Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A13 F1 I2 R1 | 762 | 35.5 | 1545288 | 95.5% | 1475750 | 117.0 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,994,415 | G→A | pseudogene (34/60 nt) | pbl → | pseudogene, peptidoglycan‑binding enzyme family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,994,415 | 0 | G | A | 100.0% | 248.2 / NA | 77 | pseudogene (34/60 nt) | pbl | pseudogene, peptidoglycan‑binding enzyme family |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (45/32); total (45/32) | |||||||||||
TTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGA > NC_000913/2994287‑2994550 | ttttCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTaa > 2:484970/1‑139 (MQ=255) gTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCa > 1:563145/1‑139 (MQ=255) ttGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGCGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAg < 2:486452/139‑1 (MQ=255) aGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAAt < 2:563145/139‑1 (MQ=255) aGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAAt < 1:484970/139‑1 (MQ=255) cGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGcc > 2:384576/1‑139 (MQ=255) ccTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:384576/139‑1 (MQ=255) ccTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 2:528819/139‑1 (MQ=255) ccTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 1:528819/1‑139 (MQ=255) ccTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:722049/139‑1 (MQ=255) tataATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTtata < 1:447455/139‑1 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa < 1:672062/139‑1 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa < 2:381655/139‑1 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa < 2:212606/139‑1 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa < 2:116229/139‑1 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa > 2:124690/1‑139 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa > 1:116229/1‑139 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa > 1:381655/1‑139 (MQ=255) ggTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa > 2:672062/1‑139 (MQ=255) gTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTTATATAGTGCaaa < 1:132879/139‑1 (MQ=255) ttttGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAaca > 2:41861/1‑139 (MQ=255) aaCCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAg > 2:259786/1‑139 (MQ=255) agagACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGAt < 1:124690/139‑1 (MQ=255) gagaCAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATc < 1:614526/139‑1 (MQ=255) agaCAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCa > 2:572859/1‑139 (MQ=255) cAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACt < 1:572859/139‑1 (MQ=255) cAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACAccc < 1:250200/101‑1 (MQ=255) cAAAATATATATTGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACAccc > 2:250200/1‑101 (MQ=255) tGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 1:215692/1‑65 (MQ=255) tGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 2:215692/65‑1 (MQ=255) tGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCct < 1:168052/63‑1 (MQ=255) tGTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCct > 2:168052/1‑63 (MQ=255) gTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATa < 1:661898/118‑1 (MQ=255) gTATATACCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATa > 2:661898/1‑118 (MQ=255) atataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTtat < 1:132919/71‑1 (MQ=255) atataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTtat > 2:132919/1‑71 (MQ=255) atataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCaa > 1:738584/1‑109 (MQ=255) atataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCaa < 2:738584/109‑1 (MQ=255) ataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 2:188676/60‑1 (MQ=255) ataCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t > 1:188676/1‑60 (MQ=255) acgaGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCctc‑t < 1:245192/55‑1 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATg > 1:94774/1‑139 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATg > 1:399332/1‑139 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATg > 1:140539/1‑139 (MQ=255) aCCAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGActc‑t > 2:245192/1‑58 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTTTATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGat > 2:502217/1‑139 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaa > 1:760182/1‑74 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaa < 2:760182/74‑1 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGat > 1:250481/1‑139 (MQ=255) cAGGCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGat > 2:668245/1‑139 (MQ=255) tcTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa < 1:640231/71‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATg < 2:150761/39‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATg > 1:150761/1‑39 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGc > 1:193234/1‑49 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGc < 2:193234/49‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGc > 1:77952/1‑69 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGc < 2:77952/69‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCaaa > 2:640231/1‑72 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACt < 2:7088/102‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACt < 2:346058/102‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACt > 1:7088/1‑102 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACt > 1:346058/1‑102 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAAt < 2:86055/125‑1 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAAt > 1:86055/1‑125 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 2:607151/1‑139 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 2:450735/1‑139 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 1:661376/1‑139 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 2:674310/1‑139 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 1:70514/1‑139 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 1:114378/1‑139 (MQ=255) gCTTAATGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTGCATGATAtt > 2:522539/1‑139 (MQ=255) aaTGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGt > 2:499716/1‑139 (MQ=255) aTGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGAt > 1:377190/1‑92 (MQ=255) aTGAACTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGAt < 2:377190/92‑1 (MQ=255) cTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATc > 1:530925/1‑88 (MQ=255) cTAAACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGa > 1:68738/1‑139 (MQ=255) cTAAACAATCGTAAGGCTACAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATAtt > 2:428172/1‑129 (MQ=255) | TTTTCACGTTGTGGAGTAACGTGGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTC‑TTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGA > NC_000913/2994287‑2994550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |