Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 765 46.1 2269152 93.8% 2128464 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,153,047 A→G D370G (GAT→GGT)  fabF → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,153,0470AG100.0% 204.4 / NA 57D370G (GAT→GGT) fabF3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)

CAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAA  >  NC_000913/1152918‑1153171
                                                                                                                                 |                                                                                                                            
cAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGa                                                                                                                     >  2:279665/1‑139 (MQ=255)
  aGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATg                                                                                                                   >  1:329484/1‑139 (MQ=255)
          gtTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGc                                                                                                           <  1:279665/139‑1 (MQ=255)
               tAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                      >  1:536429/1‑139 (MQ=255)
                     ccACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGAttt                                                                                                >  2:418004/1‑139 (MQ=255)
                         gAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTa                                                                                            <  1:352077/139‑1 (MQ=255)
                                   aCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  >  2:735562/1‑139 (MQ=255)
                                   aCCGGTCACCTCTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  <  1:735562/139‑1 (MQ=255)
                                       gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCg                                                                                                                       <  1:981644/98‑1 (MQ=255)
                                       gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                      >  1:138335/1‑115 (MQ=255)
                                       gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                      <  2:138335/115‑1 (MQ=255)
                                       gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                              >  1:426480/1‑139 (MQ=255)
                                       gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAAACATCAACCTGGGTAACCCg                                                                                                                       >  2:981644/1‑98 (MQ=255)
                                         cACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGtt                                                                                                             >  1:329299/1‑106 (MQ=255)
                                         cACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGtt                                                                                                             <  2:329299/106‑1 (MQ=255)
                                              gTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  <  2:827054/128‑1 (MQ=255)
                                              gTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  >  1:827054/1‑128 (MQ=255)
                                                tAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAg                                                                     <  2:446746/139‑1 (MQ=255)
                                                    tGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                 <  1:418004/139‑1 (MQ=255)
                                                    tGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                 <  1:979820/139‑1 (MQ=255)
                                                      cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTAc                                                                                           <  1:503417/111‑1 (MQ=255)
                                                      cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTAc                                                                                           >  2:503417/1‑111 (MQ=255)
                                                         cggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  <  2:833559/117‑1 (MQ=255)
                                                         cggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  <  2:25708/117‑1 (MQ=255)
                                                         cggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  >  1:25708/1‑117 (MQ=255)
                                                         cggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  >  1:833559/1‑117 (MQ=255)
                                                         cggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatgga                                                            >  2:928787/1‑139 (MQ=255)
                                                              gCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATaca                                                       <  1:928787/139‑1 (MQ=255)
                                                                aGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                     <  2:536429/139‑1 (MQ=255)
                                                                       tctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAAcc                                                                                                                         >  1:17919/1‑64 (MQ=255)
                                                                       tctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAAcc                                                                                                                         <  2:17919/64‑1 (MQ=255)
                                                                        ctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTaa                                             <  2:144746/139‑1 (MQ=255)
                                                                          atctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACt                                           >  2:21378/1‑139 (MQ=255)
                                                                            ctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGt                                                                                             >  2:1104807/1‑87 (MQ=255)
                                                                            ctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGt                                                                                             <  1:1104807/87‑1 (MQ=255)
                                                                               cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGAt                                                                                                                    <  1:1094191/61‑1 (MQ=255)
                                                                               cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGAt                                                                                                                    >  2:1094191/1‑61 (MQ=255)
                                                                               cTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttc                                      >  2:764808/1‑139 (MQ=255)
                                                                                  cATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                     >  2:732348/1‑121 (MQ=255)
                                                                                  cATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                     <  1:732348/121‑1 (MQ=255)
                                                                                       tGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggcttcgg                              >  2:412260/1‑139 (MQ=255)
                                                                                           gcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGc                          <  2:329484/139‑1 (MQ=255)
                                                                                              gcgcGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCct                                        <  2:552570/122‑1 (MQ=255)
                                                                                              gcgcGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCct                                        >  1:552570/1‑122 (MQ=255)
                                                                                               cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                      >  1:1121031/1‑59 (MQ=255)
                                                                                               cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                      <  2:1121031/59‑1 (MQ=255)
                                                                                               cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                              <  2:363719/83‑1 (MQ=255)
                                                                                               cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                              >  1:363719/1‑83 (MQ=255)
                                                                                               cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                      >  1:704623/1‑139 (MQ=255)
                                                                                                  gATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATg                   <  2:704623/139‑1 (MQ=255)
                                                                                                              ccGCCAACCATCAACCTGGGTAACGCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGa          >  2:767133/1‑136 (MQ=255)
                                                                                                              ccGCCAACCATCAACCTGGGTAACCCCGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATCGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGa          <  1:767133/136‑1 (MQ=255)
                                                                                                                   aaCCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaa  <  2:426480/139‑1 (MQ=255)
                                                                                                                      cATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggc                                   <  2:408811/103‑1 (MQ=255)
                                                                                                                      cATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggc                                   >  1:408811/1‑103 (MQ=255)
                                                                                                                      cATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATg                   <  1:576110/119‑1 (MQ=255)
                                                                                                                      cATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATg                   >  2:576110/1‑119 (MQ=255)
                                                                                                                                 |                                                                                                                            
CAAGCCGTGTGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAA  >  NC_000913/1152918‑1153171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A