Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
759 |
65.2 |
3386260 |
90.7% |
3071337 |
101.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,330,396 |
G→A |
*478* (TAG→TAA) |
dacB → |
D‑alanyl‑D‑alanine carboxypeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,330,396 | 0 | G | A | 100.0%
| 109.6
/ NA
| 35 | *478* (TAG→TAA) | dacB | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/17); total (18/17) |
TCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCA > NC_000913/3330294‑3330510
|
tCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCa > 2:293161/1‑139 (MQ=255)
ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac‑a < 2:950250/122‑1 (MQ=255)
ttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac‑a > 1:950250/1‑122 (MQ=255)
aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACaa < 2:1418910/133‑1 (MQ=255)
aGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACaa > 1:1418910/1‑133 (MQ=255)
gAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTgcgc < 1:293161/139‑1 (MQ=255)
aaCCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt < 2:275267/139‑1 (MQ=255)
ccTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCttt > 1:1300649/1‑139 (MQ=255)
tGCGGATCAGCGTAAACGCCATATTCCGTTAGTGCGTTTTTGAAACCCATTGGTAAAAGGAAATTTATCAGAACAATAAAT‑AAAAAAAAAACCCAGCACAATGGCCGGGGCAGCAGATAATTAACaaaatcagcttaga > 2:56998/1‑128 (MQ=255)
gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCg < 2:937484/106‑1 (MQ=255)
gCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCg > 1:937484/1‑106 (MQ=255)
ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGt > 2:566919/1‑127 (MQ=255)
ggATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGt < 1:566919/127‑1 (MQ=255)
gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac < 1:1692558/80‑1 (MQ=255)
gTATTCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac > 2:1692558/1‑80 (MQ=255)
ttCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt < 1:1415803/113‑1 (MQ=255)
ttCCGTTAGTGCGTTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCtt > 2:1415803/1‑113 (MQ=255)
cgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTGATCAAAAAGa > 2:1198839/4‑55 (MQ=255)
cgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGa < 1:1198839/52‑1 (MQ=255)
cgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGatta < 2:1023465/83‑1 (MQ=255)
cgtttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGatta > 1:1023465/4‑86 (MQ=255)
tttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCa > 1:778556/1‑139 (MQ=255)
ttGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaa > 2:418710/1‑139 (MQ=255)
tGTATAAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa < 1:418710/139‑1 (MQ=255)
taAAGATATTTATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa < 2:778556/139‑1 (MQ=255)
tatTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac > 1:603170/1‑39 (MQ=38)
tatTTATCAGAACAATTAATCAAAAAGAAACCCCGGcac < 2:603170/39‑1 (MQ=38)
tttATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTc > 2:29169/1‑51 (MQ=255)
tttATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTc < 1:29169/51‑1 (MQ=255)
tttATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg < 2:1407600/88‑1 (MQ=255)
tttATCAGAACAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATg > 1:1407600/1‑88 (MQ=255)
aaCAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTc > 2:228560/1‑43 (MQ=255)
aaCAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTc < 1:228560/43‑1 (MQ=255)
cAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt < 1:686184/62‑1 (MQ=255)
cAATTAATCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt > 2:686184/1‑62 (MQ=255)
|
TCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCG‑TTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCAC‑ATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCA > NC_000913/3330294‑3330510
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
