Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,655,767	T→C	T198A (ACT→GCT)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,655,767	0	T	C	100.0%	159.8 / NA	45	T198A (ACT→GCT)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/23); total (22/23)

AGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTG  >  NC_000913/2655631‑2655896

aGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGCTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGGCGGAGTCAAAAGCGa                                                                                                                                 <  2:898655/139‑1 (MQ=255)

 gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                >  2:720100/1‑139 (MQ=255)

 gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                >  1:10302/1‑139 (MQ=255)

 gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                <  1:720100/139‑1 (MQ=255)

 gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGAt                                                                                                                                >  2:402320/1‑139 (MQ=255)

        gcgcTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTta                                                                                                                         <  1:402320/139‑1 (MQ=255)

        gcgcTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTta                                                                                                                         <  2:32989/139‑1 (MQ=255)

                        cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGt                                                                                                         >  2:439592/1‑139 (MQ=255)

                         gcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTa                                                                                                        >  1:253642/1‑139 (MQ=255)

                              aTGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                <  1:285625/126‑1 (MQ=255)

                              aTGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                                >  2:285625/1‑126 (MQ=255)

                                          aGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTAt                                                                                       <  1:439592/139‑1 (MQ=255)

                                                          ttGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTtaccta                                                                                                                     <  2:910811/93‑1 (MQ=255)

                                                          ttGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTtaccta                                                                                                                     >  1:910811/1‑93 (MQ=255)

                                                           tGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTaa                                                                      >  2:1220525/1‑139 (MQ=255)

                                                             ccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                    >  1:508316/1‑139 (MQ=255)

                                                                 gccgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgc                                                                <  2:253642/139‑1 (MQ=255)

                                                                  ccgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcg                                                               >  1:1341695/1‑139 (MQ=255)

                                                                  ccgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCGACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcg                                                               <  1:992667/139‑1 (MQ=255)

                                                                           tCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATa                                                      <  2:508316/139‑1 (MQ=255)

                                                                             cGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATAcc                                                    <  2:10302/139‑1 (MQ=255)

                                                                                  tCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                               <  1:1220525/139‑1 (MQ=255)

                                                                                                   gCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTc                              <  2:1120479/139‑1 (MQ=255)

                                                                                                    cAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCa                                                                  >  2:329740/1‑102 (MQ=255)

                                                                                                    cAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCa                                                                  <  1:329740/102‑1 (MQ=255)

                                                                                                       tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcg                                                               >  2:325124/1‑102 (MQ=255)

                                                                                                       tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcg                                                               <  1:325124/102‑1 (MQ=255)

                                                                                                        cTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATAACTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                         <  2:1278119/139‑1 (MQ=255)

                                                                                                           tttGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                               >  1:569844/1‑114 (MQ=255)

                                                                                                           tttGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                               <  2:569844/114‑1 (MQ=255)

                                                                                                                tGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                 <  2:1126985/139‑1 (MQ=255)

                                                                                                                 gCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgc                                                                >  1:863765/1‑91 (MQ=255)

                                                                                                                 gCTGTAGCCGCCGGAATCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgc                                                                <  2:863765/91‑1 (MQ=255)

                                                                                                                     tAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGCCTGTGTGCAGCCCCa            >  1:178925/1‑139 (MQ=255)

                                                                                                                     tAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCa            >  2:973691/1‑139 (MQ=255)

                                                                                                                     tAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCa            >  1:703871/1‑139 (MQ=255)

                                                                                                                       gccgccGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                <  2:568706/133‑1 (MQ=255)

                                                                                                                       gccgccGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                >  1:568706/1‑133 (MQ=255)

                                                                                                                          gccgGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGcc                                                             >  2:823328/1‑85 (MQ=255)

                                                                                                                          gccgGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGcc                                                             <  1:823328/85‑1 (MQ=255)

                                                                                                                          gccgGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATaa       >  2:933489/1‑139 (MQ=255)

                                                                                                                           ccgGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAAc      <  1:1214835/139‑1 (MQ=255)

                                                                                                                               aGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcg                                                               <  2:1041678/78‑1 (MQ=255)

                                                                                                                               aGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgcg                                                               >  1:1041678/1‑78 (MQ=255)

                                                                                                                               aGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTg  >  2:402189/1‑139 (MQ=255)

AGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTG  >  NC_000913/2655631‑2655896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A