Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,926,096	G→A	219 (TAG→TAA)	yobB →	C‑N hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,096	0	G	A	100.0%	184.3 / NA	58	219 (TAG→TAA)	yobB	C‑N hydrolase family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/27); total (31/27)

TAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925976‑1926229

tAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGc                                                                                                                     <  1:158208/139‑1 (MQ=255)

   cAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATg                                                                                                                  >  2:37633/1‑139 (MQ=255)

     gTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  2:189556/1‑128 (MQ=255)

     gTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:189556/128‑1 (MQ=255)

        cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             <  2:241607/139‑1 (MQ=255)

        cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                             >  1:241607/1‑139 (MQ=255)

         aCTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGt                                                                                                            >  1:1047466/1‑139 (MQ=255)

              gggATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       <  1:928496/139‑1 (MQ=255)

                gATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAt                                                                                                     >  1:262735/1‑139 (MQ=255)

                  tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  2:606008/1‑115 (MQ=255)

                  tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:606008/115‑1 (MQ=255)

                       atGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       >  1:1017993/1‑130 (MQ=255)

                       atGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                       <  2:1017993/130‑1 (MQ=255)

                              tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGaaa                                                                                       >  1:108595/1‑139 (MQ=255)

                               ctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                      >  2:996379/1‑139 (MQ=255)

                               ctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                      >  1:384141/1‑139 (MQ=255)

                                    cGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCg                                                                                 <  2:1047466/139‑1 (MQ=255)

                                       tCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGtt                                                                              >  2:1085044/1‑139 (MQ=255)

                                                tCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                     <  1:1085044/139‑1 (MQ=255)

                                                 cGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggagg                                                                    <  1:385360/139‑1 (MQ=255)

                                                             ttGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                        <  2:102357/139‑1 (MQ=255)

                                                                ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAg                                                                                                                      <  1:344275/74‑1 (MQ=255)

                                                                ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAg                                                                                                                      >  2:344275/1‑74 (MQ=255)

                                                                  aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  <  1:236653/92‑1 (MQ=255)

                                                                  aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                  >  2:236653/1‑92 (MQ=255)

                                                                  aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   >  2:509162/1‑139 (MQ=255)

                                                                   gTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                >  2:250581/1‑77 (MQ=255)

                                                                   gTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                <  1:250581/77‑1 (MQ=255)

                                                                     cGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATCCCATTACGCTAAGCGTTTTGCGACTGGAGCATGTCATGTTGCGCATTATCGATACAGAAACCTGCGGTGTGCAGGGAGGGTACGTTGATATTGCCTCTg                                                >  2:188353/1‑139 (MQ=255)

                                                                       gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGtt                                              >  2:119262/1‑139 (MQ=255)

                                                                        tCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGC‑TTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGa                                            >  1:351373/1‑139 (MQ=255)

                                                                                 atcaCAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGC‑TTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGa                                   <  2:351373/139‑1 (MQ=255)

                                                                                        ggTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaa                             >  1:229003/1‑139 (MQ=255)

                                                                                           tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                >  1:1095266/1‑53 (MQ=255)

                                                                                           tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGcc                                                                                                                <  2:1095266/53‑1 (MQ=255)

                                                                                           tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAt                                                                                                     >  2:27236/1‑64 (MQ=255)

                                                                                           tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAt                                                                                                     <  1:27236/64‑1 (MQ=255)

                                                                                           tGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAt                          >  2:137140/1‑139 (MQ=255)

                                                                                             gCAAGGCGATATCATTCCATTACGCTAATCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   <  1:591380/112‑1 (MQ=255)

                                                                                             gCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           >  2:1087101/1‑40 (MQ=255)

                                                                                             gCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGcc                                                                                                                           <  1:1087101/40‑1 (MQ=255)

                                                                                             gCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                   >  2:591380/1‑112 (MQ=255)

                                                                                                 ggCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCaa                    <  2:229003/139‑1 (MQ=255)

                                                                                                    gATATCATTCCATTACGCTAAGCTTTTTCGGCCTgg                                                                                                                        >  1:425680/1‑36 (MQ=255)

                                                                                                    gATATCATTCCATTACGCTAAGCTTTTTCGGCCTgg                                                                                                                        <  2:425680/36‑1 (MQ=255)

                                                                                                     atatCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAccac                <  2:384141/139‑3 (MQ=255)

                                                                                                       atCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                            >  1:558341/1‑77 (MQ=255)

                                                                                                       atCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                            <  2:558341/77‑1 (MQ=255)

                                                                                                         cATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGa            <  1:996379/139‑1 (MQ=255)

                                                                                                            tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGc                                                                                  <  2:138587/66‑1 (MQ=255)

                                                                                                            tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGc                                                                                  >  1:138587/1‑66 (MQ=255)

                                                                                                            tCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGcc         >  2:575509/1‑139 (MQ=255)

                                                                                                                ttACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGTCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCt     <  1:1037437/139‑1 (MQ=255)

                                                                                                                ttACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAAAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAATCCCATGAGCCACCt     >  2:1037437/1‑139 (MQ=255)

                                                                                                                  aCGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTgg   <  1:37633/139‑1 (MQ=255)

                                                                                                                   cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGa                                                                                         >  1:250047/1‑52 (MQ=255)

                                                                                                                   cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGa                                                                                         <  2:250047/52‑1 (MQ=255)

                                                                                                                   cGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt  >  1:838235/1‑139 (MQ=255)

TAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT  >  NC_000913/1925976‑1926229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A