Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,143	A→G	E576E (GAA→GAG)	ybbP →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,143	0	A	G	100.0%	117.8 / NA	33	E576E (GAA→GAG)	ybbP	putative ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/17); total (16/17)

CAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTC  >  NC_000913/522019‑522272

cAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGaa                                                                                                                     <  1:620929/139‑1 (MQ=255)

          tGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAAc                                                                                                           >  2:547/1‑139 (MQ=255)

            aaGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGt                                                                                                         <  1:566123/139‑1 (MQ=255)

                tGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                     >  1:581078/1‑139 (MQ=255)

                      gcTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGt                                                                                               >  1:212346/1‑139 (MQ=255)

                      gcTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGt                                                                                               >  2:413392/1‑139 (MQ=255)

                       cTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTg                                                                                              <  2:233789/139‑1 (MQ=255)

                       cTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTg                                                                                              >  1:502908/1‑139 (MQ=255)

                        ttAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGa                                                                                             >  1:486203/1‑139 (MQ=255)

                               cgAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTACCCTCGGTGATACCGTg                                                                                      <  2:502908/139‑1 (MQ=255)

                                            aCCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCg                                                                         <  2:581078/139‑1 (MQ=255)

                                            aCCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCg                                                                         <  2:659559/139‑1 (MQ=255)

                                             ccTGGCAAAATACGCGACCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTG‑CCTCGGTGATACCGTGACTTTTATGGGCGAt                                                                       >  1:578714/1‑139 (MQ=255)

                                                         cgcgGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGtt                                                            <  1:547/139‑1 (MQ=255)

                                                         cgcgGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGtt                                                            <  2:212346/139‑1 (MQ=255)

                                                            gacccGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTG‑CCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgc                                                        <  2:578714/137‑1 (MQ=255)

                                                                  aTCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                                                   >  2:711828/1‑139 (MQ=255)

                                                                      tAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                     <  1:325096/85‑1 (MQ=255)

                                                                      tAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                     >  2:325096/1‑85 (MQ=255)

                                                                       aaTCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGa                                              <  1:253969/139‑1 (MQ=255)

                                                                         tCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGAcc                                            <  1:320021/139‑1 (MQ=255)

                                                                             aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgcg                                                       <  1:127403/124‑1 (MQ=255)

                                                                             aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTCACTTTTATGGGCGATACCCAGGAGTTCcgcg                                                       >  2:127403/1‑124 (MQ=255)

                                                                                   gCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTg                                                                                      >  1:24433/1‑87 (MQ=255)

                                                                                   gCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTg                                                                                      <  2:24433/87‑1 (MQ=255)

                                                                                             ggCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTg                        >  1:629733/1‑139 (MQ=255)

                                                                                                cgccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGa                     <  1:62698/139‑1 (MQ=255)

                                                                                                  ccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGAc                                             <  1:72254/113‑1 (MQ=255)

                                                                                                  ccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGAc                                             >  2:72254/1‑113 (MQ=255)

                                                                                                      aaGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCt               >  2:714735/1‑139 (MQ=255)

                                                                                                        gCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACg                                                                                                          >  2:180575/1‑46 (MQ=255)

                                                                                                        gCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACg                                                                                                          <  1:180575/46‑1 (MQ=255)

                                                                                                                   gtCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTc  >  2:249586/1‑139 (MQ=255)

CAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTC  >  NC_000913/522019‑522272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A