Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I1 R1 750 51.0 2595496 93.6% 2429384 106.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 582,152 C→T pseudogene (653/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913582,1520CT100.0% 219.7 / NA 68pseudogene (653/653 nt)ybcYpseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/34);  total (34/34)

GAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGAT  >  NC_000913/582034‑582285
                                                                                                                      |                                                                                                                                     
taaCCTTGTTGATACATCAACTGCACCTGTTATTGAGTGGCCTACGCACCCTGTCAGGGAGTAATCATTGGTATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaa                                                                                                                  <  2:722516/138‑1 (MQ=255)
     gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                             <  2:187569/139‑1 (MQ=255)
      tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                                >  2:1198814/1‑135 (MQ=255)
      tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                                <  1:1198814/135‑1 (MQ=255)
      tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                            >  1:717903/1‑139 (MQ=255)
            tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                      <  1:942437/139‑1 (MQ=255)
                tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                             <  2:1119420/128‑1 (MQ=255)
                tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                             >  1:1119420/1‑128 (MQ=255)
                tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                       <  1:164261/134‑1 (MQ=255)
                tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                       >  2:164261/1‑134 (MQ=255)
                tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                  >  2:100922/1‑139 (MQ=255)
                tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                  >  2:1013304/1‑139 (MQ=255)
                 cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAg                                                                                                                       <  1:397428/117‑1 (MQ=255)
                 cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAg                                                                                                                       >  2:397428/1‑117 (MQ=255)
                 cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAcc                                                                                                 >  2:969662/1‑139 (MQ=255)
                  aaCTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGGCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCt                                                                                                >  2:591557/1‑139 (MQ=255)
                       cACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAc                                                                                           >  2:621548/1‑139 (MQ=255)
                                    gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                      <  2:295173/115‑1 (MQ=255)
                                    gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                      >  1:295173/1‑115 (MQ=255)
                                       gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                       >  2:606729/1‑111 (MQ=255)
                                       gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                       <  1:606729/111‑1 (MQ=255)
                                       gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTCCCTTGAACCTTGGTTTTAACATtc                                                                           >  2:974019/1‑139 (MQ=255)
                                       gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                           >  1:750535/1‑139 (MQ=255)
                                           aCGAACCCTGTCAGGGAGTAATCATTGGGGTTATGGCGCAGCACGTCTTAAGCAAGAACATGGTGCGGTT‑GATGTTATTTTTTTCCTGAAACGAAAAACATTACTAGAGTACCTTGAACCTTGGTTTTAACATTCACGa                                                                       <  2:181430/139‑1 (MQ=255)
                                             gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTCGTTTTAACATTCTCGaaa                                                                     <  2:717903/139‑1 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGa                                                                                                                   <  2:658270/87‑1 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGa                                                                                                                   >  1:658270/1‑87 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                      >  2:748935/1‑100 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                      <  1:748935/100‑1 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               <  1:1013304/139‑1 (MQ=255)
                                                   tGTCAGGGAGTAATCATTGGGATTATGACGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               >  1:648723/1‑139 (MQ=255)
                                                    gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTg                                                              <  1:487884/139‑1 (MQ=255)
                                                     tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                             <  1:621548/139‑1 (MQ=255)
                                                     tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                             <  1:1064213/139‑1 (MQ=255)
                                                           aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                             >  1:601397/1‑133 (MQ=255)
                                                           aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                             <  2:601397/133‑1 (MQ=255)
                                                                     gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCgg                                                                                                                    >  1:681644/1‑68 (MQ=255)
                                                                     gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCgg                                                                                                                    <  2:681644/68‑1 (MQ=255)
                                                                       gATTATGCCGCAGCACGTCTTAAGCAAGAACCTGCTGCGGAT‑GATGTTATTTTTTTCCTGACGCGGAAAACAGTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGcc                                           <  2:980438/139‑1 (MQ=255)
                                                                         ttATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCctt                                         <  2:204220/139‑1 (MQ=255)
                                                                           aTGCCGCAGCACGTCTTAAGCAAGAACGTGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                       >  1:419992/1‑107 (MQ=255)
                                                                           aTGCCGCAGCACGTCTTAAGCAAGAACGTGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                       <  2:419992/107‑1 (MQ=255)
                                                                           aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               >  1:475370/1‑115 (MQ=255)
                                                                           aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               <  2:475370/115‑1 (MQ=255)
                                                                            tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                      >  1:367974/1‑75 (MQ=255)
                                                                            tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                      <  2:367974/75‑1 (MQ=255)
                                                                             gCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCttcttc                                     >  1:576219/1‑139 (MQ=255)
                                                                               cGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCgg                                   <  1:974019/139‑1 (MQ=255)
                                                                               cGCAGCACGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCgg                                   <  2:576219/139‑1 (MQ=255)
                                                                                 cagcaCGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                                 >  1:1015855/1‑139 (MQ=255)
                                                                                      cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               <  1:607349/104‑1 (MQ=255)
                                                                                      cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               >  2:607349/1‑104 (MQ=255)
                                                                                      cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                            >  2:42684/1‑139 (MQ=255)
                                                                                      cGTCTTAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTAT‑TGTGTTAAGCCTTCTTCGGAATCtttt                           >  1:461355/1‑139 (MQ=255)
                                                                                           tAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTgg                                                                                      <  2:955872/76‑1 (MQ=255)
                                                                                           tAAGCAAGAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTgg                                                                                      >  1:955872/1‑76 (MQ=255)
                                                                                                  gAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                                >  1:42363/1‑43 (MQ=37)
                                                                                                  gAACATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                                <  2:42363/43‑1 (MQ=37)
                                                                                                   aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                             <  2:570199/93‑1 (MQ=255)
                                                                                                   aaCATGCTGCGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                             >  1:570199/1‑93 (MQ=255)
                                                                                                       tgctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               <  1:733518/87‑1 (MQ=255)
                                                                                                       tgctgcGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                               >  2:733518/1‑87 (MQ=255)
                                                                                                            cGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAACCATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTc      >  1:1041010/1‑139 (MQ=255)
                                                                                                            cGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                 <  1:483222/80‑1 (MQ=255)
                                                                                                            cGGTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                 >  2:483222/1‑80 (MQ=255)
                                                                                                                ttgATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTAT‑TGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGAt  <  2:461355/137‑1 (MQ=255)
                                                                                                                  gATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGttt                     >  1:1166940/1‑119 (MQ=255)
                                                                                                                  gATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGttt                     <  2:1166940/119‑1 (MQ=255)
                                                                                                                      |                                                                                                                                     
GAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGAT  >  NC_000913/582034‑582285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A