Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,143	A→G	E576E (GAA→GAG)	ybbP →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,143	0	A	G	100.0%	170.5 / NA	48	E576E (GAA→GAG)	ybbP	putative ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/24); total (24/24)

GCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTT  >  NC_000913/522014‑522271

gCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTg                                                                                                                         >  1:702586/1‑139 (MQ=255)

   gACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCg                                                                                                                      <  2:1272588/139‑1 (MQ=255)

     cAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGaa                                                                                                                    <  2:669993/139‑1 (MQ=255)

                  aGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGtt                                                                                                       >  2:1084540/1‑139 (MQ=255)

                   gATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATggagga                                                                                                                                >  2:592134/1‑113 (MQ=255)

                   gATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATggagga                                                                                                                                >  2:1127882/1‑113 (MQ=255)

                   gATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGtcg                                                                                                      <  2:702586/139‑3 (MQ=255)

                     tGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATCATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  1:902440/139‑1 (MQ=255)

                     tGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  1:1427419/139‑1 (MQ=255)

                      gAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGccc                                                                                                   >  2:1454897/1‑139 (MQ=255)

                      gAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGccc                                                                                                   >  2:1341987/1‑139 (MQ=255)

                                    cgAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCg                                                                                                                      >  1:1140012/1‑106 (MQ=255)

                                    cgAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCg                                                                                                                      <  2:1140012/106‑1 (MQ=255)

                                    cgAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTg                                                                                     <  1:1454897/139‑1 (MQ=255)

                                      aaCTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    >  1:130239/1‑122 (MQ=255)

                                      aaCTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  2:130239/122‑1 (MQ=255)

                                           aaTCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTa                                                                              >  1:915226/1‑139 (MQ=255)

                                                    tGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATa                                                                     <  1:1341987/139‑1 (MQ=255)

                                                     ggCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    >  2:163677/1‑107 (MQ=255)

                                                     ggCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  1:163677/107‑1 (MQ=255)

                                                       cAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATAccc                                                                  >  2:1522331/1‑139 (MQ=255)

                                                           aTACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGGGAAACGCTTAAACGt                                                                                                        <  2:1129674/97‑1 (MQ=255)

                                                           aTACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGt                                                                                                        >  1:1129674/1‑97 (MQ=255)

                                                                             aTCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGaa                                                                                                                    >  2:1183455/1‑67 (MQ=255)

                                                                             aTCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGaa                                                                                                                    <  1:1183455/67‑1 (MQ=255)

                                                                                 gATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGc                                        >  2:1304960/1‑139 (MQ=255)

                                                                                  aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACg                                                                                                         >  1:859596/1‑73 (MQ=255)

                                                                                  aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACg                                                                                                         <  2:859596/73‑1 (MQ=255)

                                                                                  aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCt                                                                                                  <  2:1269325/80‑1 (MQ=255)

                                                                                  aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCt                                                                                                  >  1:1269325/1‑80 (MQ=255)

                                                                                  aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGcc                                       >  2:608673/1‑139 (MQ=255)

                                                                                   ttGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTa                                                                              >  2:209285/1‑99 (MQ=255)

                                                                                   ttGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTa                                                                              <  1:209285/99‑1 (MQ=255)

                                                                                   ttGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                                                  >  2:711624/1‑127 (MQ=255)

                                                                                   ttGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                                                  <  1:711624/127‑1 (MQ=255)

                                                                                      tCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTgcg                                   >  2:890664/1‑139 (MQ=255)

                                                                                       cGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTgcgc                                  >  2:70132/1‑139 (MQ=255)

                                                                                         ccGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  1:1428342/71‑1 (MQ=255)

                                                                                         ccGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    >  2:1428342/1‑71 (MQ=255)

                                                                                          cGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    <  2:1310054/70‑1 (MQ=255)

                                                                                          cGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                    >  1:1310054/1‑70 (MQ=255)

                                                                                          cGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCaaa                               <  2:915226/139‑1 (MQ=255)

                                                                                               aCTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGa                          <  1:154321/139‑1 (MQ=255)

                                                                                                    ccgccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTggg                     >  2:1444324/1‑139 (MQ=255)

                                                                                                    ccgccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTggg                     <  1:1522331/139‑1 (MQ=255)

                                                                                                       ccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGaaa                  >  2:1402386/1‑139 (MQ=255)

                                                                                                                gATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGc         <  1:608673/139‑1 (MQ=255)

                                                                                                                  tGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCt       >  2:1388693/1‑139 (MQ=255)

                                                                                                                     aGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAAt    <  1:116821/139‑1 (MQ=255)

                                                                                                                       tgtCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAAttt  <  1:890664/139‑1 (MQ=255)

GCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTT  >  NC_000913/522014‑522271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A