Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I1 R1 759 71.0 3673240 91.5% 3361014 104.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,153,047 A→G D370G (GAT→GGT)  fabF → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,153,0470AG100.0% 226.5 / NA 64D370G (GAT→GGT) fabF3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/30);  total (34/30)

AGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAG  >  NC_000913/1152913‑1153174
                                                                                                                                       |                                                                                                                               
aGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAAc                                                                                                                             >  1:799244/1‑139 (MQ=255)
 gCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAAcc                                                                                                                            <  2:799244/139‑1 (MQ=255)
  cTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAAacc                                                                                                                           >  1:1464138/1‑139 (MQ=255)
      aaGCCGTGTGTTGGTAAGCTCCACGAAATCTATGTCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGAt                                                                                                                       <  2:1116782/139‑1 (MQ=255)
            tgtgtTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGt                                                                                                                 >  2:1276502/1‑139 (MQ=255)
                 tGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGa                                                                                                            <  2:1559653/139‑1 (MQ=255)
                   gTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATc                                                                                                          >  1:434966/1‑139 (MQ=255)
                        cTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACCACATCTTGG‑GCTGGCGCCTACAGGCTGTTTGGCCAACCATCAACCTTGGTAACCCGGATGAAGGTTCGGATTTGGAt                                                                                                     >  1:301274/1‑139 (MQ=255)
                           cACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTc                                                                                                  >  1:1553212/1‑139 (MQ=255)
                                       gACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGaa                                                                                      >  2:1815537/1‑139 (MQ=255)
                                        aCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                     <  2:967203/139‑1 (MQ=255)
                                        aCCGGTCAACTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                     <  2:301274/139‑1 (MQ=255)
                                          cGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCg                                                                                                             <  2:609355/113‑1 (MQ=255)
                                          cGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCg                                                                                                             >  1:609355/1‑113 (MQ=255)
                                            gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGATGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                                 >  2:1106529/1‑139 (MQ=255)
                                            gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGtt                                                                                                                >  2:344207/1‑108 (MQ=255)
                                            gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGtt                                                                                                                <  1:344207/108‑1 (MQ=255)
                                            gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                                 <  2:434966/139‑1 (MQ=255)
                                            gTCACCTGTTAGGTGCGGCGGGGGCAGTAAAATTTTCCTACCCCATCCTGGGCCT‑CCCCGACCAGGCTTTTCCCCCAACCATCACCCTGGGTAACCCGGAGGAAGGTTGCGATCGGGATTTGTTCCCCCACGAAggggt                                                                                 >  1:594655/1‑135 (MQ=255)
                                             tCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGt                                                                                <  1:1106529/139‑1 (MQ=255)
                                             tCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGt                                                                                <  1:1815537/139‑1 (MQ=255)
                                                           cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAgg                                                                                                                  <  2:307922/91‑1 (MQ=255)
                                                           cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAgg                                                                                                                  >  1:307922/1‑91 (MQ=255)
                                                                     aGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                        <  2:1801146/139‑1 (MQ=255)
                                                                                    cTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTa                                                 <  2:1235665/131‑1 (MQ=255)
                                                                                    cTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTa                                                 >  1:1235665/1‑131 (MQ=255)
                                                                                    cTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttc                                         >  2:1024030/1‑139 (MQ=255)
                                                                                    cTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttc                                         >  2:1696486/1‑139 (MQ=255)
                                                                                     tCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGAt                                                                                                                       >  1:870022/1‑60 (MQ=255)
                                                                                     tCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGAt                                                                                                                       <  2:870022/60‑1 (MQ=255)
                                                                                     tCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcg                                        >  1:647183/1‑139 (MQ=255)
                                                                                       cATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggc                                      <  1:1024030/139‑1 (MQ=255)
                                                                                         tCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTa                                                                                               >  1:1361806/1‑80 (MQ=255)
                                                                                         tCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTa                                                                                               <  2:1361806/80‑1 (MQ=255)
                                                                                          ccTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGAtt                                                                                                    >  1:1604580/1‑74 (MQ=255)
                                                                                          ccTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGAtt                                                                                                    <  2:1604580/74‑1 (MQ=255)
                                                                                          ccTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggcttc                                   >  2:979983/1‑139 (MQ=255)
                                                                                          ccTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggcttc                                   >  2:1489296/1‑139 (MQ=255)
                                                                                            tGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggcttcgg                                 <  2:647183/139‑1 (MQ=255)
                                                                                             ggCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGt                                <  1:979983/139‑1 (MQ=255)
                                                                                              gcgcT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTg                               <  1:1696486/139‑1 (MQ=255)
                                                                                              gcgcT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTGCGGCTTCGGTg                               >  2:1186299/1‑139 (MQ=255)
                                                                                               cgcT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGAt                                                                                                                       >  1:1336734/1‑50 (MQ=255)
                                                                                               cgcT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGAt                                                                                                                       <  2:1336734/50‑1 (MQ=255)
                                                                                                    gcgcGATCAGACTGTTCCGCCAACCATCAACCTGGGTAACCCGGAt                                                                                                                       >  2:1483933/1‑46 (MQ=255)
                                                                                                    gcgcGATCAGACTGTTCCGCCAACCATCAACCTGGGTAACCCGGAt                                                                                                                       <  1:1483933/46‑1 (MQ=255)
                                                                                                     cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAg                                                                        <  1:1122405/92‑1 (MQ=255)
                                                                                                     cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAg                                                                        >  2:1122405/1‑92 (MQ=255)
                                                                                                     cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                         >  1:1595950/1‑139 (MQ=255)
                                                                                                     cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                         >  1:934508/1‑139 (MQ=255)
                                                                                                     cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                         >  2:689464/1‑139 (MQ=255)
                                                                                                             ggCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCt                 >  2:1799761/1‑139 (MQ=255)
                                                                                                                    ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatggaa                                                              >  1:791650/1‑87 (MQ=255)
                                                                                                                    ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatggaa                                                              <  2:791650/87‑1 (MQ=255)
                                                                                                                    ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGc                             <  1:1800467/120‑1 (MQ=255)
                                                                                                                    ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGc                             >  2:1800467/1‑120 (MQ=255)
                                                                                                                    ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCt          <  2:594655/139‑1 (MQ=255)
                                                                                                                      gCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                         >  1:1702642/1‑122 (MQ=255)
                                                                                                                      gCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                         <  2:1702642/122‑1 (MQ=255)
                                                                                                                         aaCCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaa     <  1:1276502/139‑1 (MQ=255)
                                                                                                                         aaCCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaa     <  2:1464138/139‑1 (MQ=255)
                                                                                                                            cATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                        <  2:1334962/85‑1 (MQ=255)
                                                                                                                            cATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                        >  1:1334962/1‑85 (MQ=255)
                                                                                                                            cATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAg  >  2:171967/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                               
AGCTGCAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCT‑GCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAG  >  NC_000913/1152913‑1153174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A