Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,350,162	A→G	L284L (TTG→CTG)	glpQ ←	periplasmic glycerophosphodiester phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,350,162	0	A	G	100.0%	169.5 / NA	48	L284L (TTG→CTG)	glpQ	periplasmic glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/24); total (24/24)

CAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAACATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGTCG  >  NC_000913/2350036‑2350295

cAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                           <  2:1233145/139‑1 (MQ=255)

cAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                           <  2:48131/139‑1 (MQ=255)

    aGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGa                                                                                                                       >  1:1042815/1‑139 (MQ=255)

        tATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCaa                                                                                                                   >  2:1487068/1‑139 (MQ=255)

           tCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                           >  1:1276643/1‑128 (MQ=255)

           tCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                           <  2:1276643/128‑1 (MQ=255)

           tCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTC‑ATCAGCATATGGTAATc                                                                                                                           <  2:1687673/127‑1 (MQ=255)

           tCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTC‑ATCAGCATATGGTAATc                                                                                                                           >  1:1687673/1‑127 (MQ=255)

                  gTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCg                                                                                                                         >  2:1478602/1‑123 (MQ=255)

                  gTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCg                                                                                                                         <  1:1478602/123‑1 (MQ=255)

                  gTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGc                                                                                                         >  1:812225/1‑139 (MQ=255)

                  gTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGc                                                                                                         >  2:1563708/1‑139 (MQ=255)

                    ttATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCat                                                                                                       <  1:1487068/139‑1 (MQ=255)

                           aCCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGc                                                                                                >  2:202986/1‑139 (MQ=255)

                            ccGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCa                                                                                                                                     <  1:306027/101‑1 (MQ=255)

                               cACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAcc                                                                                            >  2:1490803/1‑139 (MQ=255)

                                            tGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                               <  1:202986/139‑1 (MQ=255)

                                            tGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                               <  1:1563708/139‑1 (MQ=255)

                                            tGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGCTTGATATTACCCGGCTGCGATGTCTCCTCATTCAGCCTATGGTGATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                               <  2:262190/139‑1 (MQ=255)

                                              cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCa                                                                                                                    >  1:1626906/1‑100 (MQ=255)

                                              cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCa                                                                                                                    <  2:1626906/100‑1 (MQ=255)

                                              cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                             >  1:1133660/1‑139 (MQ=255)

                                              cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                             >  2:474717/1‑139 (MQ=255)

                                              cACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                             >  1:13134/1‑139 (MQ=255)

                                                    cAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTa                                                                       <  1:1490803/139‑1 (MQ=255)

                                                         tATTCTGTTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACAt                                                                  >  1:932105/1‑139 (MQ=255)

                                                              tgctgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGt                                                             <  1:474717/139‑1 (MQ=255)

                                                                ctgAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCCTCTGCATATGCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCg                                                           <  2:1042815/139‑1 (MQ=255)

                                                                                 tGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCa                                          >  2:1031131/1‑139 (MQ=255)

                                                                                 tGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCa                                          >  1:493519/1‑139 (MQ=255)

                                                                                  gCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGa                                                                                                                       <  1:1412986/61‑1 (MQ=255)

                                                                                  gCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGa                                                                                                                       >  2:1412986/1‑61 (MQ=255)

                                                                                     aGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                           >  2:1142479/1‑54 (MQ=255)

                                                                                     aGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATc                                                                                                                           <  1:1142479/54‑1 (MQ=255)

                                                                                     aGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCtt                                      <  2:1692751/139‑1 (MQ=255)

                                                                                                   tACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                             <  2:855850/86‑1 (MQ=255)

                                                                                                   tACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAccc                                                                             >  1:855850/1‑86 (MQ=255)

                                                                                                   tACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                               >  1:40149/1‑84 (MQ=255)

                                                                                                   tACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCAc                                                                               <  2:40149/84‑1 (MQ=255)

                                                                                                   tACCCGGCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGttt                        >  1:1375782/1‑139 (MQ=255)

                                                                                                        ggCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTctgct                   <  1:1031131/139‑1 (MQ=255)

                                                                                                         gCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATAc                                                                                                                <  1:324470/45‑1 (MQ=255)

                                                                                                         gCTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATAc                                                                                                                >  2:324470/1‑45 (MQ=255)

                                                                                                          cTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAc                                                                                             >  1:492104/1‑63 (MQ=255)

                                                                                                          cTGCGATGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCAc                                                                                             <  2:492104/63‑1 (MQ=255)

                                                                                                               aTGTCTCCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTc            >  1:179121/1‑139 (MQ=255)

                                                                                                                  tctcCTCAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCAtt         <  2:1375782/139‑1 (MQ=255)

                                                                                                                      ctcAATCAGCATATGGTAATCCGGACCAATACCATCTGCATATTCCACCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAg     <  2:812225/139‑1 (MQ=255)

                                                                                                                         aatcagCATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGTCg  >  2:1265987/1‑139 (MQ=255)

CAGGAGTGTATTCAGGCAGTTTATCTGACCGCACGGTATAAGGATGCACTACCAGTTTATTCTGCTGAGCATCTTGCACCATGCCAGTGAGTTTGATATTACCCGGCTGCGATGTCTCCTCAATCAACATATGGTAATCCGGACCAATACCATCTGCATATTCCGCCACCTGTTTCATGGCACCCGGCTTAAACATCCAGTCGTAGTTGTAATTAACCCAGCTTCCATCCGGCTGTTTCTGCTGCGTTTCATTCCAGTCG  >  NC_000913/2350036‑2350295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A