Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,424,051	G→A	D118N (GAC→AAC)	ulaF →	L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,424,051	0	G	A	100.0%	131.4 / NA	41	D118N (GAC→AAC)	ulaF	L‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/17); total (24/17)

CTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAA  >  NC_000913/4423923‑4424183

cTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTActtctt                                                                                                                            <  2:440343/139‑1 (MQ=255)

                     aCCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCATGCCAACTACTTCTTTGGCGACATTCCGTGTAcgcg                                                                                                       >  2:465330/1‑139 (MQ=255)

                           gtTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGtt                                                                                                 <  2:814839/139‑1 (MQ=255)

                            ttACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTa                                                                                                >  2:120711/1‑139 (MQ=255)

                                   tCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaag                                                                                         >  1:319092/1‑139 (MQ=255)

                                     gCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCgaagaa                                                                                       >  1:249647/1‑139 (MQ=255)

                                              cATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAg                                                                              >  2:343275/1‑139 (MQ=255)

                                                   tCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGaagaag                                                                                      >  2:768367/1‑126 (MQ=255)

                                                   tCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGaagaag                                                                                      <  1:768367/126‑1 (MQ=255)

                                                     cATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                       <  1:343275/139‑1 (MQ=255)

                                                           cactccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaac                                                                 <  2:711696/139‑1 (MQ=255)

                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCg                                                                                                                       >  1:37306/1‑83 (MQ=255)

                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCg                                                                                                                       <  2:37306/83‑1 (MQ=255)

                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                       >  2:841576/1‑131 (MQ=255)

                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGt                                                                       <  1:841576/131‑1 (MQ=255)

                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  2:353967/1‑139 (MQ=255)

                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  2:314085/1‑139 (MQ=255)

                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  1:870794/1‑139 (MQ=255)

                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  1:512408/1‑139 (MQ=255)

                                                             ctccactcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactg                                                               >  1:140060/1‑139 (MQ=255)

                                                                  ctcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAacac                                                          >  1:997/1‑139 (MQ=255)

                                                                  ctcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAacac                                                          >  1:383570/1‑139 (MQ=255)

                                                                  ctcATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGGGAGGGGCAGGTTTGAACTGAacac                                                          >  1:462826/1‑139 (MQ=255)

                                                                    cATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCg                                                        >  1:215805/1‑139 (MQ=255)

                                                                       gCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCa                                                     >  1:835583/1‑139 (MQ=255)

                                                                       gCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCa                                                     >  2:616353/1‑139 (MQ=255)

                                                                            cGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTg                                                <  2:383570/139‑1 (MQ=255)

                                                                              cATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGAt                                              <  2:215805/139‑1 (MQ=255)

                                                                              cATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTGAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGAt                                              <  1:383324/139‑1 (MQ=255)

                                                                                 gggCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTAt                                           >  2:404834/1‑139 (MQ=255)

                                                                                        ggCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACg                                    <  2:835583/139‑1 (MQ=255)

                                                                                         gCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                            <  1:772001/98‑1 (MQ=255)

                                                                                         gCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAggg                                                                            >  2:772001/1‑98 (MQ=255)

                                                                                             ggCTGGGGAACCCGGTGTCAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTTCGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGACCTGAACACCGGCAAAGTGATTATCGAAACGCTggg                               <  2:318320/139‑1 (MQ=255)

                                                                                             ggCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGc                                                                                                                        <  2:911476/50‑1 (MQ=255)

                                                                                             ggCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGc                                                                                                                        >  1:911476/1‑50 (MQ=255)

                                                                                             ggCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAagag                                                                                    >  2:913490/1‑86 (MQ=255)

                                                                                             ggCTGGCGATCCCGGCGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAagag                                                                                    <  1:913490/86‑1 (MQ=255)

                                                                                                       cccGGTGTTAGGCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAg                     >  2:312895/1‑139 (MQ=255)

                                                                                                                  gCACCACGCACGCCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATAc          <  1:353967/139‑1 (MQ=255)

                                                                                                                          cacgcCAACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGaa  <  1:404834/139‑1 (MQ=255)

CTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAA  >  NC_000913/4423923‑4424183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A