ttATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGc                                                                         >  1:55743/1‑67 (MQ=255)

                                                                                                                                ttATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGc                                                                         <  2:55743/67‑1 (MQ=255)

GCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCC  >  NC_000913/2617943‑2618208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A