Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 757 76.1 3598070 93.4% 3360597 108.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,609,336 (C)7→8 intergenic (+13/‑78) yjjG → / → prfC dUMP phosphatase/peptide chain release factor RF‑3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,609,3291.C100.0% 186.5 / NA 52intergenic (+6/‑85)yjjG/prfCdUMP phosphatase/peptide chain release factor RF‑3
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (27/25);  total (27/25)

TCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTT  >  NC_000913/4609193‑4609461
                                                                                                                                         |                                                                                                                                     
tcGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cc                                                                                                                                    >  2:1498461/1‑139 (MQ=255)
     ggCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccc                                                                                                                               >  2:509438/1‑139 (MQ=255)
       cATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGTAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccc                                                                                                                             >  2:1132115/1‑137 (MQ=255)
         tCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgtt                                                                                                                           >  1:349757/1‑135 (MQ=255)
          cAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  1:1145035/139‑6 (MQ=255)
          cAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  1:515906/139‑6 (MQ=255)
           aaCGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCCCGAGTAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cccccccccgttg                                                                                                                         <  1:1132115/139‑7 (MQ=255)
                cGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCATCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGt                                                                                                                     >  1:1781463/1‑139 (MQ=255)
                 gggCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTa                                                                                                                    >  2:427686/1‑139 (MQ=255)
                 gggCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTa                                                                                                                    >  2:519140/1‑139 (MQ=255)
                      tGCGACCTGCTGGCTCAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGATCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATa                                                                                                               >  1:1200339/1‑139 (MQ=255)
                       gCGACCTGCTGGCTGTATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAg                                                                                                              >  1:1199007/1‑139 (MQ=255)
                       gCGACCTGCTGGCTGAATGCACACCCTCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAg                                                                                                              >  1:868227/1‑139 (MQ=255)
                            ctgctgGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCa                                                                                                         >  1:377350/1‑139 (MQ=255)
                               ctggctgAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  1:816757/118‑6 (MQ=255)
                               ctggctgAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          >  2:816757/1‑113 (MQ=255)
                                         gCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTc                                                                                            >  1:1003996/1‑139 (MQ=255)
                                            cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGcc                                                                                                            >  1:1410469/1‑120 (MQ=255)
                                            cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGcc                                                                                                            <  2:1410469/120‑1 (MQ=255)
                                            cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaac                                                                                         >  2:649217/1‑139 (MQ=255)
                                            cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaac                                                                                         <  2:1199007/139‑1 (MQ=255)
                                            cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  2:1155994/105‑6 (MQ=255)
                                            cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          >  1:1155994/1‑100 (MQ=255)
                                            cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCCTTTCTTCGTTGCACGAACTGGAGCAGCTCCTATGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaac                                                                                         <  2:868227/139‑1 (MQ=255)
                                             acCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaac                                                                                         <  2:1597365/138‑1 (MQ=255)
                                             acCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTcaac                                                                                         >  1:1597365/1‑138 (MQ=255)
                                                   gcgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcg                                                                                  <  1:381370/139‑1 (MQ=255)
                                                    cgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cccccc                                                                                                                                >  1:227125/1‑91 (MQ=255)
                                                    cgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cccccc                                                                                                                                <  2:227125/91‑1 (MQ=255)
                                                          aCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt                                                                           <  1:519140/139‑1 (MQ=255)
                                                           ccAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTCCCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt                                                                           <  1:1498461/139‑1 (MQ=255)
                                                           ccAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATg                                                                          >  1:1522054/1‑139 (MQ=255)
                                                           ccAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATg                                                                          >  1:1179760/1‑139 (MQ=255)
                                                                   cATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTAc                                                                  >  2:200355/1‑139 (MQ=255)
                                                                     tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg                                                                                    >  2:358881/1‑119 (MQ=255)
                                                                     tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcg                                                                                    <  1:358881/119‑1 (MQ=255)
                                                                     tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  2:326389/80‑6 (MQ=255)
                                                                     tCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          >  1:326389/1‑75 (MQ=255)
                                                                      cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCa                                                               >  2:201355/1‑139 (MQ=255)
                                                                      cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cccc                                                                                                                                  <  1:1171039/71‑1 (MQ=255)
                                                                      cgcgCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑cccc                                                                                                                                  >  2:1171039/1‑71 (MQ=255)
                                                                       gcgcCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaa                                                              <  2:349757/139‑1 (MQ=255)
                                                                       gcgcCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  1:1715503/78‑6 (MQ=255)
                                                                       gcgcCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          >  2:1715503/1‑73 (MQ=255)
                                                                        cgcCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaag                                                             >  2:1748282/1‑139 (MQ=255)
                                                                            cACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAATCGCGGCGCGATGCCGCTTACTCAagaaga                                                         <  2:1200339/139‑1 (MQ=255)
                                                                             aCCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAgaagaa                                                        <  1:858567/139‑1 (MQ=255)
                                                                                       tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGTAAGAATTATGa                                              >  1:250105/1‑139 (MQ=255)
                                                                                       tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  1:858183/62‑6 (MQ=255)
                                                                                       tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          >  2:858183/1‑57 (MQ=255)
                                                                                        ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          >  2:1402302/1‑56 (MQ=255)
                                                                                        ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑ccccccccgttg                                                                                                                          <  1:1402302/61‑6 (MQ=255)
                                                                                         tcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg                                            <  2:1781463/139‑1 (MQ=255)
                                                                                           ttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGTAAGAATTATGACGtt                                          <  2:250105/139‑1 (MQ=255)
                                                                                            tcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTg                                         >  1:1582482/1‑139 (MQ=255)
                                                                                               ttGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCg                                                                                                 <  1:1113146/80‑1 (MQ=255)
                                                                                               ttGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCg                                                                                                 >  2:1113146/1‑80 (MQ=255)
                                                                                                    cGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa                                 <  1:427686/139‑1 (MQ=255)
                                                                                                       aCTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt                                                                     >  2:522932/1‑100 (MQ=255)
                                                                                                       aCTGGAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCt                                                                     <  1:522932/100‑1 (MQ=255)
                                                                                                          gggaggcaatcagtgtAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                           <  2:248538/127‑1 (MQ=255)
                                                                                                          ggcgcagcTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                           <  1:1189110/136‑1 (MQ=255)
                                                                                                          ggAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                           <  2:1492493/139‑1 (MQ=255)
                                                                                                          ggAGCAGCTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                           <  1:442785/139‑1 (MQ=255)
                                                                                                                 cTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGa                                                                                                                          <  2:1558582/37‑1 (MQ=255)
                                                                                                                 cTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGa                                                                                                                          >  1:1558582/1‑37 (MQ=255)
                                                                                                                 cTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAGATAGCCGCGATTTTTCGATGTCAAGAATCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGCATTGTGACGTTGTCTCCTTATTTGCAAGAGGTg                    >  1:836399/1‑139 (MQ=255)
                                                                                                                 cTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                           <  1:681783/132‑1 (MQ=255)
                                                                                                                 cTCCTGTGTAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCa                           >  2:681783/1‑132 (MQ=255)
                                                                                                                         tAAACACTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAgcg            <  1:649217/139‑1 (MQ=255)
                                                                                                                              acTGATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCa       >  2:1663188/1‑139 (MQ=255)
                                                                                                                                 gATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaaga                                                            <  1:856293/83‑1 (MQ=255)
                                                                                                                                 gATTGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCaaga                                                            >  2:856293/1‑83 (MQ=255)
                                                                                                                                   ttGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACtttt  <  1:1663188/139‑1 (MQ=255)
                                                                                                                                   ttGCCTC‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACtttt  >  2:104626/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                     
TCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCCT‑‑CCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCGCACTTTT  >  NC_000913/4609193‑4609461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A