Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
855,796 |
A→G |
intergenic (‑52/‑167) |
ldtB ← / → ybiT |
L,D‑transpeptidase linking Lpp to murein/ABC‑F family putative regulatory ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 855,796 | 0 | A | G | 100.0%
| 92.2
/ NA
| 27 | intergenic (‑52/‑167) | ldtB/ybiT | L,D‑transpeptidase linking Lpp to murein/ABC‑F family putative regulatory ATPase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/14); total (13/14) |
TAAGTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATGCGC > NC_000913/855665‑855931
|
tAAGTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg < 1:1189374/139‑1 (MQ=255)
cGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATtat > 2:19746/1‑139 (MQ=255)
cGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATtat < 1:19746/139‑1 (MQ=255)
cAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTc < 2:843553/139‑1 (MQ=255)
aaaaGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTca > 2:345087/1‑139 (MQ=255)
gCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGt > 1:1126394/1‑139 (MQ=255)
caGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAg < 2:1126394/139‑1 (MQ=255)
tCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCt < 1:854539/139‑1 (MQ=255)
cATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCtt < 2:358515/139‑1 (MQ=255)
aTGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTa > 1:685105/1‑139 (MQ=255)
aaGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg < 1:1069131/56‑1 (MQ=255)
aaGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg > 2:1069131/1‑56 (MQ=255)
cGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAgcgc < 2:1282109/56‑1 (MQ=255)
cGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAgcgc > 1:1282109/1‑56 (MQ=255)
tgCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAgcgc < 2:602768/46‑1 (MQ=255)
tgCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAgcgc > 1:602768/1‑46 (MQ=255)
ggCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCtt < 1:380499/109‑1 (MQ=255)
ggCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCtt > 2:380499/1‑109 (MQ=255)
gCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGAtc < 1:265934/134‑1 (MQ=255)
gCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGAtc > 2:265934/1‑134 (MQ=255)
gagaATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATg > 2:295578/1‑139 (MQ=255)
tAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATg > 2:1060002/1‑139 (MQ=255)
nagNNNNGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcg < 2:452664/138‑1 (MQ=255)
gagTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcg < 1:345087/139‑1 (MQ=255)
gagTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcg < 1:194832/139‑1 (MQ=255)
agTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATGAGATGTGTACGAAATGACATTATTTGCCTTTGGCTTGAGTGTAGACGTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcgc > 2:754272/1‑139 (MQ=255)
agTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAg < 1:140014/48‑1 (MQ=255)
agTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAg > 2:140014/1‑48 (MQ=255)
|
TAAGTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATGCGC > NC_000913/855665‑855931
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
