Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A4 F1 I1 R1 | 765 | 39.6 | 1867486 | 94.4% | 1762906 | 108.0 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,994,415 | G→A | pseudogene (34/60 nt) | pbl → | pseudogene, peptidoglycan‑binding enzyme family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,994,415 | 0 | G | A | 100.0% | 227.6 / NA | 71 | pseudogene (34/60 nt) | pbl | pseudogene, peptidoglycan‑binding enzyme family |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (37/34); total (37/34) | |||||||||||
GTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTC > NC_000913/2994297‑2994545 | gtgGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCa > 1:579879/1‑139 (MQ=255) ccTGGTGACTTATAATGCGTGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 2:834073/139‑1 (MQ=255) ccTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 2:6106/139‑1 (MQ=255) ccTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTCAATGAACTAAACAATCGTAACGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 2:602217/139‑1 (MQ=255) aCTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑tttttttt < 2:579879/139‑1 (MQ=255) tataATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 2:5603/129‑1 (MQ=255) tataATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCATAAGGCTTCAGCAAAAGGAGCCTAAAGCctc‑t > 1:5603/1‑129 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTATTGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑tttttt < 1:627906/125‑1 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 2:215553/1‑120 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:215553/120‑1 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑tttttt > 2:627906/1‑125 (MQ=255) ttggaaaTGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAAATAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAacac < 1:758633/134‑1 (MQ=255) aaCCAAAAAAAGAGACAGCAATATGCCCGAAAATGTATATTGTATATACTAGGCTTAATGAGCTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAg > 2:602143/1‑139 (MQ=255) aaaaGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:385508/98‑1 (MQ=255) aaaaGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 2:385508/1‑98 (MQ=255) agagACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGAt < 2:552817/139‑1 (MQ=255) tatGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATa < 1:803977/139‑1 (MQ=255) tatatatTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACAt < 1:210599/139‑1 (MQ=255) tGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATa > 1:646147/1‑119 (MQ=255) tGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATa < 2:646147/119‑1 (MQ=255) gTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTACAGCAACATGAGACTATAGCCTG‑TTTTTATTCTATAGTGCCAACTCCCATAAGTATAGCCGTCTTAATCATCTCGATACCACTATGAACATCTTTTttc > 2:256637/1‑139 (MQ=255) tatataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 2:863644/63‑1 (MQ=255) tatataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 1:863644/1‑63 (MQ=255) tatataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAg < 1:283887/97‑1 (MQ=255) tatataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAg > 2:283887/1‑97 (MQ=255) tatataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttct < 2:110112/139‑1 (MQ=255) atataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttctt > 1:691465/1‑139 (MQ=255) tataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCaa > 1:160367/1‑108 (MQ=255) tataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCaa < 2:160367/108‑1 (MQ=255) tataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttcttc > 2:785217/1‑139 (MQ=255) ataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:671747/60‑1 (MQ=255) ataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 2:671747/1‑60 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:677375/58‑1 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 2:677375/1‑58 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATc < 2:520102/103‑1 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATc > 1:520102/1‑103 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATg > 2:7075/1‑139 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATg > 2:909614/1‑139 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 2:776637/56‑1 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 1:776637/1‑56 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCa > 2:295983/1‑73 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCa < 1:295983/73‑1 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACt > 2:254256/1‑105 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACt < 1:254256/105‑1 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGat > 2:878060/1‑139 (MQ=255) aGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGata < 2:296136/139‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 2:812450/1‑53 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 2:43806/1‑53 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:43806/53‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:812450/53‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑ttt > 2:357265/1‑55 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑ttt > 2:380290/1‑55 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑ttt < 1:380290/55‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑ttt < 1:357265/55‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa > 2:316166/1‑72 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa > 2:860174/1‑72 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa < 1:860174/72‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa < 1:316166/72‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAAc < 2:374475/118‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAAc > 1:374475/1‑118 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 2:93482/1‑139 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 1:786470/1‑139 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt < 1:7075/139‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 1:448131/1‑139 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 1:225990/1‑139 (MQ=255) aaTGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 2:175739/1‑49 (MQ=255) aaTGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:175739/49‑1 (MQ=255) aaTGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGt > 1:312306/1‑139 (MQ=255) aTGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTc > 1:72120/1‑139 (MQ=255) cTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttct > 1:909478/1‑119 (MQ=255) cTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttct < 2:909478/119‑1 (MQ=255) | GTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTC > NC_000913/2994297‑2994545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |