Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,313	G→A	R150C (CGT→TGT)	dapA ←	dihydrodipicolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,313	0	G	A	100.0%	144.6 / NA	45	R150C (CGT→TGT)	dapA	dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/24); total (21/24)

AAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGA  >  NC_000913/2599180‑2599434

aaGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCa                                                                                                                      <  1:1518824/139‑1 (MQ=255)

   tCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCg                                                                                                                   >  1:941774/1‑139 (MQ=255)

     cAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGtt                                                                                                                 <  1:340523/139‑1 (MQ=255)

     cAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGtt                                                                                                                 <  1:1510221/139‑1 (MQ=255)

      aGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGttt                                                                                                                >  2:730515/1‑139 (MQ=255)

      aGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGttt                                                                                                                >  2:1284111/1‑139 (MQ=255)

             tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAg                                                                                                         <  2:1360111/139‑1 (MQ=255)

                        ccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                              <  1:1284111/139‑1 (MQ=255)

                         cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                              >  1:82000/1‑138 (MQ=255)

                         cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                              <  2:82000/138‑1 (MQ=255)

                           cTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTc                                                                                                               >  2:608816/1‑119 (MQ=255)

                           cTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTc                                                                                                               <  1:608816/119‑1 (MQ=255)

                                    aCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                                                                                  <  1:555118/139‑1 (MQ=255)

                                             tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTc                                                                                                               <  1:118899/101‑1 (MQ=255)

                                             tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTc                                                                                                               >  2:118899/1‑101 (MQ=255)

                                             tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCACATCGCAGCCAGTACGGGACGGCACATtat                                                                         >  1:1146061/1‑139 (MQ=255)

                                              cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtata                                                                        >  2:629126/1‑139 (MQ=255)

                                                        ctctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                                                                    >  2:535786/1‑117 (MQ=255)

                                                        ctctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                                                                    <  1:535786/117‑1 (MQ=255)

                                                          ctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCgg                                                            <  2:941774/139‑1 (MQ=255)

                                                           tttGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGc                                                           >  2:351012/1‑139 (MQ=255)

                                                            ttGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACAGCACATTATACAGAATTTGCGGCa                                                          <  2:1146061/139‑1 (MQ=255)

                                                             tGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAg                                                         >  1:623040/1‑139 (MQ=255)

                                                             tGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAg                                                         >  1:1049941/1‑139 (MQ=255)

                                                                tCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc                                                      <  2:1049941/139‑1 (MQ=255)

                                                                tCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc                                                      <  2:623040/139‑1 (MQ=255)

                                                                     tttACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                              <  2:1406614/94‑1 (MQ=255)

                                                                     tttACACGCGTTAAGTTCCCTGTTGCCTCTATGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGc                                                                                              >  1:1406614/1‑94 (MQ=255)

                                                                              gTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGa                                        >  1:1040944/1‑139 (MQ=255)

                                                                               ttAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGAt                                       >  2:683802/1‑139 (MQ=255)

                                                                                        cTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                                                                    <  2:1489449/85‑1 (MQ=255)

                                                                                        cTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                                                                    >  1:1489449/1‑85 (MQ=255)

                                                                                            tGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGc                          <  1:683802/139‑1 (MQ=255)

                                                                                              ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATgg                                     <  1:891436/126‑1 (MQ=255)

                                                                                              ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATgg                                     >  2:891436/1‑126 (MQ=255)

                                                                                                 ctTTGATTCCGATAATATTTTTTACTTTCGCC‑‑ACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGa                                        >  2:371777/1‑118 (MQ=255)

                                                                                                 ctTTGATTCCGATAATATTTTTTACTTTCGCC‑‑ACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGa                                        <  1:371777/118‑1 (MQ=255)

                                                                                                     gATTCCGATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACaaa                 <  1:351012/139‑1 (MQ=255)

                                                                                                           gATAATATTTTTTACTTTCGCCAGACAGCCCATCGTTTCCGGGAGCAGATCGCAGCCAGTa                                                                                         >  2:1003678/1‑61 (MQ=255)

                                                                                                           gATAATATTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTa                                                                                         <  1:1003678/61‑1 (MQ=255)

                                                                                                            ataataTTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtat                                                                         <  2:1523675/76‑1 (MQ=255)

                                                                                                            ataataTTTTTTACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtat                                                                         >  1:1523675/1‑76 (MQ=255)

                                                                                                                    ttttACTTTCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga  <  2:1040944/139‑1 (MQ=255)

                                                                                                                            tCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCa                                                                                                <  1:1076479/37‑1 (MQ=255)

                                                                                                                            tCGCCAGACAGCCCACCGTTTCCGGGAGCAGATCGCa                                                                                                >  2:1076479/1‑37 (MQ=255)

AAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGA  >  NC_000913/2599180‑2599434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A