Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,016,095 |
G→A |
L76L (CTG→CTA) |
fliH → |
negative regulator of FliI ATPase activity |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,016,095 | 0 | G | A | 100.0%
| 74.2
/ NA
| 24 | L76L (CTG→CTA) | fliH | negative regulator of FliI ATPase activity |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/10); total (14/10) |
GCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCGTCGCGC > NC_000913/2015973‑2016224
|
gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGc > 1:678127/1‑139 (MQ=255)
gCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGc > 1:922294/1‑139 (MQ=255)
tGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCaa > 1:27971/1‑139 (MQ=255)
gAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAg > 1:1049124/1‑139 (MQ=255)
gAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAg > 2:1009570/1‑139 (MQ=255)
gCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGc > 2:228520/1‑139 (MQ=255)
gcagcaACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCg < 2:922294/139‑1 (MQ=255)
caACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGt > 2:860916/1‑139 (MQ=255)
gcgcAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTcaac < 1:1009570/139‑1 (MQ=255)
cccATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCTAAGTCTCAACAAGCGCCAATTCATGCCCg > 2:717851/1‑139 (MQ=255)
cccATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCg < 1:443293/139‑1 (MQ=255)
gggTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTcaaca < 1:1016275/99‑1 (MQ=255)
gggTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTcaaca > 2:1016275/1‑99 (MQ=255)
ccGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAAc > 2:928343/1‑139 (MQ=255)
ccAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCGAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGAt < 1:860916/139‑1 (MQ=255)
aGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGc < 2:678127/139‑1 (MQ=255)
gTCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCATTTGa < 1:440628/139‑1 (MQ=255)
tCATAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGAt > 2:933931/1‑139 (MQ=255)
aTAAGCAGGGCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAg < 1:228520/139‑1 (MQ=255)
ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCa < 2:1104978/123‑1 (MQ=255)
ggCTATCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCa > 1:1104978/1‑123 (MQ=255)
tCAGGAAGGACTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCGTcg > 1:475076/1‑139 (MQ=255)
aaggaCTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCGTCgcgc > 2:40237/1‑137 (MQ=255)
aaggaCTAGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCGTCgcgc < 1:40237/137‑1 (MQ=255)
|
GCTGAACCCAGCCTTGAGCAGCAACTGGCGCAACTGCAAATGCAGGCCCATGAGCAAGGTTATCAGGCGGGTATTGCCGAAGGTCGCCAGCAAGGTCATAAGCAGGGCTATCAGGAAGGACTGGCCCAGGGGCTGGAGCAAGGTCTGGCAGAGGCGAAGTCTCAACAAGCGCCAATTCATGCCCGGATGCAGCAACTGGTCAGCGAATTTCAAACTACCCTTGATGCACTTGATAGTGTGATAGCGTCGCGC > NC_000913/2015973‑2016224
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
