Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,892,402	A→G	S278S (AGT→AGC)	yoaA ←	putative ATP‑dependent helicase, DinG family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,892,402	0	A	G	100.0%	122.4 / NA	35	S278S (AGT→AGC)	yoaA	putative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/17); total (18/17)

TCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGG  >  NC_000913/1892269‑1892535

tCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTg                                                                                                                                  >  2:281543/1‑139 (MQ=255)

      cGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAg                                                                                                                            >  1:19959/1‑139 (MQ=255)

      cGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAg                                                                                                                            <  2:413762/139‑1 (MQ=255)

                 aGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                 <  1:227878/139‑1 (MQ=255)

                 aGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                 <  2:466327/139‑1 (MQ=255)

                   ttCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTCGCTAACAGCTCACGCCGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgcgc                                                                                                               >  2:953033/1‑137 (MQ=255)

                             tCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAAct                                                                                                     >  2:43324/1‑139 (MQ=255)

                                   aGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTggg                                                                                               >  2:358151/1‑139 (MQ=255)

                                            aaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTa                                                                                      <  1:972672/139‑1 (MQ=255)

                                            aaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTa                                                                                      <  1:365893/139‑1 (MQ=255)

                                                  cGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                                                  >  2:821087/1‑105 (MQ=255)

                                                  cGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcg                                                                                                                  <  1:821087/105‑1 (MQ=255)

                                                      gAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCg                                                                            >  2:990742/1‑139 (MQ=255)

                                                        aTTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGt                                                                          <  1:43324/139‑1 (MQ=255)

                                                                          aGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAAc                                                                                                      <  1:295416/93‑1 (MQ=255)

                                                                          aGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAAc                                                                                                      >  2:295416/1‑93 (MQ=255)

                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                 <  2:430464/80‑1 (MQ=255)

                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                 <  2:1068683/80‑1 (MQ=255)

                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                 >  1:1068683/1‑80 (MQ=255)

                                                                            cTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                 >  1:430464/1‑80 (MQ=255)

                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgc                                                                                                                   <  2:228980/76‑1 (MQ=255)

                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAgc                                                                                                                   >  1:228980/1‑76 (MQ=255)

                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCg                                                                            <  1:757313/115‑1 (MQ=255)

                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCg                                                                            >  2:757313/1‑115 (MQ=255)

                                                                              cACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGc                                                    >  2:973047/1‑139 (MQ=255)

                                                                               aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGcc                                                   <  1:990742/139‑1 (MQ=255)

                                                                                                 aCCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGa                                 <  1:953033/139‑1 (MQ=255)

                                                                                                            cGAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtga                      >  1:665200/1‑139 (MQ=255)

                                                                                                             gAGTTGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGtgac                     >  1:473559/1‑139 (MQ=255)

                                                                                                                ttGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGa                                                              >  1:127077/1‑95 (MQ=255)

                                                                                                                ttGCAGACGAAAATCCTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGa                                                              <  2:127077/95‑1 (MQ=255)

                                                                                                                        gAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                 <  1:271870/36‑1 (MQ=255)

                                                                                                                        gAAAATCCTGCGCGCTCTGGGCAAGACGATCAgcgc                                                                                                                 >  2:271870/1‑36 (MQ=255)

                                                                                                                              ccTGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActggct    >  2:208394/1‑139 (MQ=255)

                                                                                                                                tGCGCGCTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATActggctgg  <  2:665200/139‑1 (MQ=255)

TCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTGGAGAGTGACTGACCAAAATACTGGCTGG  >  NC_000913/1892269‑1892535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A