Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,798	A→G	intergenic (+152/‑214)	fadL → / → yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,798	0	A	G	100.0%	141.8 / NA	40	intergenic (+152/‑214)	fadL/yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/17); total (23/17)

CAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTT  >  NC_000913/2462681‑2462929

cAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCat                                                                                                                >  1:587196/1‑139 (MQ=255)

  ggTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCatat                                                                                                              >  2:432592/1‑139 (MQ=255)

  ggTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCatat                                                                                                              >  1:721080/1‑139 (MQ=255)

     gACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGATTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAATGGAAAGGCATAtctt                                                                                                           >  2:439445/1‑139 (MQ=255)

     gACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATAtttt                                                                                                           >  1:1015947/1‑139 (MQ=255)

        tttAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAg                                                                                                        >  2:338092/1‑139 (MQ=255)

            aCTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGt                                                                                                    <  2:1034752/139‑1 (MQ=255)

                ccccACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTccg                                                                                                >  1:981921/1‑137 (MQ=255)

                 cccACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTccgc                                                                                               >  1:883207/1‑136 (MQ=255)

                     cTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGt                                                                                           <  2:1050738/139‑1 (MQ=255)

                       tCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTcc                                                                                         >  1:770202/1‑139 (MQ=255)

                             aaaGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCa                                                                                   <  2:770202/139‑1 (MQ=255)

                                    tgtCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTtctc                                                                                    <  1:368767/131‑1 (MQ=255)

                                    tgtCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTtctc                                                                                    >  2:368767/1‑131 (MQ=255)

                                     gtCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGttt                                                                           >  2:617388/1‑139 (MQ=255)

                                        cTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACt                                                                        >  1:680011/1‑139 (MQ=255)

                                        cTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACt                                                                        >  1:22473/1‑139 (MQ=255)

                                        cTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGATTACt                                                                        >  1:878185/1‑139 (MQ=255)

                                           aTTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAag                                                                                >  1:967183/1‑128 (MQ=255)

                                           aTTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAag                                                                                <  2:967183/128‑1 (MQ=255)

                                            ttGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTc                                                                    <  2:1015947/139‑1 (MQ=255)

                                               cTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGc                                                                 <  1:338092/139‑1 (MQ=255)

                                                     tcctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTcc                                                           >  1:11899/1‑139 (MQ=255)

                                                       ctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAg                                                         >  2:377230/1‑139 (MQ=255)

                                                       ctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAg                                                         >  2:195452/1‑139 (MQ=255)

                                                         gAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGa                                                       >  1:987215/1‑139 (MQ=255)

                                                          aGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAt                                                      <  2:414870/139‑1 (MQ=255)

                                                                 cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCTCTCGTCCTTCTCa                                                                                   <  2:745512/103‑1 (MQ=255)

                                                                 cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                               <  1:432592/139‑1 (MQ=255)

                                                                 cAAACAGCCCGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCa                                                                                   >  1:745512/1‑103 (MQ=255)

                                                                   aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                             <  1:377230/139‑1 (MQ=255)

                                                                   aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                             <  2:11899/139‑1 (MQ=255)

                                                                           gTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTa                                    <  2:22473/139‑1 (MQ=255)

                                                                            ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTAt                                   >  1:938184/1‑139 (MQ=255)

                                                                                            tCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                               <  2:177865/127‑1 (MQ=255)

                                                                                            tCAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                               >  1:177865/1‑127 (MQ=255)

                                                                                             cAAATCAATAAGCGGTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTc                  <  2:721080/139‑1 (MQ=255)

                                                                                                           gTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg                             <  2:295896/114‑1 (MQ=255)

                                                                                                           gTATCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTgcg                             >  1:295896/1‑114 (MQ=255)

                                                                                                             aTCCACAGGCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCCGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt  <  2:587196/139‑1 (MQ=255)

CAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTT  >  NC_000913/2462681‑2462929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A