Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,937,802	A→G	D112G (GAC→GGC)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,937,802	0	A	G	100.0%	170.7 / NA	47	D112G (GAC→GGC)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/26); total (21/26)

AACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCT  >  NC_000913/2937670‑2937936

agctgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATGAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAatat                                                                                                                                  >  2:558131/3‑139 (MQ=255)

  ctgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTg                                                                                                                                <  1:148532/139‑1 (MQ=255)

  ctgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTg                                                                                                                                <  1:723814/139‑1 (MQ=255)

  ctgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTg                                                                                                                                >  2:534740/1‑139 (MQ=255)

          aTGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTa                                                                                                                        >  1:903692/1‑139 (MQ=255)

           tGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTaa                                                                                                                       >  1:120083/1‑139 (MQ=255)

                   ccGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGca                                                                                                               <  2:919218/139‑1 (MQ=255)

                   ccGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGca                                                                                                               >  1:319070/1‑139 (MQ=255)

                                         tttGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTg                                                                                         <  1:534740/139‑1 (MQ=255)

                                             gtgtGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGaa                                                                                                                              >  2:374705/1‑98 (MQ=255)

                                             gtgtGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGaa                                                                                                                              <  1:374705/98‑1 (MQ=255)

                                             gtgtGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGt                                                                                     >  2:316933/1‑139 (MQ=255)

                                                   aTGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGc                                                                               >  2:256849/1‑139 (MQ=255)

                                                     ggCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCt                                                                             >  1:737050/1‑139 (MQ=255)

                                                       cgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCttt                                                                           >  1:427749/1‑139 (MQ=255)

                                                       cgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCttt                                                                           >  1:383209/1‑139 (MQ=255)

                                                       cgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCttt                                                                           <  2:427749/139‑1 (MQ=255)

                                                       cgcTCTGGTAGATAAGCAAGGCAATCTGATCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCttt                                                                           <  1:558131/139‑1 (MQ=255)

                                                        gcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAg                                                                                                   <  1:553728/114‑1 (MQ=255)

                                                        gcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAg                                                                                                   >  2:553728/1‑114 (MQ=255)

                                                              gTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                    <  1:316933/139‑1 (MQ=255)

                                                              gTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                    <  2:401806/139‑1 (MQ=255)

                                                               tAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCaa                                                                   <  2:737050/139‑1 (MQ=255)

                                                               tAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCaa                                                                   <  1:14141/139‑1 (MQ=255)

                                                                 gATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 <  2:675418/139‑1 (MQ=255)

                                                                 gATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 <  2:903692/139‑1 (MQ=255)

                                                                 gATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 <  2:371625/139‑1 (MQ=255)

                                                                 gATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 <  2:94235/139‑1 (MQ=255)

                                                                  aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGGCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTAAATAc                                                                <  2:131195/139‑1 (MQ=255)

                                                                   tAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                               <  2:383209/139‑1 (MQ=255)

                                                                      gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 <  1:471409/134‑1 (MQ=255)

                                                                      gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 >  2:471409/1‑134 (MQ=255)

                                                                         aGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                               >  2:227348/1‑133 (MQ=255)

                                                                         aGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                               <  1:227348/133‑1 (MQ=255)

                                                                                cTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                               >  2:480676/1‑126 (MQ=255)

                                                                                cTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                               <  1:480676/126‑1 (MQ=255)

                                                                                        tCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 >  2:523272/1‑116 (MQ=255)

                                                                                        tCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 <  1:523272/116‑1 (MQ=255)

                                                                                             ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCaa                                                                   >  1:718459/1‑109 (MQ=255)

                                                                                             ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCaa                                                                   <  2:718459/109‑1 (MQ=255)

                                                                                                  aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 >  1:65312/1‑106 (MQ=255)

                                                                                                  aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                 <  2:65312/106‑1 (MQ=255)

                                                                                                       gAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCca                           >  2:112870/1‑139 (MQ=255)

                                                                                                        aaaTGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                          <  1:256849/139‑1 (MQ=255)

                                                                                                                                aTGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                    >  1:359238/1‑73 (MQ=255)

                                                                                                                                aTGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                    <  2:359238/73‑1 (MQ=255)

                                                                                                                                aTGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCt  >  2:148168/1‑139 (MQ=255)

AACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCT  >  NC_000913/2937670‑2937936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A