Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
766 |
55.6 |
2631458 |
94.7% |
2491990 |
109.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,459,052 |
G→A |
*263* (TAG→TAA) |
paaG → |
1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,459,052 | 0 | G | A | 100.0%
| 145.6
/ NA
| 46 | *263* (TAG→TAA) | paaG | 1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/28); total (18/28) |
GCGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCG > NC_000913/1458916‑1459181
|
gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑a < 1:675424/139‑1 (MQ=255)
gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑a > 2:14842/1‑139 (MQ=255)
gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑a < 1:447131/139‑1 (MQ=255)
gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑a < 1:1253759/139‑1 (MQ=255)
cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑at < 2:855888/139‑1 (MQ=255)
tGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑atgat < 1:14842/139‑1 (MQ=255)
tGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑atgat < 1:115585/139‑1 (MQ=255)
aaaCCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑Atgatga > 2:347827/1‑139 (MQ=255)
aCCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa < 2:66882/139‑1 (MQ=255)
aTACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑atg > 2:524560/1‑120 (MQ=255)
aTACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑atg < 1:524560/120‑1 (MQ=255)
aTACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACtg > 2:609493/1‑139 (MQ=255)
aCTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGt > 1:976487/1‑139 (MQ=255)
gATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTg > 1:281511/1‑136 (MQ=255)
gATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACGGTGGCAGTg < 2:281511/136‑1 (MQ=255)
ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg < 1:57078/139‑1 (MQ=255)
aaCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCg < 1:1142050/139‑1 (MQ=255)
aCGTGACTATCTGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg < 1:852983/139‑1 (MQ=255)
aCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg < 1:609879/139‑1 (MQ=255)
tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAc > 1:790258/1‑139 (MQ=255)
gACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCAcc > 1:598082/1‑139 (MQ=255)
aCTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCa < 2:231197/139‑1 (MQ=255)
aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg > 2:588160/1‑139 (MQ=255)
aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg < 2:723028/139‑1 (MQ=255)
tGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCAt < 1:609493/139‑1 (MQ=255)
gCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa > 1:1251281/1‑88 (MQ=255)
gCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa < 2:1251281/88‑1 (MQ=255)
gacggaGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTg < 2:1060291/139‑1 (MQ=255)
aGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc < 2:598082/139‑1 (MQ=255)
gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTgg > 2:1204616/1‑76 (MQ=255)
gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTgg < 1:1204616/76‑1 (MQ=255)
gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACAcc < 1:898081/139‑1 (MQ=255)
tCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAgg < 2:790258/139‑1 (MQ=255)
tGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa < 1:477066/53‑1 (MQ=255)
tGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATa > 2:477066/1‑53 (MQ=255)
cGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCAt > 1:385850/1‑89 (MQ=255)
cGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCAt < 2:385850/89‑1 (MQ=255)
cGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTAc < 1:588160/139‑1 (MQ=255)
gTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGtttt < 1:506133/139‑1 (MQ=255)
ttCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcaggc < 2:184416/97‑1 (MQ=255)
ttCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcaggc > 1:184416/1‑97 (MQ=255)
ggCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACtgt < 1:1184571/58‑1 (MQ=255)
ggCTAAACGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACtgt > 2:1184571/1‑58 (MQ=255)
aCGCTCACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTc > 1:1137184/1‑139 (MQ=255)
cACCGCAGTTCACGGGGAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg > 1:775941/1‑139 (MQ=255)
ggggAAATAAC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCcg > 2:640948/1‑139 (MQ=255)
|
GCGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGC‑‑‑ATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCG > NC_000913/1458916‑1459181
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
