Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,153,047	A→G	D370G (GAT→GGT)	fabF →	3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,153,047	0	A	G	100.0%	115.5 / NA	33	D370G (GAT→GGT)	fabF	3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/16); total (17/16)

TGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAA  >  NC_000913/1152925‑1153171

tgtgtTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGt                                                                                                              >  1:801881/1‑139 (MQ=255)

                       cTATGACCGGTCGCCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCATTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCa                                                                                       >  1:572054/1‑139 (MQ=255)

                         aTGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAAACTATCTACTCCATCCTGGCGCTGCGCGATCAGGGTGTTCCGACAACCATCAAACTGGGTAAAACGGACGAAGGGTGCGATCTGGATTTCGGACGGCACg                                                                                     >  1:244572/1‑139 (MQ=255)

                                gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCCTCAACCTGGGTAACCCGGATGAATGTTGCGa                                                                                                         >  2:512381/1‑112 (MQ=255)

                                gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGa                                                                                                         <  1:512381/112‑1 (MQ=255)

                                gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                              >  2:659167/1‑139 (MQ=255)

                                 tCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGt                                                                             <  2:801881/139‑1 (MQ=255)

                                         tAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAg                                                                     <  2:158708/139‑1 (MQ=255)

                                           ggTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCg                                                                   <  2:572054/139‑1 (MQ=255)

                                             tGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                 <  2:607244/139‑1 (MQ=255)

                                             tGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                 <  2:252548/139‑1 (MQ=255)

                                               cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                               <  2:244572/139‑1 (MQ=255)

                                                         aGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                     <  2:213701/139‑1 (MQ=255)

                                                                tctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTa                                              >  1:53385/1‑139 (MQ=255)

                                                                   atctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACt                                           >  1:597962/1‑139 (MQ=255)

                                                                     ctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTcc                                         >  1:276915/1‑139 (MQ=255)

                                                                        cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatggaat                                                          <  1:707224/119‑1 (MQ=255)

                                                                        cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatggaat                                                          >  2:707224/1‑119 (MQ=255)

                                                                        cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                     <  1:135868/124‑1 (MQ=255)

                                                                        cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                     >  2:135868/1‑124 (MQ=255)

                                                                             tCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggctt                                 >  1:176798/1‑139 (MQ=255)

                                                                                    gcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGc                          <  2:716488/139‑1 (MQ=255)

                                                                                        cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                      >  2:506765/1‑139 (MQ=255)

                                                                                        cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                      >  1:333405/1‑139 (MQ=255)

                                                                                         gcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTaa                     <  1:659167/139‑1 (MQ=255)

                                                                                             tCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                 >  1:633292/1‑91 (MQ=255)

                                                                                             tCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                 <  2:633292/91‑1 (MQ=255)

                                                                                                  cTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                     <  1:679919/98‑1 (MQ=255)

                                                                                                  cTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                     >  2:679919/1‑98 (MQ=255)

                                                                                                     ttCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGAt         >  1:278617/1‑139 (MQ=255)

                                                                                                      tCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatggaa                                                           >  2:719806/1‑88 (MQ=255)

                                                                                                      tCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatggaa                                                           <  1:719806/88‑1 (MQ=255)

                                                                                                            aaCCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaa  <  1:506765/139‑1 (MQ=255)

TGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAA  >  NC_000913/1152925‑1153171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A