Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,149,712 |
G→A |
*318* (TAG→TAA) |
fabH → |
3‑oxoacyl‑[acyl‑carrier‑protein] synthase III |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,149,712 | 0 | G | A | 100.0%
| 93.0
/ NA
| 30 | *318* (TAG→TAA) | fabH | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase III |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/18); total (12/18) |
TAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCT > NC_000913/1149577‑1149846
|
tAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCCTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGAt < 2:1169729/139‑1 (MQ=255)
aaTACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa < 1:502250/139‑1 (MQ=255)
ctctGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGAt < 1:996083/139‑1 (MQ=255)
gCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTaaa > 2:81499/1‑139 (MQ=255)
tGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACg < 1:1199319/139‑1 (MQ=255)
cgcgcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCa > 1:1174097/1‑139 (MQ=255)
gcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGAt > 1:1131727/1‑109 (MQ=255)
gcTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGAt < 2:1131727/109‑1 (MQ=255)
tGTACTCGACGGGCGCATTAGGCCGGGGCAGTTGGTTCTGCTTTAAGCCTGTGGCGGTGAATTCACCTGGGGGTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATTACGCAATTTGCATTTGTGTTCCCTGGa < 1:635625/139‑1 (MQ=255)
tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGAt < 1:281239/95‑1 (MQ=255)
tACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGAt > 2:281239/1‑95 (MQ=255)
aCGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAg < 2:516203/139‑1 (MQ=255)
cGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTcc > 2:755779/1‑125 (MQ=255)
cGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTcc < 1:755779/125‑1 (MQ=255)
ggCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCa < 2:399901/139‑1 (MQ=255)
gcgcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAAt > 2:1023279/1‑107 (MQ=255)
gcgcATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAAt < 1:1023279/107‑1 (MQ=255)
ttGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATggcggc < 1:81499/139‑1 (MQ=255)
cTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa < 2:44940/50‑1 (MQ=255)
cTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATa > 1:44940/1‑50 (MQ=255)
tGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATaa > 1:831013/1‑42 (MQ=255)
tGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAAGATaa < 2:831013/42‑1 (MQ=255)
ccacgcTGGTTGGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTGGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGa > 2:749867/4‑139 (MQ=255)
ccGCGCTGGTTCTTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGt < 2:950651/132‑1 (MQ=255)
ccGCGCTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGt > 1:950651/1‑132 (MQ=255)
gcgcTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCa < 1:666209/48‑1 (MQ=255)
gcgcTGGTTCGTTTCTAAGATAAGGATTAAAACATGACGCAATTTGCa > 2:666209/1‑48 (MQ=255)
ttCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTcc > 2:372265/1‑46 (MQ=255)
ttCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTcc < 1:372265/46‑1 (MQ=255)
tCTAAGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCt < 2:910056/139‑1 (MQ=255)
|
TAATACCTCTGCGGCCTCTGTCCCGTGCGCGCTGGATGAAGCTGTACGCGACGGGCGCATTAAGCCGGGGCAGTTGGTTCTGCTTGAAGCCTTTGGCGGTGGATTCACCTGGGGCTCCGCGCTGGTTCGTTTCTAGGATAAGGATTAAAACATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCT > NC_000913/1149577‑1149846
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
