Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,016,095	G→A	L76L (CTG→CTA)	fliH →	negative regulator of FliI ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,016,095	0	G	A	100.0%	148.3 / NA	46	L76L (CTG→CTA)	fliH	negative regulator of FliI ATPase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/24); total (22/24)

CATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAG  >  NC_000913/2015963‑2016216

catTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAg                                                                                                                     >  1:608206/1‑139 (MQ=255)

catTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAg                                                                                                                     >  1:265636/1‑139 (MQ=255)

    gAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGc                                                                                                                 <  2:1687924/139‑1 (MQ=255)

          gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAg                                                                                                            >  1:144549/1‑138 (MQ=255)

          gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAg                                                                                                            <  2:144549/138‑1 (MQ=255)

          gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGc                                                                                                           <  2:315008/139‑1 (MQ=255)

          gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGc                                                                                                           >  1:211000/1‑139 (MQ=255)

                    gCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTgac                                                                                                 >  2:1189686/1‑137 (MQ=255)

                           gcagcaACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                          <  2:1047555/139‑1 (MQ=255)

                               caACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                          <  2:554007/135‑1 (MQ=255)

                               caACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                          >  1:554007/1‑135 (MQ=255)

                                      cgcAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTcaaca                                                                               <  2:32804/139‑1 (MQ=255)

                                      cgcAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGACAGAGGCGAAGTCTcaaca                                                                               <  1:1189686/139‑1 (MQ=255)

                                                      ggCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGcc                                                               <  1:758589/139‑1 (MQ=255)

                                                             gAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATgc                                                        <  2:265636/139‑1 (MQ=255)

                                                                   ggTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAgggg                                                                                                                  >  2:924115/1‑75 (MQ=255)

                                                                   ggTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAgggg                                                                                                                  <  1:924115/75‑1 (MQ=255)

                                                                   ggTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGGCCGGATGCAGCAAc                                                  >  2:719650/1‑139 (MQ=255)

                                                                       aTCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGt                                              <  2:211000/139‑1 (MQ=255)

                                                                        tCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGAt                                                          <  2:1111566/126‑1 (MQ=255)

                                                                        tCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGAt                                                          >  1:1111566/1‑126 (MQ=255)

                                                                             cGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAg                                                                                                            <  1:1537413/71‑1 (MQ=255)

                                                                             cGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAg                                                                                                            >  2:1537413/1‑71 (MQ=255)

                                                                               ggTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAAt                                      >  2:1480565/1‑139 (MQ=255)

                                                                                       cGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                          >  1:1484831/1‑79 (MQ=255)

                                                                                       cGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg                                                                                          <  2:1484831/79‑1 (MQ=255)

                                                                                         aaGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGt                                                                                                      <  1:1543436/65‑1 (MQ=255)

                                                                                         aaGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGt                                                                                                      >  2:1543436/1‑65 (MQ=255)

                                                                                          aGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGc                                                                                                                 <  2:994686/53‑1 (MQ=255)

                                                                                          aGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGc                                                                                                                 >  1:994686/1‑53 (MQ=255)

                                                                                           ggTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTAccc                          >  2:189460/1‑139 (MQ=255)

                                                                                              cGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                       <  2:92181/139‑1 (MQ=255)

                                                                                              cGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                       <  1:189460/139‑1 (MQ=255)

                                                                                                       ggTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTcaaca                                                                               >  2:741915/1‑74 (MQ=255)

                                                                                                       ggTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTcaaca                                                                               <  1:741915/74‑1 (MQ=255)

                                                                                                           aTAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTAGCCTTg                       >  1:1137308/1‑126 (MQ=255)

                                                                                                           aTAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTg                       <  2:1137308/126‑1 (MQ=255)

                                                                                                              aGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGa             >  2:1679740/1‑133 (MQ=255)

                                                                                                              aGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGa             <  1:1679740/133‑1 (MQ=255)

                                                                                                                   ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGc                                          >  2:218338/1‑99 (MQ=255)

                                                                                                                   ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGc                                          <  1:218338/99‑1 (MQ=255)

                                                                                                                   ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCaaa                                >  1:893108/1‑109 (MQ=255)

                                                                                                                   ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCaaa                                <  2:893108/109‑1 (MQ=255)

                                                                                                                   ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAg  >  1:1125488/1‑139 (MQ=255)

                                                                                                                     cTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATg                                                                 >  2:1666748/1‑74 (MQ=255)

                                                                                                                     cTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATg                                                                 <  1:1666748/74‑1 (MQ=255)

CATTGAAGAGGCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAG  >  NC_000913/2015963‑2016216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A