Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,459,052	G→A	263 (TAG→TAA)	paaG →	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,459,052	0	G	A	100.0%	152.4 / NA	47	263 (TAG→TAA)	paaG	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/23); total (24/23)

TGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAA  >  NC_000913/1458920‑1459186

tGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACatgat                                                                                                                                  >  1:1396444/1‑139 (MQ=255)

            gCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                      <  2:1535327/139‑1 (MQ=255)

                 aTACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                      <  2:683571/134‑1 (MQ=255)

                 aTACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                      >  1:683571/1‑134 (MQ=255)

                  tACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACtgt                                                                                                                <  2:1396444/139‑1 (MQ=255)

                  tACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACtgt                                                                                                                <  1:1605970/139‑1 (MQ=255)

                                 ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                                 <  2:1290917/139‑1 (MQ=255)

                                 ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                                 >  1:976150/1‑139 (MQ=255)

                                   aaCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCg                                                                                               <  2:490001/139‑1 (MQ=255)

                                       tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACACTCACCGCAGTTCACGGGGAAATAACATGATGATa                                                                                                                              >  2:279512/1‑104 (MQ=255)

                                       tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACACTCACCGCAGTTCACGGGGAAATAACATGATGATa                                                                                                                              <  1:279512/104‑1 (MQ=255)

                                         aCTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCaaa                                                                                                                    >  1:657990/1‑112 (MQ=255)

                                         aCTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCaaa                                                                                                                    <  2:657990/112‑1 (MQ=255)

                                            aTCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                      >  1:737917/1‑107 (MQ=255)

                                            aTCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                      <  2:737917/107‑1 (MQ=255)

                                                gCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCa                                                                                         <  1:178340/132‑1 (MQ=255)

                                                gCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCCCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCa                                                                                         >  2:178340/1‑132 (MQ=255)

                                                    cTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCAt                                                                                        <  1:708753/129‑1 (MQ=255)

                                                    cTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCAt                                                                                        >  2:708753/1‑129 (MQ=255)

                                                       gCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCa                                                                                         >  2:645929/1‑125 (MQ=255)

                                                       gCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCa                                                                                         <  1:645929/125‑1 (MQ=255)

                                                                aGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGtt                                                                  >  2:103842/1‑139 (MQ=255)

                                                                     aGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                             <  2:976150/139‑1 (MQ=255)

                                                                          aTCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAtgtg                                                                                                                        <  2:348171/75‑1 (MQ=255)

                                                                          aTCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAtgtg                                                                                                                        >  1:348171/1‑75 (MQ=255)

                                                                          aTCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                      <  1:676438/77‑1 (MQ=255)

                                                                          aTCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                      >  2:676438/1‑77 (MQ=255)

                                                                          aTCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTc                                                        >  2:1041330/1‑139 (MQ=255)

                                                                                          tGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACGCCAGGtttt                                        >  1:88993/1‑139 (MQ=255)

                                                                                               tCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa                                                                                                            <  2:175199/66‑1 (MQ=255)

                                                                                               tCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa                                                                                                            >  1:175199/1‑66 (MQ=255)

                                                                                                    gCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGAc                              >  1:486636/1‑139 (MQ=255)

                                                                                                         aCGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                                 >  2:1551799/1‑67 (MQ=255)

                                                                                                         aCGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                                 <  1:1551799/67‑1 (MQ=255)

                                                                                                              cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                    >  2:440470/1‑139 (MQ=255)

                                                                                                                    aGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                             <  2:542818/92‑1 (MQ=255)

                                                                                                                    aGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                             >  1:542818/1‑92 (MQ=255)

                                                                                                                      ttCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCa                                                           <  2:341714/92‑1 (MQ=255)

                                                                                                                      ttCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCa                                                           >  1:341714/1‑92 (MQ=255)

                                                                                                                       tCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAc                                                                                           >  2:799247/1‑59 (MQ=255)

                                                                                                                       tCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAc                                                                                           <  1:799247/59‑1 (MQ=255)

                                                                                                                        cACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACGCCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGAc          <  2:88993/139‑1 (MQ=255)

                                                                                                                         aCGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa                                                                                                            <  2:639363/40‑1 (MQ=255)

                                                                                                                         aCGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa                                                                                                            >  1:639363/1‑40 (MQ=255)

                                                                                                                             ggAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCcgcg     <  1:1041330/139‑1 (MQ=255)

                                                                                                                                aaTAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGGTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCaa  >  1:1055836/1‑139 (MQ=255)

                                                                                                                                aaTAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCaa  >  2:1438951/1‑139 (MQ=255)

TGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAA  >  NC_000913/1458920‑1459186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A