Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,655,767	T→C	T198A (ACT→GCT)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,655,767	0	T	C	100.0%	229.5 / NA	63	T198A (ACT→GCT)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/33); total (30/33)

GCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTG  >  NC_000913/2655632‑2655896

gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                >  1:1199769/1‑139 (MQ=255)

gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                >  2:1342332/1‑139 (MQ=255)

gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                <  1:1650864/139‑1 (MQ=255)

gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                >  2:1348286/1‑139 (MQ=255)

 cTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATa                                                                                                                               >  2:89485/1‑139 (MQ=255)

     aCGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCtt                                                                                                                           <  1:1420322/139‑1 (MQ=255)

       gcgcTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTta                                                                                                                         <  1:43696/139‑1 (MQ=255)

        cgcTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTtac                                                                                                                        <  1:161637/139‑1 (MQ=255)

             gTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTtacctacc                                                                                                                   >  2:824859/1‑139 (MQ=255)

                   tCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAc                                                                                                             <  2:28587/139‑1 (MQ=255)

                     cgcgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAcgc                                                                                                           <  1:239382/139‑1 (MQ=255)

                       cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGt                                                                                                         >  2:704917/1‑139 (MQ=255)

                             aTGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTg                                                                                                   >  2:712486/1‑139 (MQ=255)

                             aTGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTg                                                                                                   >  2:1221816/1‑139 (MQ=255)

                                gCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGgcg                                                                                                <  2:957727/139‑1 (MQ=255)

                                          gcgcCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCTATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATc                                                                                      <  2:1199769/139‑1 (MQ=255)

                                          gcgcCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                >  1:1239446/1‑113 (MQ=255)

                                          gcgcCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                <  2:1239446/113‑1 (MQ=255)

                                           cgcCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCg                                                                                     >  1:312809/1‑139 (MQ=255)

                                            gcCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGc                                                                                    <  2:168503/139‑1 (MQ=255)

                                                 ggTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGtt                                                                               <  2:312809/139‑1 (MQ=255)

                                                 ggTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGtt                                                                               <  2:1068393/139‑1 (MQ=255)

                                                    aaCCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTggg                                                                            <  2:309248/139‑1 (MQ=255)

                                                            ccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAc                                                                                                             <  2:107309/98‑1 (MQ=255)

                                                            ccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAc                                                                                                             >  1:107309/1‑98 (MQ=255)

                                                              gcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCa                                                                  <  1:1672527/139‑1 (MQ=255)

                                                                       aTGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCa                                                         <  2:309447/139‑1 (MQ=255)

                                                                                 tCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                               <  1:704917/139‑1 (MQ=255)

                                                                                 tCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                               <  1:1221816/139‑1 (MQ=255)

                                                                                   aTCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAg                                                           <  1:866821/125‑1 (MQ=255)

                                                                                   aTCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAg                                                           >  2:866821/1‑125 (MQ=255)

                                                                                   aTCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAAc                                             >  1:86994/1‑139 (MQ=255)

                                                                                                 cGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGtt                                                                               <  1:657427/91‑1 (MQ=255)

                                                                                                 cGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGtt                                                                               >  2:657427/1‑91 (MQ=255)

                                                                                                   cAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACCCTGGCGCTTCTTTATCGCCAGt                                                                                <  2:175541/88‑1 (MQ=255)

                                                                                                   cAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGt                                                                                >  1:175541/1‑88 (MQ=255)

                                                                                                   cAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTcc                             >  2:205573/1‑139 (MQ=255)

                                                                                                     gTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                >  1:457195/1‑54 (MQ=255)

                                                                                                     gTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                <  2:457195/54‑1 (MQ=255)

                                                                                                     gTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGa                           >  2:1631016/1‑139 (MQ=255)

                                                                                                      tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGt                                                                                >  1:797943/1‑85 (MQ=255)

                                                                                                      tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGt                                                                                <  2:797943/85‑1 (MQ=255)

                                                                                                       cTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                         <  1:1348286/139‑1 (MQ=255)

                                                                                                        tGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAc                                                                                                             <  1:1537131/54‑1 (MQ=255)

                                                                                                        tGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAc                                                                                                             >  2:1537131/1‑54 (MQ=255)

                                                                                                          tttGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtg                      >  1:462465/1‑139 (MQ=255)

                                                                                                            tGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTta                                                                                                                         <  2:1525982/38‑1 (MQ=255)

                                                                                                            tGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTta                                                                                                                         >  1:1525982/1‑38 (MQ=255)

                                                                                                            tGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                    <  1:89485/139‑1 (MQ=255)

                                                                                                               tGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                 <  1:824859/139‑1 (MQ=255)

                                                                                                                gCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCATGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCgg                                                  <  1:1315628/105‑1 (MQ=255)

                                                                                                                gCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCATGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCgg                                                  >  2:1315628/1‑105 (MQ=255)

                                                                                                                gCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCt                                        >  2:1274374/1‑115 (MQ=255)

                                                                                                                gCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCt                                        <  1:1274374/115‑1 (MQ=255)

                                                                                                                    tAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCa            >  1:1275584/1‑139 (MQ=255)

                                                                                                                      gccgccGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGcc                                                             <  2:1151942/88‑1 (MQ=255)

                                                                                                                      gccgccGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGcc                                                             >  1:1151942/1‑88 (MQ=255)

                                                                                                                         gccgGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATaa       >  2:730573/1‑139 (MQ=255)

                                                                                                                            ggAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAAcc                                   <  1:1365864/108‑1 (MQ=255)

                                                                                                                            ggAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAAcc                                   >  2:1365864/1‑108 (MQ=255)

                                                                                                                              aGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAAcc                                   <  2:112722/106‑1 (MQ=255)

                                                                                                                              aGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAAcc                                   >  1:112722/1‑106 (MQ=255)

                                                                                                                              aGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTg  >  2:1264327/1‑139 (MQ=255)

GCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTG  >  NC_000913/2655632‑2655896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A