Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I1 R1 759 65.2 3386260 90.7% 3071337 101.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,609,336 (C)7→8 intergenic (+13/‑78) yjjG → / → prfC dUMP phosphatase/peptide chain release factor RF‑3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,609,3291.C100.0% 123.0 / NA 36intergenic (+6/‑85)yjjG/prfCdUMP phosphatase/peptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (19/17);  total (19/17)

TCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTT  >  NC_000913/4609193‑4609461
                                                                                                                                         |                                                                                                                                     
tcGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cc                                                                                                                                    <  1:1615712/139‑1 (MQ=255)
    tGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cccccc                                                                                                                                <  1:1012695/139‑1 (MQ=255)
            aCGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          >  1:434177/1‑132 (MQ=255)
            aCGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  2:434177/137‑6 (MQ=255)
                                                aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCCCGTTGATGGGTAAAATAg                                                                                                              <  1:54860/115‑1 (MQ=255)
                                                aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCCCGTTGATGGGTAAAATAg                                                                                                              >  2:54860/1‑115 (MQ=255)
                                                aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGtt                                                                                               <  1:815887/129‑1 (MQ=255)
                                                aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGtt                                                                                               >  2:815887/1‑129 (MQ=255)
                                                aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cccc                                                                                                                                  >  2:484531/1‑93 (MQ=255)
                                                aTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cccc                                                                                                                                  <  1:484531/93‑1 (MQ=255)
                                                 tCGCGAGCAACCAGACGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cc                                                                                                                                    >  2:1132231/1‑90 (MQ=255)
                                                 tCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGCTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cc                                                                                                                                    <  1:1132231/90‑1 (MQ=255)
                                                                aGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt                                                                     <  1:820105/139‑1 (MQ=255)
                                                                   cATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg                                                                                    <  2:1557209/121‑1 (MQ=255)
                                                                   cATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCAGCAATTTTTCGTTTTCAACAAgcg                                                                                    >  1:1557209/1‑121 (MQ=255)
                                                                     tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt                                                                     <  1:1253929/134‑1 (MQ=255)
                                                                     tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt                                                                     >  2:1253929/1‑134 (MQ=255)
                                                                     tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc                                                                >  1:260449/1‑139 (MQ=255)
                                                                     tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTc                                                                >  1:599674/1‑139 (MQ=255)
                                                                      cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCa                                                               >  2:320562/1‑139 (MQ=255)
                                                                                       tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaag                                                             <  1:302900/124‑1 (MQ=255)
                                                                                       tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaag                                                             >  2:302900/1‑124 (MQ=255)
                                                                                       tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa                                              >  2:781391/1‑139 (MQ=255)
                                                                                       tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGa                                              >  1:1239943/1‑139 (MQ=255)
                                                                                       tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          >  1:1116152/1‑57 (MQ=255)
                                                                                       tttCTTCATTGCACGAACTGGACCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  2:1116152/62‑6 (MQ=255)
                                                                                        ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  1:119173/61‑6 (MQ=255)
                                                                                        ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          >  2:119173/1‑56 (MQ=255)
                                                                                         tcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg                                            <  2:260449/139‑1 (MQ=255)
                                                                                              gTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGtc                                       <  1:138421/139‑1 (MQ=255)
                                                                                                             gcagcTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAaga                        <  1:781391/139‑1 (MQ=255)
                                                                                                                 cTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                           <  1:1147593/132‑1 (MQ=255)
                                                                                                                 cTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                           >  2:1147593/1‑132 (MQ=255)
                                                                                                                     tgtgTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                                                         <  2:1628593/98‑1 (MQ=255)
                                                                                                                     tgtgTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                                                         >  1:1628593/1‑98 (MQ=255)
                                                                                                                           aaCACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGcc                                                                        >  2:1532530/1‑77 (MQ=255)
                                                                                                                           aaCACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGcc                                                                        <  1:1532530/77‑1 (MQ=255)
                                                                                                                           aaCACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGcgcc          >  2:35567/1‑139 (MQ=255)
                                                                                                                            acacTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGcgccg         <  1:35567/139‑1 (MQ=255)
                                                                                                                            acacTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGcgccg         <  1:320562/139‑1 (MQ=255)
                                                                                                                              acTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCa       >  2:394255/1‑139 (MQ=255)
                                                                                                                                 gATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAAttttt                                                                                                   <  2:5483/44‑1 (MQ=38)
                                                                                                                                 gATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAAttttt                                                                                                   >  1:5483/1‑44 (MQ=38)
                                                                                                                                 gATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                                                         >  2:1157005/1‑86 (MQ=255)
                                                                                                                                 gATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga                                                         <  1:1157005/86‑1 (MQ=255)
                                                                                                                                   ttGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa                                 <  2:641522/108‑1 (MQ=255)
                                                                                                                                   ttGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa                                 >  1:641522/1‑108 (MQ=255)
                                                                                                                                   ttGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACtttt  >  1:247811/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                     
TCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTT  >  NC_000913/4609193‑4609461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A