Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,504,006	G→A	intergenic (‑575/‑52)	insG ← / → yjhB	IS4 transposase/putative MFS transporter, membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,504,006	0	G	A	100.0%	136.2 / NA	43	intergenic (‑575/‑52)	insG/yjhB	IS4 transposase/putative MFS transporter, membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/23); total (20/23)

GTATCAGTTTTATCTTTCATAGAGTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGGCTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCATGGCTTGGATA  >  NC_000913/4503870‑4504128

gTATCAGTTTTATCTTTCATAGAGTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACt                                                                                                                          <  2:45154/139‑1 (MQ=255)

   tCAGTTTTATCTTTCATAGAGTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTccc                                                                                                                       >  1:610310/1‑139 (MQ=255)

    cAGTTTTATCTTTCATAGAGTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCt                                                                                                                      <  2:927423/139‑1 (MQ=255)

                 cATAGAGTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTa                                                                                                         >  2:565534/1‑139 (MQ=255)

                     gagTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAggg                                                                                                     <  2:455034/139‑1 (MQ=255)

                     gagTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAggg                                                                                                     <  1:453850/139‑1 (MQ=255)

                          aaaTTTGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAg                                                                                                       <  1:720648/132‑1 (MQ=255)

                          aaaTATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAg                                                                                                       >  2:720648/1‑132 (MQ=255)

                           aaTATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTatat                                                                                               <  2:462736/139‑1 (MQ=255)

                                gTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTcc                                                                                                                        <  1:448878/109‑1 (MQ=255)

                                gTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTcc                                                                                                                        >  2:448878/1‑109 (MQ=255)

                                   aagaagAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATaa                                                                                       <  2:1014238/139‑1 (MQ=255)

                                           aTGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATAtt                                                                                                                >  1:569348/1‑106 (MQ=255)

                                           aTGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATAtt                                                                                                                <  2:569348/106‑1 (MQ=255)

                                                      gATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGc                                                                    <  1:423418/139‑1 (MQ=255)

                                                      gATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTAC‑‑TAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCaa                                                                  <  1:450430/139‑1 (MQ=255)

                                                        ttGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCaa                                                                  <  1:565534/139‑1 (MQ=255)

                                                            aCTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGc                                                              <  2:289399/139‑1 (MQ=255)

                                                             cTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCa                                                             <  1:796177/139‑1 (MQ=255)

                                                                aTTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATgg                                                          >  2:26981/1‑139 (MQ=255)

                                                                                aatcaatAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATc                                          >  1:736228/1‑139 (MQ=255)

                                                                                  tcaatAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGCGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATcca                                        >  2:798264/1‑139 (MQ=255)

                                                                                      tAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAggg                                                                                                     <  1:1086906/74‑1 (MQ=255)

                                                                                      tAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAggg                                                                                                     >  2:1086906/1‑74 (MQ=255)

                                                                                          aCTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACg                                >  1:1058457/1‑139 (MQ=255)

                                                                                           cTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACGGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCcaccac                                    >  1:469372/1‑134 (MQ=255)

                                                                                           cTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACGGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCcaccac                                    <  2:469372/134‑1 (MQ=255)

                                                                                           cTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCa                                                             >  2:184956/1‑109 (MQ=255)

                                                                                           cTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCa                                                             <  1:184956/109‑1 (MQ=255)

                                                                                              gCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGaaa                            >  2:770017/1‑139 (MQ=255)

                                                                                                   tttGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTa                                                                                                         <  1:532774/57‑1 (MQ=255)

                                                                                                   tttGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTa                                                                                                         >  2:532774/1‑57 (MQ=255)

                                                                                                   tttGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGctct                       >  1:638911/1‑139 (MQ=255)

                                                                                                   tttGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGc                          <  1:859820/136‑1 (MQ=255)

                                                                                                   tttGAAGACAATATAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGc                          >  2:859820/1‑136 (MQ=255)

                                                                                                             atatAATTATTTCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCa             <  1:770017/139‑1 (MQ=255)

                                                                                                                        tCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCATGGCt        >  2:470472/1‑133 (MQ=255)

                                                                                                                        tCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCATGGCt        <  1:470472/133‑1 (MQ=255)

                                                                                                                        tCTGGATATTGTTGAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCATGGCTTGGata  >  1:488082/1‑139 (MQ=255)

                                                                                                                                 tgttgAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCa             >  1:26404/1‑119 (MQ=255)

                                                                                                                                 tgttgAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCa             <  2:26404/119‑1 (MQ=255)

                                                                                                                                 tgttgAGACTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCATGGc         >  2:750957/1‑123 (MQ=255)

                                                                                                                                 tgttgAGACTCCCGAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCTTGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCATGGc         <  1:750957/123‑1 (MQ=255)

GTATCAGTTTTATCTTTCATAGAGTGAAATATGTTAAGAAGAAATGGAGGAAAAGATTGTACTGATTAGGTATTGATAACAATCAATAGTACTGGCGTATTTGAAGACAATATAATTATTTCTGGATATTGTTGAGGCTCCCTAATATTTACTTTAAGGGCTATATTAGAATAACACAGGAAACAAATATGGCAACAGCATGGTATAAACAAGTTAATCCACCACAACGGAAAGCTCTTTTTTCCGCATGGCTTGGATA  >  NC_000913/4503870‑4504128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A