Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	866,518	T→C	intergenic (‑154/‑50)	moeA ← / → iaaA	molybdopterin molybdenumtransferase, molybdopterin biosynthesis protein/Isoaspartyl peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	866,518	0	T	C	100.0%	135.2 / NA	38	intergenic (‑154/‑50)	moeA/iaaA	molybdopterin molybdenumtransferase, molybdopterin biosynthesis protein/Isoaspartyl peptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/20); total (18/20)

AAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAAT  >  NC_000913/866385‑866643

aaaaTCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGa                                                                                                                          >  2:209451/1‑139 (MQ=255)

  aaTCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGata                                                                                                                        >  1:917696/1‑139 (MQ=255)

    tCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATAtt                                                                                                                      <  2:1010178/139‑1 (MQ=255)

                 ttatGTCCGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTg                                                                                                         <  2:1087275/139‑1 (MQ=255)

                       cAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   >  1:916030/1‑139 (MQ=255)

                        aGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAacc                                                                                                  <  2:916030/139‑1 (MQ=255)

                          aaaaaCGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAaccac                                                                                                >  1:20675/1‑139 (MQ=255)

                                  cacaGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATg                                                                                        <  2:20675/139‑1 (MQ=255)

                                  cacaGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATg                                                                                        <  2:917696/139‑1 (MQ=255)

                                       aCTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                                          >  2:455117/1‑132 (MQ=255)

                                       aCTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                                          <  1:455117/132‑1 (MQ=255)

                                                     cGGGCGTAGCGCTGGGTCCTGTCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   <  1:175459/109‑1 (MQ=255)

                                                     cGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGaa                                                                                                    >  2:579845/1‑108 (MQ=255)

                                                     cGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGaa                                                                                                    <  1:579845/108‑1 (MQ=255)

                                                     cGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   >  2:175459/1‑109 (MQ=255)

                                                       ggCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   >  1:276078/1‑107 (MQ=255)

                                                       ggCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAac                                                                                                   <  2:276078/107‑1 (MQ=255)

                                                                             ttACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAACAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGgc                                             >  1:424813/1‑139 (MQ=255)

                                                                              tACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGaa                                                                                                    <  1:985751/83‑1 (MQ=255)

                                                                              tACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGaa                                                                                                    >  2:985751/1‑83 (MQ=255)

                                                                              tACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGgcg                                            <  1:209451/139‑1 (MQ=255)

                                                                                              cTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAacc                                                                                                  >  1:54994/1‑69 (MQ=255)

                                                                                              cTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAacc                                                                                                  <  2:54994/69‑1 (MQ=255)

                                                                                                 tCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCAtt                                                            <  1:119193/104‑1 (MQ=255)

                                                                                                 tCTATATTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCAtt                                                            >  2:119193/1‑104 (MQ=255)

                                                                                                      atTCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGa                    <  1:566030/139‑1 (MQ=255)

                                                                                                       ttCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTg                                                           >  1:108667/1‑99 (MQ=255)

                                                                                                       ttCAAAAATTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTg                                                           <  2:108667/99‑1 (MQ=255)

                                                                                                             aaTTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                                          <  2:1018720/62‑1 (MQ=255)

                                                                                                             aaTTGAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTaa                                                                                          >  1:1018720/1‑62 (MQ=255)

                                                                                                                 gAATGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCaa         >  1:770238/1‑139 (MQ=255)

                                                                                                                    tGAGTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAg      >  1:293748/1‑139 (MQ=255)

                                                                                                                       gTAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGaa   <  1:15555/139‑1 (MQ=255)

                                                                                                                        tAATTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAAt  <  2:424813/139‑1 (MQ=255)

                                                                                                                         aaTTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGcc                             >  2:509356/1‑111 (MQ=255)

                                                                                                                         aaTTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGcc                             <  1:509356/111‑1 (MQ=255)

                                                                                                                          aTTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATg                                                                                        >  2:415610/1‑51 (MQ=255)

                                                                                                                          aTTCATAAAAACTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATg                                                                                        <  1:415610/51‑1 (MQ=255)

AAAATCGAATTTGCCTATTATGTCAGAAAAACGCCACAGACTGTATGCCACCTCGGGCGTAGCGCTGGGTCCTGCCTTTACATGCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAAT  >  NC_000913/866385‑866643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A